Transcript PowerPoint template - InterAcademy Panel

Advanced Confocal Microendoscopy
Wibool Piyawattanametha, Ph.D.
High resolution subsurface imaging
RESOLUTION (log)
1 mm
ULTRASOUND
MRI, CT
100 mm
10 mm
OPTICAL COHERENCE
TOMOGRAPHY
DUAL-AXES
CONFOCAL
MICROSCOPY
OPTICAL
COHERENCE
MICROSCOPY
1 mm
CONFOCAL
MICROSCOPY
0
TWO-PHOTON
MICROSCOPY
100 mm
1 mm
1 cm
IMAGE PENETRATION (log)
10 cm
What are we trying to achieve?
Provide optical sectioning with histopathology
resolution with molecular contrast in real time
Benefits:
• Cancer screening (sub-surface imaging):
- Early detection of cancer
- Aid tumor staging diagnosis
• Locate tumor margin/boundary
• Track targeted therapy
• etc.
“Virtual biopsy” with miniature
microendoscope
Tabletop
Zeiss®
Handheld
1m
miniaturized
1 cm
Cancers of epithelium
• Malignant tissues originating from epithelial
layer of organs
- Epithelial layer: 0-700 µm from the tissue surface
• Extremely common cancer:
- 85% of all new cancer cases
• Extremely deadly:
- 80% of all cancer deaths
• Examples: ovary, cervix, upper and lower GI, ENT,
brain, bladder, etc.
Confocal microscope & endoscope
10-mm
5-mm
1 cm
5 cm
10-mm intravital & skin scope (785-nm)
5-mm GI endoscope (785-nm)
Microelectromechanical systems (MEMS)
based microendoscope (5-mm diameter)
4 mm
MEMS scanner
Assembly of a 5-mm diameter microendoscope
Ex vivo imaging of human colon
Normal
Crypt
lumens
Colonocyte
Adenomatous polyps
Scale bars: 100 µm
Normal squamous esophagus biopsy
Vertical Section
Horizontal Section (275 mm deep)
Vertical Section
500
mm
300
mm
800 mm
800 mm
Barrett’s esophagus biopsy
Horizontal Section (200 mm deep)
Vertical Section
350
mm
500
mm
800 mm
800 mm
Real-time in vivo tumor vasculature
100 µm
Mosaicing of real-time in vivo human skin
Real-time in vivo imaging of human colon
[email protected]