Transcript Marker Aided Selection for cooking quality and drought

Marker Aided Selection for
cooking quality and drought
tolerance in Cambodia
By
Heang Dany and Then Rathmuny
Cambodian Agricultural Research and Development Institute
(CARDI)
Introduction
• Quality always requite by human being
• Drought still a major problem in rainfed
lowland rice
Materials and Methods
• Plant materials:
Phka Rum Duol
x
CAR3
F1 x CAR3
BC1F1 x CAR3

BC1F2
BC2F1 x CAR3
BC3F1

BC3F2
QTL location on the map
AC and GC
PSS-II-2
GT
Lanceras et al., 2000. DNA Research 7:93-101
QTL location on the map
GT
QTL location on the map
• Chromosome 8
RG1
BADH Aroma
RG28
Marker used
Trait
Marker
Chromosome
Amylose content
RM597
6
And Gel consistency
RM589
6
RM190
RM587
Waxy
6
6
6
RM204
RM225
BADH
6
6
8
Aroma
Marker used
Gelatinization Temperature RM111
RM50
RM121
PSS-II-1
PSS-II-2
RM136
GT11
Gelatinization Temperature RM229
RM457
RM21
RM473E
RM206
RM254
6
6
6
6
6
6
6
11
11
11
11
11
11
Result
RM597
RM589
RM190
RM587
waxy
RM204
RM225
Trait
Primer
Amylose content
RM597
RM589
RM190
RM587
Waxy
RM204
RM225
Chromosome Mono/Polymorphism
6
6
6
6
6
6
6
Monomorphic
polymorphic
Monomorphic
polymorphic
Monomorphic
polymorphic
polymorphic
Size (bp)
CAR3 PRD
160
230
150
145
150
190
160
160
Result
RM111
RM50
RM121
PSS-II-1
PSS-II-2
RM136
GT11
Gelatinization Temperature RM111
RM50
RM121
PSS-II-1
PSS-II-2
RM136
GT11
6
6
6
6
6
6
6
Monomorphic
polymorphic
Monomorphic
Monomorphic
polymorphic
polymorphic
Monomorphic
210
100
-
200
108
-
Result
RM229
RM457
RM21
RM473E
RM206
RM254
Gelatinization Temperature RM229
RM457
RM21
RM473E
RM206
RM254
11
11
11
11
11
11
polymorphic
Monomorphic
Monomorphic
polymorphic
polymorphic
polymorphic
125
100
130
165
118
110
165
167
Result
Chromosome 8
RG1
BADH aroma
RG28
Trait
Primer Chromosome
Mono/Polymorphism
Aroma
BADH
8
Size (bp)
CAR3
PRD
polymorphic 410
400
Discussion
Trait
Primer
Amylose content
RM597
RM589
RM190
RM587
Waxy
RM204
RM225
Chromosome Mono/Polymorphism
6
6
6
6
6
6
6
Trait
Amylose content (%)
Gel consistency (mm)
Monomorphic
polymorphic
Monomorphic
polymorphic
Monomorphic
polymorphic
polymorphic
CAR3
21.3
60
Size (bp)
CAR3 PRD
160
230
150
145
PRD
23.4
81
150
190
160
160
Discussion
Gelatinization Temperature RM111
RM50
RM121
PSS-II-1
PSS-II-2
RM136
GT11
6
6
6
6
6
6
6
Monomorphic
polymorphic
Monomorphic
Monomorphic
polymorphic
polymorphic
Monomorphic
210
100
-
200
108
-
Gelatinization Temperature RM229
RM457
RM21
RM473E
RM206
RM254
11
11
11
11
11
11
polymorphic
Monomorphic
Monomorphic
polymorphic
polymorphic
polymorphic
125
100
130
165
118
110
165
167
Trait
CAR3
Gelatinization Temperature Low/intermidiat
PRD
Low
Discussion
Trait
Primer Chromosome
Mono/Polymorphism
Aroma
BADH
Trait
Aroma
8
Size (bp)
CAR3
PRD
polymorphic 410
CAR3
Non aroma
400
PRD
Aroma
Acknowledgment
• We would like to express gratitude to
Dr. Theeyarut Toojinda
• RGDU
• All staff
• Ms. Jona for her good guidance's
Thanks for your
attention!!!