Transcript PowerPoint-presentatie

TU/e
Master of Applied Physics
Track (specialisatie):
Physical Instrumentation/Medical Physics
• Ontwikkeling van fysische meetmethoden en
-instrumenten voor laboratoria, industrie en
biomedische toepassingen
Track info 23-3-2009
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TU/e
Physical Instrumentation/Medical Physics
TPM
MBx
Transport in Permeable Media
Molecular Biosensors for medical diagnostics
Medical Physics (Klinische Fysica)
Track info 23-3-2009
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TU/e
TPM
Transport in Permeable Media
• Meettechniek (vooral op NMR/MRI gebaseerd)
• Vocht- en ionentransport in permeabele
media, inclusief diffusie-processen op
microscopische en macroscopische schaal
Track info 23-3-2009
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Permeabele Media
• Poreuze materialen




Bouwmaterialen
Geologische gesteenten, zand, klei
Kool, hout, gel
Chemische reactoren
• (Kunstmatige) membranen, coatings
• Biologische of bio-compatibele weefsels
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moisture
hygric expansion
+ ions
fire
freezing
boiling
drying
crystallization
parameters
<- damp water ->
permeability
phase
transition
crystal
mechanical stress
fracture
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Invloed magnetische verontreinigingen (vooral Fe)
Korte T2, snel NMR signaalverval
SEM
EPMA
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NMR/MRI scanners
0.7 Tesla
1.5 Tesla
4.7 Tesla
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Relaxatie en diffusie: porie-structuur
t2
bulk water
10000
t1
pore
wall
signal (a.u.)
pore
fluid
1000
water in mortar
100
noise level
10
0
1
2
3
4
5
6
7
8
9
10
11
12
time (ms)
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Verdeling vocht over poriën in mortel
1.0
Total
gel
capillary
Signal (a.u.)
0.8
0.6
0.4
0.2
0
0.0
0
20
40
60
10
80
20
drying time (hours)
30
)
rs
40
50
60
1
(
dr
70
10
pore size (nm)
Track info 23-3-2009
g
yin
e
tim
u
ho
100
80
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Cryoporometry + NMR relaxation
10
T2
(ms)
large
pores
1
V
T2 
S
small
pores
0.1
-30
-20
-10
T (°C)
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0
10
4 clTm
Tm 
ac  H f
pore
radius
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Schade door zout-kristallisatie (Na2CO3)
2 cl cos
Pc 
rp
4
Θ
crystal
pore
RT  C 
Pc 
ln 
vc  C0 
Concentration (molal)
r
5
3
P = 10.5 Mpa
2
5 nm
10 nm
12 nm
bulk
P = 7.7 Mpa
1
0
0
10
20
30
40
50
temperature (°C)
bulk
PRL 94, 075503 (2005)
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Top view
Paint
Dun laagje verf
Evaporation
Side view
NMR visible
NMR invisible
Paint
~ 200 µm
Cover glass
~ 100 µm
Sensitive region
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Signal Intensity (a.u.)
High resolution MRI of coatings
30
field gradient 36 T/m
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depth resolution  6 μm
Evaporation
&
shrinkage
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Hardening
15
10
5
0
0
20
40
60
80
100
120
140
Position (µm)
Mobility decrease due to
cross-linking yields a faster
NMR signal decay
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High resolution MRI of coatings
10000
Air
Air
2
2
2]
(x
[μm
(x-- x
x00))2[µm]
8000
Argon
Argon
Air
Air
curing process
is limited by
oxygen diffusion
6000
4000
2000
0
0
20
40
60
80
100
(tt -- tt00[h]
) [h]
f (t )  f 0  2D0 (t  t0 )
APL 86, 134105 (2005)
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front position
oxygen density at the surface
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Medical Physics / Klinische Fysica
• Vakgebied waar de natuurkundige samen met
medische specialisten in een ziekenhuis werkt aan
de patiëntenzorg
• Stage- en afstudeerplaatsen binnen ziekenhuizen
en de medische industrie
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Diffusie- en relaxatie-gewogen hersenscans
diffusie
T2
T.g.v. een infarct is de structuur van het weefsel veranderd
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Fiber tracking
tibialis anterior muscle
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Fiber tracking resultaten
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Diffusie gewogen
NMR/MRI
Neonate with brain injury
fiber tracking results
0 months
3 months
Rat hippocampus
verification of brain
damage model
Track info 23-3-2009
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Stage- en afstudeerprojecten
theorie
modelvorming
constructie
tests
Informatie:
Track info 23-3-2009
meten
interpreteren
prof.dr.ir. Klaas Kopinga (TPM/KF)
prof.dr.ir. Pieter Wijn (KF)
dr.ir. Leo Pel (TPM)
dr.ir. Henk Huinink (TPM)
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Molecular Biosensors for medical diagnostics (MBx)
Research on new sensor principles for the in vitro detection of both
concentration and function of molecules in biological samples
10-12 -10-15 M (!)
requires new detection schemes
proteins are messengers
in biological systems
with low background
 biomarkers
give insight in health / disease
probe molecular
binding strength
- relates to affinity
- key to biological function
Biosensors: a system challenge
use
biological
recognition
toolbox of
physics
signal
immuno-assay with
or without labels
Group investigates magnetic particle labels:
low biological background and possibility for particle manipulation
Research topics:
- optical and magnetic detection techniques
- particle manipulation with
magnetic fields
microfluidic flow
probing affinities in
biochemical systems
example: on-chip manipulation and characterization of magnetic particles
- properties of superparamagnetic particles from Brownian motion
in a magnetic potential well
van Ommering et al APL 89 (2006) 142511
optical particle
tracking
current wire
magnetic
potential
particle distributions
 magnetic susceptibility
example: affinity probing by magnetic forces
Setup for pulling
(home build,
external magnet)
Microscope
Sample Holder
Force up to 100 pN
Magnetic system
Force uniform within 2%
over 150 m x 150 m
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antibody from immune system
protein
Experiments on model system: Anti-Biotin - biotinylated-BSA
constant force
Fm
quantification
work in progress
MBx students usually:
- work at the interface of physics and biochemistry
- apply sensitive optical detection techniques
- develop and realize ideas to manipulate magnetic particles
- develop quantitative models to describe
biochemical processes
For information:
dr. Leo van IJzendoorn (N-laag f 1.06)
dr. ir. Arthur de Jong (N-laag f 1.04)
TU/e
PROFIELVAKKEN
blok E
3F190
Clinical MRI
Wijn
E+F
3
3F200
Medical physics
Wijn
*
3
3F210
Safety and risk analysis
Van Kleffens
E+F
3
3F220
Radiation protection and radiation
dosimetry
Huyskens
E+F
3
3F230
Measuring and monitoring of vital
functions
Wijn
A+B
3
3F240
NMR/MRI
Kopinga/Strijkers
B+C
3
3F250
Transport in porous media
Pel
E+F
3
3S100
Magnetism and Magnetic Materials
Swagten
A+B
3
3S300
Spintronics and nanomagnetism
Koopmans
E+F
3
3S250
Molecular Photophysics
Janssen/Meskers
A+B
3
Track info 23-3-2009
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TU/e
PROFIELVAKKEN
blok E
3S170
Introduction Nanotechnology
Koenraad
A+B
3
3S190
Biosensors for medical diagnostics
Van IJzendoorn
E+F
3
8H010
New developments in fluorescence
microscopy on living tissues
Slaaf
B+C
3
8N010
Physiological NMR
Nicolay
D
3
8S070
Clinical Chemistry
Vader
A+B
3
8S080
Protein engineering
Merkx
B+C
3
8W090
Cardiovascular fluid mechanics
Van de Vosse
D
3
8W120
Cell mechanics
Bouten
?
3
8W150
Multifluid dynamics
Van de Vosse
E+F
3
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