Transcript Influence of trace elements in nitrification

Influence of trace
elements in nitrification
09/07/2009
Khelifi Faiza
1
•
•
•
•
•
Introduction
Nitrification: What is it and why is it important?
Problems met by OASEN Company
Batch experiment
Conclusions
09/07/2009
Khelifi Faiza
2
Different ways to remove ammonium:
• Break point chlorination (not used in the
Netherlands)
• Adsorption of ammonium on zeolites, ions
exchange
• Stripping
• Biological conversion: Nitrification
09/07/2009
Khelifi Faiza
3
Nitrification
• Biological oxydation of ammonium into nitrite
and then nitrate.
• NH3 + 1,5 O2 + nitrosomonas  NO2- +H2O + H+
NO2- + 0,5 O2 + nitrospira  NO3-
09/07/2009
Khelifi Faiza
4
Why is it important?
• pH > 8 => NH3 formation
• Nitrite formation
 Poisonous for fish
 Blue Baby syndrom
• NH4: Substrate for many
bacteria
09/07/2009
Khelifi Faiza
5
Ammonium in filter effluent [ppm NH
4
]
Ammonium in filter effluent
09/07/2009
6
5
4
3
2
1
0
Oct, 1997
Oct, 1998
Khelifi Faiza
Oct, 1999
Oct, 2000
6
Batch experiment
*Aeration
*T=12°C
*Shaking
09/07/2009
Khelifi Faiza
7
The results expected:
• NH4+ decrease more quickly in bottles with
trace elements.
• NO3- and NO2- increase more in bottles
with trace element than in bottles without
trace elements.
09/07/2009
Khelifi Faiza
8
NO3- and NO2Bach experiment: (N-NO3 + N-NO2)in in 2nd
filtrate Lekkerkerk
4
12
3,5
3
without trace
elements
with trace
elements
2,5
2
1,5
1
0,5
(N-NO3 + N-NO2)
(mg)
(N-NO3+N-NO2) (mg)
Bach experiment:
(N-NO3 + N-NO2) in Lekkerkerk drinking water
10
without trace
elements
with trace
elements
8
6
4
2
0
0
0
5
10
15
20
0
25
5
15
20
25
time (days)
time (days)
(N-NO3 + N-NO2) in 2nd filtrate of ZS
Ridderkerk
Bach experiment: (N-NO3 + N-NO2) in first
filtrate ZS Kamerik
8
3,5
3
without trace
elements
with trace
elements
2,5
2
1,5
1
0,5
(N-NO3 + N-NO2)
(mg)
4
(N-NO3 + N-NO2)
(mg)
10
7
6
without trace
elements
with trace
elements
5
4
3
2
1
0
0
0
5
10
15
20
0
25
10
15
20
25
time (days)
time (days)
09/07/2009
5
Khelifi Faiza
9
Total nitrogen
total nitrogen in Lekkerkerk 2nd filtrate
16
14
12
10
8
6
4
2
0
without trace
elements
with trace elements
total nitrogen
(mg)
total nitrogen
(mg)
total nitrogen in Lekkerkerk drinking water
16
14
12
10
8
6
4
2
0
without trace
elements
with trace elements
0
0
5
10
15
20
5
without trace
elements
with trace elements
15
20
total nitrogen
(mg)
total nitrogen
(mg)
16
14
12
10
8
6
4
2
0
10
25
16
14
12
10
8
6
4
2
0
without trace
elements
with trace elements
0
25
5
10
15
20
25
time (days)
time (days)
09/07/2009
20
total nitrogen in 2nd filtrate Ridderkerk
total nitrogen in first filtrate ZS Kamerik
5
15
time (days)
time (days)
0
10
25
Khelifi Faiza
10
Second experiment
*T =20°C
*Shaking
*Single
limitation
09/07/2009
Khelifi Faiza
11
Results
Batch experiment:
NH4-N in Lekkerkerk 2nd filtrate
without Co
20
15
10
5
0
0
2
4
6
8
with all trace
elements
without trace
elements
(NH4-N) (mg)
(NO3 + NO2)-N
(mg)
Batch experiment:
(NO3 + NO2)-N in Lekkerkerk 2nd filtrate
12
10
8
6
4
2
0
with all trace
elements
without trace
elements
0
time (days)
09/07/2009
without Co
2
4
6
8
time (days)
Khelifi Faiza
12
Total nitrogen
without Co
Total nitrogen (mg)
Batch experiment: Total nitrogen in Lekkerker 2nd filtrate
without P
20
18
16
14
12
10
8
6
4
2
0
without Mo
without Cu
h
without Zn
0
2
4
6
time (days)
09/07/2009
Khelifi Faiza
8
with all trace
elements
without trace
elements
13
Conclusion
09/07/2009
Khelifi Faiza
14
Thank you for your attention,
Questions ?
09/07/2009
Khelifi Faiza
15