Transcript Cherry leaf roll disease - Washington State University

Cherry leaf roll disease
Sample Collection & Analysis
National Clean Plant Network – Fruit Trees
Types of samples to collect
Budsticks
• Collect from the end of January
until bud break
• About 8” at the base of the
1-year-old wood
• One stick from each scaffold or
symptomatic limb
National Clean Plant Network- Fruit Trees
Types of samples to collect
Flower & leaf buds
• Bud swell to flower bud popcorn
stage
• Six buds in total
• Collect from all major limbs and
branches
National Clean Plant Network- Fruit Trees
Types of samples to collect
Leaves
• Collect 2 weeks after full bloom
until the end of June
• Collect the first leaves that
emerge in spring (oldest)
• Collect six leaves in total from
each tree
• Collect from all major scaffold
limbs including any symptomatic
limbs
National Clean Plant Network- Fruit Trees
Collect samples from all major limbs
• Virus distribution is erratic,
particularly with recent
infections
• Insure that part of each
sample is collected from
symptomatic shoots or from
each major limb
National Clean Plant Network- Fruit Trees
Ship samples in ziplock bags
• Seal samples in ziplock bags
(don’t add extra moisture)
• Label bag (tree, row, grower,
GPS)
• Include contact information
• Keep out of direct sun
• Keep cool (40F), but don’t freeze
• Overnight delivery to
WSU ELISA Lab
(early to mid-week)
National Clean Plant Network- Fruit Trees
Shipping Information
Ship overnight delivery via courier to:
ELISA Laboratory
Washington State
University-IAREC
24106 N. Bunn Rd.
Prosser, WA 99350
Switchboard: 509-786-9382
Facsimile: 509-786-9370
Contact:
Tina Vasile
ELISA Lab Manager
509-786-9382
[email protected]
Ken Eastwell
Project Manager
509-786-9385
[email protected]
National Clean Plant Network- Fruit Trees