Transcript G-Fectin - FroggaBio Inc

G-Fectin
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TM
A novel transfection reagent developed by
Genolution
Cationic lipid based
no cell splitting is required
Whole transfection can be done in 10 min
Your gene silencing in 24 hours
Lower toxicity than other reagents
Better gene silencing
For 500X transfection to one well of 24 well plate
G-Fectin has good Transfection efficacy
Remaining Vimentin %
120
100
80
HeLa
60
OCUG
40
SNU368
Hep3B
20
MDA-MB-231
0
lipo 2000 G-factin product 1 product 2 product 3 product 4
transfection reagent
Transfection assay using Vimentin siRNA final 10 nM
G-Fectin has low cytotoxicity
100
90
Cell viability %
80
70
60
50
40
30
20
10
0
HeLa
Skov3
MCF7
siHa
Lipo 2000
Pc3
MDA-MB 468
C4I
G-Fectin
All described cells were spitted in 30 % confluency in 24h
ahead of viable cell counting with treatment of lipo-plexed
RNA.
1-ul ofG-factin
Genolution siRNA
Add the reagent &
siRNA to the PBS
10 min transfection
protocol
with G-Fectin
50 ul of PBS
Incubate 10 min at RT
Add to 24 well
plate directly
Cells seed
on plate
450 ul of Suspended cells
(5 x 104 cells/ well)
Incubate 24 hrs
qPCR
siRNA Transfection assay kit
•
TM
G-Fectin Kit
– The easiest and fastest way for siRNA transfection assay test
– Just add to the cell, wait for 48hours, and count the viable cell
– The target gene of the used siRNA is essential for cell viability
– The cell toxicity and level of the gene silencing is proportional
Materials included
scramble siRNA
control siRNA (cause to cell death if transfected, for 200X assay )
siRNA dissolving buffer
G-factin 0.5ml (for 500 X transfection to 24 well)
Easy siRNA Transfection assay kit
Cell viability in
48 hour
Scramble siRNA
siRNA
target
100
90
Gene silencing
in 24 hour
level of gene %
80
70
60
50
100 pM
40
2.5 nM
30
20
10
0
mock
taregt gene
siRNA
Materials included
scramble siRNA
control siRNA (cause to cell death if transfected )
siRNA dissolving buffer
G-factin 0.5ml (for 500 X transfection to 24 well)