Transcript Synthesis and Magnetic Multiplex Separation of

Houston Museum of Natural Science
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Ultrasmall (Nano)
Magnetic Separation
Cafer T. Yavuz
[email protected]
Chemistry Department
Rice University
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Nano Meter (nm) ?
100,000,000 m
Earth from space
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Nano Meter (nm) ?
10,000,000 m
100,000,000 m
North America
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Nano Meter (nm) ?
1,000,000 m
10,000,000 m
100,000,000 m
California
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Nano Meter (nm) ?
100,000 m
1,000,000 m
10,000,000 m
100,000,000 m
San Francisco Bay Area
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Nano Meter (nm) ?
10,000 m
100,000 m
1,000,000 m
10,000,000 m
100,000,000 m
San Francisco Downtown
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Nano Meter (nm) ?
1,000 m
10,000 m
100,000 m
1,000,000 m
10,000,000 m
100,000,000 m
Golden Gate Park
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Nano Meter (nm) ?
100 m
1,000 m
10,000 m
100,000 m
1,000,000 m
10,000,000 m
100,000,000 m
Japanese Tea Garden
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Nano Meter (nm) ?
10 m
100 m
1,000 m
10,000 m
100,000 m
1,000,000 m
10,000,000 m
100,000,000 m
A pond with lily pads
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Nano Meter (nm) ?
1m
10 m
100 m
1,000 m
1m
10,000 m
100,000 m
1,000,000 m
10,000,000 m
100,000,000 m
The 1 meter square
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Nano Meter (nm) ?
10 cm
0.1 m
A lily and a bee
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Nano Meter (nm) ?
1 cm
0.01 m
0.1 m
A bee’s head
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Nano Meter (nm) ?
1 mm
0.001 m
0.01 m
0.1 m
A bee’s eye
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Nano Meter (nm) ?
10 μm
0.0001 m
0.001 m
0.01 m
0.1 m
A pollen on the eye
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Nano Meter (nm) ?
100 μm
0.00001 m
0.0001 m
0.001 m
0.01 m
0.1 m
Bacteria on the pollen
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Nano Meter (nm) ?
0.000001 m
1000 nm
0.00001 m
0.0001 m
0.001 m
0.01 m
0.1 m
Virus on a bacterium
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Nano Meter (nm) ?
0.0000001 m
0.000001 m
100 nm
0.00001 m
0.0001 m
0.001 m
0.01 m
0.1 m
A Virus
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Nano Meter (nm) ?
0.00000001 m
0.0000001 m
0.000001 m
10 nm
0.00001 m
0.0001 m
0.001 m
0.01 m
0.1 m
DNA within a Virus
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1 nm = 1 Nano Meter
0.000000001 m
0.00000001 m
0.0000001 m
0.000001 m
1 nm
0.00001 m
0.0001 m
0.001 m
0.01 m
0.1 m
The molecules of DNA
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What nano brings to us?
bulk
nano
AIDS Diagnosis Chip
 High Surface area
 Smaller instruments
 Better Catalysts
 More Efficient Toxic Waste Removal
 Recoverability
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Magnetic Material
Fe3O4
Magnetite
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Very Small Iron Oxide Nanocrystals
100 nm
100 nm
26.88 nm
13.96 nm
50 nm
11.72 nm
20 nm
3.95 nm
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What we made were magnetic!
http://nanonet.rice.edu/personal/cafer/vicki/Magnetite_is_magnetic.MOV
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Magnetic Separation?
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Nanosize Magnetic Separation Setup
Magnetite (Fe3O4) Nano Crystals
Electro Magnets
Clean Effluent
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Let’s Try it!
4 nm and 12 nm mixed
High Magnetic Field
4 nm
(95%)
fraction
Low Magnetic Field
12 nm
(99.5%)
fraction
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Arsenic (As) Removal from Water Supplies
VERY TOXIC
As (V)
As (III)
200000
200000
175000
150000
11.72 nm
20 nm
100000
300 nm
50000
125000
q (mg/kg)
q (mg/kg)
150000
11.72 nm
100000
20 nm
75000
300 nm
50000
25000
0
0
0
2
4
6
8
10
12
14
16
18
20
0
5
C (mg/L)
10
15
25
20
C (mg/L)
 High surface area
 Recyclable material
* Data acquired by Dr. Tomson group at Rice University
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What’s next?: Protein Separation
A
B
C
D
+
One step
A
B
Magnetic Multiplex Separation
C
D
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Conclusion
Nanosize Magnetite is whole lot better than bulk,
Because:
•
•
•
•
They hang in the solution!
More surface area
Different Magnetic Properties
Recoverable!
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Thanks for your attention
Dr. Vicki L. Colvin
J. T. Mayo
Dr. William Yu
Joshua Falkner
Dr. Amy Kan
Dr. Mason Tomson
Dr. Lili Cong
Su Jin Yean
Center for
Biological and
Environmental
Nanotechnology
Funded and supported by,
National Science Foundation
Center for Biological and Environmental Nanotechnology (CBEN)
http://CBEN.rice.edu
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