Transcript Frozen Vials - MAPA (Mid-Atlantic Pathologists' Assistants)

Frozen Vials
Normal & abnormal vials can be
designated as such w/different
colored caps or by manipulating
barcodes.
Paraffin Sections
• A section of tissue is placed in a cassette
and fixed in formalin to create a paraffin
block.
• Origin = directly adjacent to frozen section
taken from same specimen
– “Matched samples”
• Stained (H&E) and Unstained Slides
– Quality assurance
– Diagnosis confirmation
– Photography
• Archived paraffin retrieval
– 10-15 years
• Can also create paraffin curls (20μm)
TMAs
• Tissue Microarray
• Many samples, one marker
• Lower cost to researchers
than gene microarrays.
OCTs
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Tissue placed in small mold
Embedded in Optimum Cutting Temperature (OCT) compound
Once frozen, cube (“chiclets”) is transferred to an air-tight Cryosette ® to prevent
dehydration during storage.
Label Cryosette ®
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Pt. representative ID
Sample ID
Generally cut to
match paraffin and
frozen sections.
Fresh Tissue
• Allocate & weigh tissue
• Place in correct (pre-marked) vials
or self-label tubes
• Transport media
– Researcher-provided
– RPMI w/antibiotic
– PBS
• Record ischemic time, specimen ID,
sample ID, weights, etc.
• Package
• Notify researcher/send
documentation
• Pick-up
Blood, Blood Products & Other Fluids
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Whole Blood
Serum
Plasma
Synovial Fluid
Aspirates
Smears
Tissue Banking Operations
– Sample Data:
• Sample ID
• Weight of Sample (frozen tissue)
• Ischemic time
– Case Data:
• Case ID
• Top line diagnosis
• De-identified Pathology report
– Pathology Verification
• Tumor vs Lesion vs Normal
• Percentage of tumor (when present)
Data Tracking w/Excel
Tissue Banking Operations
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Inventory management
Need to develop both physical and database storage systems
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A system for all tissue formats
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(Blocks, slides, OCT samples, frozen samples, blood and serum samples)
Storing options
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Labeling
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Cold storage vs. ambient temperature
Storage boxes
Sample vials
Cryosetts or Mega-cassettes
Blood samples
Label printers
Label designed for -180 C
Labeling conventions
Bar coding technology
Freezer
Storage Temperature: (-80) C
Capacity:
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15360 1.2 ml vials
5400 OCT Cryosetts
Inventory
• Tracking Sheets
• Bar-coding
– Self vs. pre-labeled
– Scanner
• Excel Spreadsheet
– Data transfer
• Storage Considerations
– Cold Temp.
– Ambient Temp.
Tissue Banking Operations
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Inventory management
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Develop a database for all samples in the inventory
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Excel or Access databases (home grown systems)
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Dedicated tissue banking software programs are available
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caBig (NCI)
Gulfstream
Daedalus
Freezer works “free versions” available
Samples need to be both checked in and checked out when withdrawn
from inventory
Inventory List
Tissue Banking Operations
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Data Management
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Types of pathology and clinical data
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Medical records
Pathology reports
Patient interviews
Internal management of pathology reports
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What to extract from pathology reports to include with tissue sample
De-identification of pathology reports including names, dates, and case numbers (Software is
available for this task).
Clinical Data for Clients
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IRB approval of clinical data forms supplied by client.
Extracting information from medical charts vs. patient interviews
Tissue Banking Operations
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Data Management
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Storage of clinical information and Pathology Data
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Commercial systems available
Pathology Information Systems
All information should be based on searchable and retrievable data points
(not free text)
Operations
• Equipment needs
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Revco (-80 degree Celsius freezer)
Scale (to at least 100 mg)
Label printer with labels that can withstand -180
Source / storage of liquid nitrogen (Cryoshipper)
Computer / Printer
Operations
• Personnel requirements / Staffing
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What is the scope of the Tissue Banking Program
One man show vs. a full scale operation
What support can come for other departments
Is the tissue bank supporting both internal research,
external research or both
– Capacity
Operations
• Financial considerations
– Funding possibilities
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Hospital commitment to the effort
Pharmaceutical collaborations
Tissue brokers collaborations
Internal research grants / Department support
Academic collaborations
National grants
“Tasks”
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Determine what case types to bank
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Review surgical schedule to identify desirable cases
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Meeting and consenting patients
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Communication with the OR to identify targeted case and to arrange for timely specimen
transport
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Handling the tissue/blood (Dividing and processing for various sample formats)
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Labeling samples and placing them into an inventory
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Maintaining the inventory (Update deposits and withdraws)
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Processing of histology blocks
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Pathology verification (tumor percent, ischemic time, sample weight)
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Develop / purchase a searchable database that can hold case information
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Data collection (Pathology report and clinical data retrieval)
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De-identification of all patient demographics
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Shipping or transporting samples to the researcher
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Office responsibilities
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Billing management for both external accounts.
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Equipment and supply needs
Thanks!
Contact Information
• Mike Jones, MD Principal Investigator
[email protected] 207-662-2959
• Paul Auclair, DMD
– [email protected]
207-662-4025
• Rocky Ackroyd, Path Ass’t
– [email protected]
207-662-6825
Web Site: www.mmctissuebank.org