Transcript Background - Harvard University

Background
Demographic:
 Undergraduate Students in Pre-Lab
Project:
 Streaming On-line Biology Video pieces: One per Preceptor, per year.
Individual Video Breakdown:
 Intro: Host, Dr. Robert Lue
 Faculty Spotlight: Harvard Professor in the particular field of lab and study
 Lab/Pre-Lab: Actual Hands On Demonstration of Lab of Topic
 Outro: Host, Dr. Robert Lue
 Mandatory Feed Back Form (Quiz)
Browser/Monitor:
 Mac/PC; Quicktime/Flash
Executive Producer:
 Robert Lue 5-9924
“Players”
Associate Producer:
 Alain Viel 6-5606
Director of Photography/
Production Manager:
 Leigh Stimolo 6-7093
Content Supervisor/Coordinator:
 Christine Rodriguez 203-665-6434
Writers:
 Cheryl Vaughan 401-383-5968
 Christine Rodriguez 203-665-6434
 Tom Torello 6-1206
 Erica Pantages 4-7925
 Marion Shonn TBA
On-Location Audio Engineer:
 Jack Howard 5-4035
Videographers/Editors:
 Lansing Wagner 5-2683
 Steve Keirstead 5-2683
 Leigh Stimolo 6-7093
Production Assistants:
 Eliza Stephens 5-8258
 Clark Magnan 5-8258
Voice Over Studio/Engineer:
 Bill Countie, Media Production Center 5-9440
Animator:
 Matt Bohan, Freelance Flash Animator
A/V Multimedia Developer:
 Leigh Stimolo 6-7093
Video Server Network Administrators:
 Webmaster: Steve Keirstead 5-2683
 Chiasm Server: Pepe Lopez 6-8479
Pre-production Deliverables
Choose Lab Topic
Create Outline
Book Talent
Storyboard Lab Video Sequence
Book Crew
Script Lab Video Sequence
Recruit Production Assistants
Begin Location Scouting
Finalized Script with Shot Sequence
Research and Submit Talent Selection
1-2 Day Lab Shoot
Script Rewrite
Collect Signed Talent Release Forms
Talent Rehearsals
Collect Master Tapes & Label
Shot List Breakdown
Hand off Master Lab Video Tapes to Editor
Book Location
Select Faculty member for Spotlight
Pre-production Deliverables
Compose Ten Faculty Spotlight Questions
Submit Questions
Optional Presence at Faculty Spotlight Shoot
Compose a small 1-2 paragraph Intro and Outro script
Compose Evaluation Questions for Quiz
Production Deliverables
Lab Video Sequence Shoot—
Faculty Spotlight Shoot—
Intro/Outro Shoot—
Audio: Jack Howard
Audio: Jack Howard
Audio: Jack Howard
Video: Steve Keirstead (BS 54)
and Lansing Wagner (BS 50/BS 52)
Video: Leigh Stimolo
Video: Leigh Stimolo
Lighting: Leigh Stimolo
Lighting: Leigh Stimolo
Lighting: Leigh Stimolo
Interviewer: Robert Lue
Post-Production Deliverables
Animation Sequences—
Lab Video Sequence Editing—
Animation Script
Video Editors: Lansing Wagner (BS 50
& BS 52) and Steve Keirstead (BS 54)
Animation Storyboard
Line Producer: Leigh Stimolo
Animator: Matt Bohan
On-line Compressionist: Leigh Stimolo
Voice Over (VO)—
User Interface—
Voice Over Script
Developer: Leigh Stimolo
VO Studio: Bill Countie,
Media Production Center
Web site—
Developer/Programmer: Leigh Stimolo
BS 50: Topics
BS50
 Sterile techniques (basics, plating microorganisms, titers and dilutions, mammalian cell
culture)
 Chip analysis (techniques, SNPs/pharmacogenomics, transcriptional profiling,
limitations of data)
 Meiosis and tetrad analysis (Meiosis animation, Neurospora vs Saccharomyces, tetrad
dissection vs random sporulation)
 Use of reporters (B-galactosidase, GFP, others)
 Northerns and Southerns
 Cloning, transformation, and transfection
BS 52: Topics
BS 52
 Electrophoresis (agarose vs acrylamide, what %, what Amp/Volt to run at, IEF, capillary)
 DNA sequencing (Restriction enzyme mapping, lab/gel techniques, methods such as
PCR, Sanger, automated, etc.)
 DNA topology (1D/2D gels, animation of enzymatic reaction, run on gel)
 Gel shift/analysis of DNA binding proteins
 Staining and labeling (histology/cells, DNA, types of stains used, when and why they
work, radioactivity, enzymatic assays)
 FISH
http://www.courses.fas.harvard.edu/~bs52
BS 54: Topics
BS54
 Centrifugation (vocabulary, techniques, types of rotors, supernatant vs pellet, from
microfuge to Sorvall to ultra)
 Generation of antibodies (polyclonal vs monoclonal, modified secondary antibodies)
 Beads and Tags (biochemical sorting, IP/Co-IPs, GST, 6-His, cell sorting, agarose
beads, etc.)
 Gradients (refer to ultracentrifugation, subcellular fractionation, how to get molecular
materials, labelled organelles?, DNA/CsCl gradient, proteins such as ribosomes, etc)
 Protein sequencing and analysis (old vs new methods)
 ELISA
http://www.courses.fas.harvard.edu/~bs54
BS 50: Year 1
BS50 : Preceptor, Christine Rodriguez (Rich Dearborn, Adam Fagen)
 MICROARRAY, Chip Analysis
On-Screen Pre-Lab Talent:
 Claire Bailey
Voice Over Talent:
 Claire Bailey (NYR)
Faculty Spotlight:
 TBD, possibles: Michael Laub, Hans Hoffman (NYR)
Other Content and Production Assistants:
 Undergraduate Student: Joo Hee & Allison
BS 52: Year 1
BS52 : Preceptor, Kenna Mills (Lauraine Dalton)
 DNA SEQUENCING
On-Screen Pre-Lab Talent:
 Dion Dickman
Voice Over Talent:
 Jan or Dion Dickman (NYR)
Faculty Spotlight:
 Dan Branton
Other Content and Production Assistants:
 Undergraduate Student: N/A
BS 54: Year 1
BS54: Preceptor, Cheryl Vaughan
 ANALYZING PROTEINS
On-Screen Pre-Lab Talent:
 John Neven, Emy Chen, Darcy Mootz
Voice Over Talent:
 Darcy Mootz
Faculty Spotlight:
 TBD, possibles: Steve Gygi (NYR)
Other Content and Production Assistants:
 Undergraduate Student: N/A
BS 50: Year 2
BS50 : Preceptor, Christine Rodriguez (Erica Pantages)
 NORTHERN/SOUTHERN, Cloning
On-Screen Pre-Lab Talent:
 TBA (NYR)
Voice Over Talent:
 TBA (NYR)
Faculty Spotlight:
 TBD, possibles: TBA (NYR)
Other Content and Production Assistants:
 Undergraduate Student: TBA
BS 52: Year 2
BS52 : Preceptor, Tom Torello
 TBD
On-Screen Pre-Lab Talent:
 TBA (NYR)
Voice Over Talent:
 TBA (NYR)
Faculty Spotlight:
 TBD, possibles: TBA (NYR)
Other Content and Production Assistants:
 Undergraduate Student: TBA
BS 54: Year 2
BS54: Preceptor, Cheryl Vaughan (Marion Shonn)
 ANTIBODIES
On-Screen Pre-Lab Talent:
 TBA (NYR)
Voice Over Talent:
 TBA (NYR)
Faculty Spotlight:
 TBD, possibles: TBA (NYR)
Other Content and Production Assistants:
 Undergraduate Student: TBA
YEAR TWO emails
Robert Lue
[email protected]
Leigh Stimolo
[email protected]
Jack Howard
[email protected]
Alain Viel
[email protected]
Cheryl Vaughan
[email protected]
Christine Rodriguez
[email protected]
Lansing Wagner
[email protected]
Steve Keirstead
[email protected]
Kenna Mills
[email protected]
Tom [email protected]
Erica Pantages
[email protected]
Marion Shonn
[email protected]
Eliza Stephens
[email protected]
Clark Magnan
[email protected]
Equipment: Camera/Audio
Shotgun Microphone
Fishpole
Canon XL-1S Mini DV Camcorder
FP-33 Stereo Mixer
Lavalier Microphone
Equipment: Lights
Arri: 300 Watt Fresnel
Tungsten Light
Tota Light Kit with
Umbrellas & Stands
SoftBox (Chimera)