Transcript Rapid detection of sperm : comparison of two methods

Rapid detection of sperm
: comparison of two methods
Miss
Wimolmas Phorassamee 51312344
Introduction
comparison of two methods
• Acid phosphatase test
• Zinc test
Component of sperm
Acid
Spermatozoa
Enzyme (Protease, phosphatase)
Semen
Protein
Seminal plasma
Enzyme
Method
174 cervicovaginal samples from consenting women
Cytology
Acid phosphatase test
Zinc test
Statistical
Result
Result (con.)
Result (con.)
 Acid phosphatase
- sensitivity
- specificity
- PPV
- NPV
95%
96%
93%
98%
Result (con.)
 Zinc test
- sensitivity
- specificity
- PPV
- NPV
81%
54%
49%
84%
Discussion & Conclusion
• the acid phosphatase test has good sensitivity and
specificity, and above all that it has a negative predictive
value of 98%
• It is thus valuable as a screening test
• the longest timespan after which detection was possible
was 106 h between intercourse and sampling
Discussion & Conclusion