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SCHOTT
SCHOTT NEXTERION MTP-96
Maximum & Minimum filling volumes per well
SCHOTT TGS
Experimental Details
 Substrate tested:
- MTP 2-2259 without patterning 18.01.11
 Buffer Solutions tested:
- PBS (1x) with 0.08 mg/ml Dye 5
- PBST 0.1% Tween with 0.08 mg/ml Dye 5
Corinna Schwung, 20.07.2015
© SCHOTT Technical Glass Solutions GmbH
PRODUCT GROUP NEXTERION
SCHOTT TGS
PRODUCT GROUP NEXTERION
Filling Scheme
1
2
3
4
5
6
7
8
9
10
11
12
A
130
140
150
160
170
180
190
200
210
220
230
240
B
130
140
150
160
170
180
190
200
210
220
230
240
C
10
20
30
40
50
60
70
80
90
100
110
120
D
10
20
30
40
50
60
70
80
90
100
110
120
E
10
20
30
40
50
60
70
80
90
100
110
120
F
10
20
30
40
50
60
70
80
90
100
110
120
G
130
140
150
160
170
180
190
200
210
220
230
240
H
130
140
150
160
170
180
190
200
210
220
230
240
PBST
Volume in µl
PBS
Corinna Schwung, 20.07.2015
© SCHOTT Technical Glass Solutions GmbH
These wells were filled up to
maximum liquid level.
SCHOTT TGS
PRODUCT GROUP NEXTERION
Results from adding 10 – 120 µl per well
1
2
3
4
A
5
6
7
8
9
10
11 12
- Solution pipetted into upper left
corner
PBST
B
- MTP was gently shaken
C
Minimum Volumes
D
- PBST: 40 µl
- PBS: 50 µl
E
F
G
PBS
H
10 µl
Corinna Schwung, 20.07.2015
© SCHOTT Technical Glass Solutions GmbH
120 µl
SCHOTT TGS
PRODUCT GROUP NEXTERION
Results from adding 10 – 240 µl per well
1
2
3
4
5
6
7
130 µl
A
8
9
10
11 12
240 µl
PBST
B
C
PBST
D
10 µl
120 µl
E
PBS
F
G
PBS
H
130 µl
Corinna Schwung, 20.07.2015
© SCHOTT Technical Glass Solutions GmbH
240 µl
SCHOTT TGS
PRODUCT GROUP NEXTERION
Results from adding 10 – 240 µl per well
- Safe filling volume is about 160 µl for PBS, 130 µl for PBST
PBST
PBS
PBS
PBST
Corinna Schwung, 20.07.2015
© SCHOTT Technical Glass Solutions GmbH
SCHOTT TGS
PRODUCT GROUP NEXTERION
Results from adding 10 – 240 µl per well
PBS
PBST
Corinna Schwung, 20.07.2015
© SCHOTT Technical Glass Solutions GmbH
- The maximum filling level was
not reached with these volumes
SCHOTT TGS
PRODUCT GROUP NEXTERION
Results from adding 320 – 400 µl per well
PBS
Maximum Volume per
well
PBST
- PBS: 410 µl
- PBST: 340 µl
Corinna Schwung, 20.07.2015
© SCHOTT Technical Glass Solutions GmbH
SCHOTT TGS
PRODUCT GROUP NEXTERION
Conclusions
 The minimum volume that will cover the well bottom is:
 40 microlitres (with detergent)
 50 microlitres (without detergent)
 The maximum recommended „safe“ working volume is:
 130 microlitres (with detergent)
 160 microlitres (without detergent)
Corinna Schwung, 20.07.2015
© SCHOTT Technical Glass Solutions GmbH
SCHOTT
SCHOTT NEXTERION MTP-96
Maximum & Minimum filling volumes per well