Transcript National Engineering Week and COE Spirit Week!

Dual Pretreatment Strategy for
Enhanced Biomass Hydrolysis
G. Brodeur, S. Ramakrishnan and J.C. Telotte
Department of Chemical and Biomedical Engineering
FAMU – FSU College of Engineering
1
Cellulose
Pretreatment
0
Hemicellulose
DA Treatment
48
2
Sweet Sorghum Bagasse
72
Zhang, J., et al., 2011
Sugarcane Bagasse
96
Our Work
Bagasse
72
Geddes, C.C., et al., 2010 Sugarcane
Sugarcane Bagasse
72
Yoon, L.W., et al., 2011
72
Sugarcane Bagasse
72
Rocha, G.M., et al., 2011
72
Switchgrass
72
Li, C., et al., 2010
Switchgrass
Aspen
72
Wyman, C.E., et al., 2011
Tian, S., et al., 2011
Aspen
Stalk
40
Jensen, J.R., et al., 2010
Silverstein, R.A., et al., 2007 Cotton
Corn Stover
Corn Stover
Corn Stover
Corn Stover
20
168
Our Work
Lignin
60
Schell, D.J., et al., 2003
Corn Stover: 190ᵒC; 1 min; 50
mg H2SO4/g dry biomass
 Florida Center for Renewable
Chemicals and Fuels (UF)
 Sugarcane Bagasse: 190ᵒC; 5
min; 0.5% w/w H3PO4

80
Um, B.H. et al., 2003
 National Renewable Energy
Laboratory (NREL)
100
Um, B.H. et al., 2003
Dilute Acid Treatment (Pilot Plant)
Glucan Conversion (%)
Even at the highest conversion there is still >15% of
the material that is left inaccessible
Hydrolysis Time (hr)
72
72
60
Cell
H
 N-methyl morpholine N-oxide (NMMO)
monohydrate
 𝑇𝑚 ≈ 76℃
 Treatment Conditions
 Moderate temperatures (100ᵒC)
 Short dissolution times (<3 hours)
 High solids loadings (up to 20% w/w)


Petrovan et al., J. Appl. Polym. Sci. 2001
This work reports data for 3-5% (w/w)
Regeneration with anti-solvent (water)
 Benefits:



Highly effective cellulose treatment
Reported ability to recycle over 99% of solvent
Biodegradable
 Raw feedstock will not completely dissolve
Cell
+
100
Glucan Conversion (%)

OH
80
60
40
NMMO Treated alpha-cellulose
20
0
untreated alpha-cellulose
0
20
40
60
80
100
Time (hr)
3
~98% Conversion
Corn Stover
100
~91% Conversion
Sugarcane Bagasse
100
DAWNT-CS
80
Glucan Conversion (%)
Glucan Conversion (%)
DAWNT-SB
DATCS
60
NTCS
40
CS
20
80
DATSB
60
NTSB
40
20
SB
0
0
10
20
30
40
50
Time (hr)
60
70
80
0
0
10
20
30 40 50
Time (hr)
60
70
80
--Reaches maximum conversion of DATCS 48 hours faster!!
--Nearly complete conversion after 72 hours
4
100
Glucan Conversion (%)
Corn Stover
80
DAWNT-CS 1 % (w/w) hydrolysis solids loading
60
DAWNT-CS 5 % (w/w) hydrolysis solids loading
40
20
0
0
10
20
30
40
50
60
70
Sugarcane Bagasse
80
DAWNT-SB 1 % (w/w) hydrolysis solids loading
DAWNT-SB 5 % (w/w) hydrolysis solids loading
60
40
20
0
80
Time (hr)
0
10
20
30
40
50
60
70
80
Time (hr)
100
Glucan Conversion (%)
Glucan Conversion (%)
100
80
Fresh NMMO
1st Recycle NMMO
2nd Recycle NMMO
3rd Recycle NMMO
60
40
20
0
0
10
20
30
40
50
Time (hr)
60
70
80
5