Transcript PRODUK DNA REKOMBINAN

PRODUK DNA REKOMBINAN
Agustina Setiawati
Prinsip DNA rekombinan
STRATEGI KLONING GENA EUKARIOT
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Isolasi total mRNA dari jaringan ttt
Ubah mRNA menjadi cDNA
Sisipkan pada vektor ekspresi
Transformasi
Isolasi protein produk
PROTEIN FARMASETIK HASIL REKOMBINAN
Produksi Insulin
Proinsulin
Berg JM, et al, 2002
TRANSGENIC
PLANT
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EDIBLE VACCINE
VIRUS-RESISTENT PLANT
EDIBLE VACCINE
Edible vaccines are vaccines produced in plants that can be
administered directly through the ingestion of plant materials
containing the vaccine. Eating the plant would then confer immunity
against diseases.
Edible vaccines produced by transgenic plants are
attractive for many reasons. The cost associated
with the production of the vaccine is low,
especially since the vaccine can be ingested
directly, and vaccine production can be rapidly up
scaled should the need arises. Edible vaccine is
likely to reach more individuals in developing
countries.
The first human clinical trial took place in 1997.
Vaccine against the toxin from the bacteria E.coli
was produced in potato. Ingestion of this
transgenic potato resulted in satisfactory
vaccinations and no adverse effects.
What exactly are
“edible vaccines?”
• Biopharmaceuticals
• Plants or crops that produce human vaccines
• The next generation of vaccines
ALUR PEMBUATAN EDDIBLE VACCINE
VIRUS RESISTANT PLANT
Transgenic Animals
SOMATIC NUCLEAR CELL TRANSFER
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Transgenic animal was
constructed based on SNCT
by Robert et al (1952)
DOLLY SHEEP, first succeed cloning
Uses for transgenic animals
Gene pharming
Food/Feed
Xenotransplantation
Industrial
Transgenic Animal
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The foreign gene is constructed using recombinant
DNA technology.
In addition to a structural gene, the DNA usually
includes other sequences to enable it to be
incorporated into the DNA of the host, and to be
expressed correctly by the cells of the host.
Recombinant protein production in the milk of
a transgenic sheep
GENE THERAPY
Agustina Setiawati, M.Sc., Apt
DEFINITION
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Gene therapy is a technique for correcting “defective”
genes responsible for disease development.
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