Transcript Slide 1

Effects of Jasmonic Acid and Salt Applications on Antioxydative
Enzyme Activities of the Eggplant Seedlings Cultured in vitro
Banu GÜNALP1, Özge HORASAN1, Fikret YAŞAR2, Şebnem KUŞVURAN3, Rukiye TIPIRDAMAZ4, Şebnem ELLİALTIOĞLU1
1Ankara
University, Faculty of Agriculture, Department of Horticulture, Ankara, Turkey
2Yüzüncü Yıl University, Faculty of Agriculture, Department of Horticulture, Van, Turkey
3Çankırı Karatekin University, Kızılırmak Vocational High School, Çankırı, Turkey
4Hacettepe University, Faculty of Science, Department of Biology, Ankara, Turkey
I. Introduction
Salinity is a major abiotic stress reducing the yield of a wide variety of crops all over the world. Different strategies are being employed to optimize growth under saline conditions. One of them is to produce salt tolerant
genotypes in different crops. In addition, many other attempts have been made to overcome this disorder, including exogenous application of plant growth regulators (1). Plant hormones are active members of the signal
compounds involved in the induction of plant stress responses (2).
Jasmonic acid (JA), which is naturally synthesized by plants, plays an important role as a signal molecule that induces the tolerance mechanisms under the influence of both biotic and abiotic stresses such as virus, bacteria
and fungi infections, freezing, drought, heat and heavy metals. Pretreatment with JA reduced the inhibitory effect of high salt concentration on growth and photosythesis of barley (3).
Eggplant (Solanum melongena L.), belongs to family Solanaceae, is an important crop for Turkey. According to the FAO 2009 statistics, Turkey with a yield of 297.000 tons in 30.000 hectares holds the first place in eggplant
production in Europe. Furthermore, it is known that embryo culture applications in eggplant shorten the period of time needed for a generation for nearly 2 to 2,5 months.
In this study we aimed to investigate the effects of JA individually and accompanied with salt stress on two cultivars of eggplant embryos cultured in vitro.
II. Materials and Methods
 Embryos gently isolated from the 32 days old seeds of eggplants were transplanted on hormone-free MS (4,5). Medium basically containing 2% saccharose, 0.7% agar at pH 5.7 . Also different concentrations of NaCl and
JA were applied.
 9 different applications and two cultivars of eggplant were used: cv. Mardin (M: Tolerant) and Kemer (K: Sensitive) (6)
Control
JA
A.No JA and NaCl (14 days)
B. No JA and NaCl (14 days)
Salt
C. JA 10 (10µM JA 14 days)
D. JA 20 (20µM JA 14 days)
Salt+ JA
E. NaCl control ( 100 mM NaCl 14 days)
Pre JA
F. NaCl+JA 10(100mMNaCl+10µJA 14 days)
G.NaCl+JA 20(100mMNaCl+20µJA 14 days)
H.Pre JA 10(10µM JA 4days)+NaCl (100mM NaCl 10 days)
I. Pre JA 20(20µM JA 4 days)+NaCl (100 mM NaCl 10 days)
 Embryos were incubated for 21 days at 25± 1 ⁰C and 16/8 hours of light/dark.
 After the incubation period, plantlets were harvested and germination percentage and antioxydative enzymes, SOD, CAT and APX, were determinated.
A
B
III. Results and Discussion
140
60
50
40
Kemer
30
M.Kızıltepe
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10
250
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150
Kemer
M.Kızıltepe
100
50
A
B
C
E
A
H
Applications
120
100
80
Kemer
60
M.Kızıltepe
40
20
0
0
APX aktivity(mol/min/mg F.W.)
300
SOD activityi (mol/min/mg F.w.)
CAT aktivity (mol/min/mg F.W.)
70
B
C
E
H
0
A
Applications
B
C
E
H
Applications
Antioxydative enzymes, CAT, SOD and APX, increased with salt or JA treatments compared to control plantlets.
The SOD, CAT and APX activities of the salt-tolerant Mardin variety were higher than salt-sensitive Kemer variety.
Conclusions
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Germınatıon Percentage (%)
100
80
60
Kemer
M.Kızıltepe
40
Among all applications of JA, plantlets that were pre-treated with 10 µM JA
and then transferred to the salty media showed relatively better
development than the other applications. As a result of our research, it is
possible to claim that JA was perceived as a stress factor by the seedlings
and JA applications resulted in enhanced enzyme activities. It can be even
said that some doses of JA caused higher inhibition and more devastating
effects on eggplant seedlings than salt applications did.
A
D
B
C
E
F
20
References
0
A
B
C
D
E
F
G
H
İ
Applications
Germination rate was 100% and plant growth was normal
in control application however germination rate and plant
growth were inhibited by salt or JA application.
1.Kaya, C., Tuna, A.L., Yokaş, I. 2009. The Role of Plant Hormones in Plants Under Salinity Stress. İn: Salinity
and Water Stress (Eds: Ashraf, M., Ozturk, M., Athar, H.R.), Springer Verlag. pp: 45-50.
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Growth Regu. 41: 149-158.
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of barley seedlengs to salinity stres. J Plant Growth Regul 17: 153-159.
4. Ellialtıoğlu, Ş., & Öztürk, S. 1998. Embryo culture studies in eggplant (Solanum melongena L.).
Biotechnological Industry University Cooperation Symposium (s. 33-38). Eskişehir: Anadolu University and the
BiotechnologyAssociation.
5. Murashige, T., Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures.
Physiol. Plantarum , 15: 473-497.
6. Yaşar, F., 2003, Investigation of some Antioxidative Enzymes on Eggplant Genotypes Grown under Salt
Stress in vivo and in vitro conditions . Yüzüncü Yıl University, Graduate School of Natural and Applied Sciences.
(Ph.D. Thesis, in Turkish)), Van, Turkey,125p.
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Effects of different applications for Mardin Kızıltepe
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