Transcript Lunch Seminar Quality in MA, Tecan

CAMDA 2008, Vienna
Experiment quality - the direct route to reliable data
5th December 2008
17.07.2015, p 2
2008 CAMDA, Ralph Beneke, Tecan
6-months survey: „Talk to Tecan“
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...About your workflow, quality, needs
...About your expections
• Setup
• Processing
• Detection
• Analysis
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...about news and views in the community
17.07.2015, p 3
2008 CAMDA, Ralph Beneke, Tecan
Results:
Consistency, Accuracy, Specificity, Sensitivity, Throughput, Budget
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Insufficient data quality?
• Wasting time, sample and money
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How to become successful?
• Considerations for planning effective and efficient lab routines
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What is Tecan´s expertise?
• „Automation“ of application processes in life science labs
17.07.2015, p 4
2008 CAMDA, Ralph Beneke, Tecan
Considerations
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Your vision about your science work and projects
Your networks & references
Your plan of lab infrastructure & resources
Availability of budget & funding
Design of experiment (incl. quality control, validation)
Optimization process, QC steps and measure
Workload, throughput
Data reduction, tracking and management in routine
17.07.2015, p 5
2008 CAMDA, Ralph Beneke, Tecan
High Definition Genomics or Proteomics
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What are the key principle of available platforms?
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Analytical capability
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Robustness
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Reproducibility
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Flexibility
17.07.2015, p 6
2008 CAMDA, Ralph Beneke, Tecan
Infrastructure
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Ease of use
Success/Failure rate (false positives and negatives, number of accidence)
Labor
Total process time, which steps are limiting
Maintenance
Support from manufacturer, tricks from experts
Computing and analysis power
Flexibility without loosing quality
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to expand on applications
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benefiting from assay changes (e.g. more data, better data, lower cost)
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when throughput increases
17.07.2015, p 7
2008 CAMDA, Ralph Beneke, Tecan
Purchase price of platform - or cost of ownership?
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Real cost of running assays now and next years:
• Initial and maintenance cost of infrastructure (depreciation)
• # samples (per run, per month and year)
• Cost of labor: Parallel and sequential hands on time to perform
assay, skills/training
• Failure/Success rate
• No - or faulty - data is more expensive than accurate data
• Old saying: „garbage in - garbage out“
is true from sample prep to data analysis
17.07.2015, p 8
2008 CAMDA, Ralph Beneke, Tecan
Purchasing decision (1/2)
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Learning from MAQC projects:
• Good experimental design
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Optimize first
Standardize process
Randomized block design
Quantify batch effects
• Use literature, cross-platform validations and ref.control
samples
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Robustness of platform and experimental design:
• Workflow simplicity cuts labor cost
• Automation minimizes process complexity and failure rate
(operator/protocol?)
17.07.2015, p 9
2008 CAMDA, Ralph Beneke, Tecan
Purchasing decision (2/2)
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Flexibility required - accross variety of applications:
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Maximizes cost effectiveness
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Ready for future requirements
17.07.2015, p 10
2008 CAMDA, Ralph Beneke, Tecan
Many sources of technical variations
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Sample generation - analysis:
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Optimization – SOPs?
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Sample prep, extraction and modification/amplification batches
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Reagent batches
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Array batches
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Hyb and wash process batches
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Protocol timing/scheduling/temp fluctuations
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„Day-conditions“ (ozone, temp, humidity, etc.)
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Operator batches
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Machine and/or position, maintenance effects
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Analysis tools &, process
17.07.2015, p 11
2008 CAMDA, Ralph Beneke, Tecan
Individual or combination of batch
effect can be higher than
treatment or genetic variation
effect
Please quantify!
17.07.2015, p 12
2008 CAMDA, Ralph Beneke, Tecan
Considerations about D-O-E
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Batch effects may obscure biological effects
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...by generating high error bars
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...if not adressed by proper design of experiment
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...which is always critical in high multi-variant genomic and proteomic
analysis
„Randomized block design“
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Batch effects are generated through the entire process chain from
sample prep to data extraction.
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Measure and address the effects by proper design of experiment
ANOVA
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Measure (QC), estimate and remove technical noise in mixed-model
analysis of variation
17.07.2015, p 15
2008 CAMDA, Ralph Beneke, Tecan
About Tecan
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Tecan´s technical expertise in lifescience:
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Assay-Process control
• Sample tracking
• Robotics and Storage
• Dispensing, incubation and seperation
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Optical detection technology
• Absorbance
• Fluorescence
• Luminescence
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Data management (LIMS)
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Delivery of GMP series-instruments in thousands/year
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Customization projects
• largest fully automated Forensics lab in RSA
• largest fully automted Antibody production facility in AUS
• ...
...since more than 25 years!
17.07.2015, p 16
2008 CAMDA, Ralph Beneke, Tecan
Tecan solutions
...standardizing and detecting state of the art assays
• You save money on
• Sample
• Reagent
• Labor
• by improving
• Robustness
• Sensitivity
• Throughput
17.07.2015, p 17
2008 CAMDA, Ralph Beneke, Tecan
Tecan News – Tecan Journal
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Applications published by our customers
• Interaction and reaction assays in microplates
• PCR
• Sequencing
• ELISA
• LC/MS
• Cell based assays
• Microarrays
• ...
17.07.2015, p 18
2008 CAMDA, Ralph Beneke, Tecan
Microarray process chain
Sample Logistics
and Preparation
Quantification
Hyb-wash and dry
Arrays, Cells or Beads
Array
Detection
Slides
MTP
REMP®
& Freedom EVO®
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Sample tubes
Fridges, Stores
Vacuum separation
Magnetic separation
Centrifugation
PCR, sequencing
and IVT setup &
cleanup
•NanoQuant infinite
200 series
• 260/280, Cy3/Cy5
• 16-samples
• 2µl, 1ng/µl
• upgradable
•HS Pro
•HydroFlex
Incubation, wash, dry
• DNA
• Protein
•PowerScanner
•LS Reloaded
•ArrayPro
Scanning
• Slides
• MTP, Gels, Fluidics
• Cells
Analysis of 16bit tiff
17.07.2015, p 19
2008 CAMDA, Ralph Beneke, Tecan
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NanoQuant Plate™ & Infinite® 200 NanoQuant Reader
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Most sensitive and accurate low volume absorbance detection
≤ 16 samples in parallel ≤ 30 seconds
≥ 1ng/µl NA (DNA & RNA) concentration and purity
≤ 10% CV
Labeling efficiency measurement
2 µl (single and 8-channel pipette compatible)
• 16 micro-well optical NanoQuant Plate, unlimited re-use
• 6-384 well standard microplate assay detection
• Upgradeable to fluorscence & luminescence multi-mode detection
reader
• Upgradeable to automatic assay kit injection (reporter assays)
17.07.2015, p 20
2008 CAMDA, Ralph Beneke, Tecan
March 2009: PowerScannerTM
real 2 µm pixel resolution for high and low definition scanning
All commercial 3x1 inch glass slides of any surface
1D and 2D barcode reading
CV of < 3% with autofocus, online slide motion control and LRC
Supports multi-segment area scanning
Autocalibration (internal check tool)
< 20% of lab ozone inside instrument to avoid dye degradation
≤ 48 slides, compact and handy magazine for fast and easy
autoloading
Online image display
Interactive PMT balancing and autogain
X-tended dynamic data range > 16bit
Adjustable laser power
Scattered light detection mode
17.07.2015, p 21
2008 CAMDA, Ralph Beneke, Tecan
Q2 2009:
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Hybstation quality control and calibration tool for HS ProTM
• Any day, any user
• Temperature offset control & automated re-calibration
• Liquid system-flow-through control
• External-Nitrogen flow-through control
• Lab-book report history
Thank you for your attention
When will you talk to Tecan about your lab experiences?
Direct route to reliable data