Transcript SNC1D1 – ECOSYSTEMS UNIT

PARTS OF THE COMPOUND LIGHT MICROSCOPE
revolving nosepiece
arm
diaphragm
ocular lens / eyepiece
condenser
objective lenses
stage clips
body tube
base
light source / illuminator
coarse adjustment knob
stage
fine adjustment knob
PARTS OF THE COMPOUND LIGHT MICROSCOPE
ocular lens/eyepiece
body tube
revolving/rotating nosepiece
objective lenses
arm
stage clips
stage
condenser
diaphragm
coarse adjustment knob
fine adjustment knob
light source/
illuminator
base
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Ocular Lens / Eyepiece
magnify the image of
• Contains a lens to _______
the specimen.
10 X.
• The usual magnification is ___
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Ocular Lens / Eyepiece
magnify the image of
• Contains a lens to _______
the specimen.
10 X.
• The usual magnification is ___
• Some microscopes have two
___ ocular lenses.
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Body Tube
connects the eyepiece to the objective
• It ________
lenses.
• It ensures the correct alignment
________ of the
microscope components to correctly
direct the light from the specimen into
_____
the viewer’s eye.
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Arm
connects the body tube to the base.
• It ________
• One hand
____ should be around the arm when
_______
carrying the microscope (the other should
be under the base
____).
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Arm
connects the body tube to the base.
• It ________
• One hand
____ should be around the arm when
_______
carrying the microscope (the other should
be under the base
____).
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Base
• It ________
supports the weight of the microscope.
light source
• It contains the electronics
_________ and __________.
• One hand should be under
_____ the base
while _______
carrying the microscope (the other
hand should be holding the arm).
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Base
• It ________
supports the weight of the microscope.
light source
• It contains the electronics
_________ and __________.
• One hand should be under
_____ the base
while _______
carrying the microscope (the other
hand should be holding the arm).
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Light Source / Illuminator
• It sends light upwards
_______ through the
condenser lens and through the ____
hole in
_____________
the stage onto the specimen
________ on the slide.
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Light Source / Illuminator
mirror
• It sends light upwards
_______ through the
•
condenser lens and through the ____
hole in
_____________
the stage onto the specimen
________ on the slide.
Older microscopes used to use mirrors
______ to
______
reflect the ambient light upwards.
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Revolving/Rotating Nose Piece
lenses are attached to it.
• Theobjective
_____________
• Rotating
_______ the nose piece allows you to
switch
_____ between the different lenses.
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Objective Lenses
4X
10 X
40 X
• These lenses further magnify
_______ the image
of the specimen.
4 X , 10
X
• The magnifications are usually ___
____
40 X .
and ____
3 lenses
• There are usually __
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Objective Lenses
• These lenses further magnify
_______ the image
of the specimen.
4 X , 10
X
• The magnifications are usually ___
____
40 X .
and ____
3 lenses but some have __
• There are usually __
4 lenses.
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Objective Lenses
• As the power increases, the magnification
becomes larger
_____ , but the field of view
smaller
(visible area) becomes _______.
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Coarse Adjustment Knob
first knob you should use, and always
• The ____
high power.
under low
___ power. Never use it in ____
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Fine Adjustment Knob
first knob you should use, and always
• The ____
•
high power.
under low
___ power. Never use it in ____
The second
______ knob you should use under
higher power for _____
______
exact focusing.
stage up and down
• Both knobs move the _____
to help put the specimen in focus
_____.
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Fine Adjustment Knob
• Some microscopes have the two knobs
on top of the other
located one
___________________.
• The smaller one on the bottom is always
fine adjustment knob.
the ____
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Stage
slide
• The stage is where you place the ____
•
which contains the specimen
________.
hole that allows ____
It contains a ____
light to pass
through the stage and onto the specimen.
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Stage Clips
slide
• The stage is where you place the ____
•
which contains the specimen
________.
hole that allows ____
It contains a ____
light to pass
through the stage and onto the specimen.
secure the slide on the
• The stage clips ______
stage.
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Condenser Lens
• The lens under the stage that
focuses light from the illuminator
___________
hole in the stage.
through to the ____
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Diaphragm
• The lens under the stage that
focuses light from the illuminator
___________
hole in the stage.
through to the ____
• It contains a dial that rotates to adjust
_____ the
amount of light that reaches the specimen.
_____________
Making a Wet-Mount Slide
1. Place 2-3 drops of
liquid in the center of
the slide
2. Add specimen to the
liquid
3. Add cover slip at a
45 degree angle
Created by Anh-Thi Tang – Tangstar Science
Copyright © April 2013 Anh-Thi Tang (a.k.a. Tangstar Science)
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