Transcript Wednesday, September 5

LAB ONE
Diffusion and Osmosis
LAB ONE
Diffusion and Osmosis
Background Concepts:
• diffusion & osmosis
• selectively permeable membranes
• water potential
• molarity
LAB ONE
Diffusion and Osmosis
After doing this lab, you should be able to:
LAB ONE
Diffusion and Osmosis
After doing this lab, you should be able to:
• Measure the water potential
of a solution in a controlled
experiment.
LAB ONE
Diffusion and Osmosis
After doing this lab, you should be able to:
• Determine the osmotic
concentration of living tissue
or an unknown solution from
experimental data.
LAB ONE
Diffusion and Osmosis
After doing this lab, you should be able to:
• Describe the effects of water
gain or loss in animal and
plant cells.
LAB ONE
Diffusion and Osmosis
After doing this lab, you should be able to:
• Relate osmotic potential to
solute concentration and
water potential.
LAB ONE
Diffusion and Osmosis
EXERCISE 1A: Diffusion
.
LAB ONE
Diffusion and Osmosis
EXERCISE 1A: Diffusion
dialysis
tubing
beaker
LAB ONE
Diffusion and Osmosis
EXERCISE 1A: Diffusion
15% glucose
1% starch
dialysis
tubing
beaker
LAB ONE
Diffusion and Osmosis
EXERCISE 1A: Diffusion
15% glucose
1% starch
distilled
water
dialysis
tubing
beaker
LAB ONE
Diffusion and Osmosis
EXERCISE 1A: Diffusion
15% glucose
1% starch
distilled
water
dialysis
tubing
Iodine
(Lugol’s)
beaker
LAB ONE
Diffusion and Osmosis
EXERCISE 1A: Diffusion
LAB ONE
Diffusion and Osmosis
EXERCISE 1A: Diffusion
30 min.
LAB ONE
Diffusion and Osmosis
EXERCISE 1A: Diffusion
30 min.
LAB ONE
Diffusion and Osmosis
EXERCISE 1A: Diffusion
Glucose test
LAB ONE
Diffusion and Osmosis
EXERCISE 1A: Diffusion
Glucose test
positive
LAB ONE
Diffusion and Osmosis
EXERCISE 1A: Diffusion
Glucose test
positive
positive
LAB ONE
Diffusion and Osmosis
EXERCISE 1B: Osmosis
dialysis bags
LAB ONE
Diffusion and Osmosis
EXERCISE 1B: Osmosis
1.0 M
0.8 M
0.6 M
0.4 M
0.2 M
distilled
sucrose solutions of
various molarities
LAB ONE
Diffusion and Osmosis
EXERCISE 1B: Osmosis
1.0 M
0.8 M
0.6 M
0.4 M
0.2 M
distilled
26.0 g
26.2 g
26.1 g
26.4 g
26.3 g
26.4 g
LAB ONE
Diffusion and Osmosis
EXERCISE 1B: Osmosis
distilled
water
26.0 g
26.2 g
26.1 g
26.4 g
26.3 g
26.4 g
LAB ONE
Diffusion and Osmosis
EXERCISE 1B: Osmosis
30 min.
26.0 g
26.2 g
26.1 g
26.4 g
26.3 g
26.4 g
LAB ONE
Diffusion and Osmosis
EXERCISE 1B: Osmosis
1.0 M
0.8 M
0.6 M
0.4 M
0.2 M
distilled
gently blot dry
26.0 g
26.2 g
26.1 g
26.4 g
26.3 g
26.4 g
LAB ONE
Diffusion and Osmosis
EXERCISE 1B: Osmosis
1.0 M
0.8 M
0.6 M
0.4 M
0.2 M
distilled
26.0 g
26.2 g
26.1 g
26.4 g
26.3 g
26.4 g
26.3 g
27.0 g
28.1 g
29.3 g
30.2 g
31.2 g
LAB ONE
Diffusion and Osmosis
EXERCISE 1C: Water Potential
LAB ONE
Diffusion and Osmosis
EXERCISE 1C: Water Potential
potato
LAB ONE
Diffusion and Osmosis
EXERCISE 1C: Water Potential
LAB ONE
Diffusion and Osmosis
EXERCISE 1C: Water Potential
potato cubes
LAB ONE
Diffusion and Osmosis
EXERCISE 1B: Osmosis
0.4 M
distilled
0.2 M
0.8 M
0.6 M
1.0 M
sucrose solutions of
various molarities
LAB ONE
Diffusion and Osmosis
EXERCISE 1B: Osmosis
LAB ONE
Diffusion and Osmosis
EXERCISE 1B: Osmosis
overnight
LAB ONE
Diffusion and Osmosis
EXERCISE 1B: Osmosis
overnight
LAB ONE
Diffusion and Osmosis
EXERCISE 1B: Osmosis
overnight
overnight
LAB ONE
Diffusion and Osmosis
EXERCISE 1B: Osmosis
overnight
overnight
LAB ONE
Diffusion and Osmosis
EXERCISE 1B: Osmosis
overnight
overnight
LAB ONE
Diffusion and Osmosis
EXERCISE 1B: Osmosis
LAB ONE
Diffusion and Osmosis
EXERCISE 1E: Plasmolysis
MATERIALS
•
•
•
•
An onion
A microscope
15 % NaCl solution (a few drops)
Distilled water.
LAB ONE
Diffusion and Osmosis
EXERCISE 1E: Plasmolysis
PROCEDURE
• Observe onion cells @ 100x
• Flood with NaCl and observe
• Flood with distilled H2O and
observe.