Web-Based Virtual and Hybrid Laboratories for Biomanufacturing Curriculum

Sonia Wallman, PhD NBC 2 [email protected]

Yakov Cherner, PhD ATeL, LLC [email protected]

Global BioManufacturing Curriculum

Introduction to Biomanufacturing Textbook

• • • • • Authored by Industry Experts 11 chapters plus introduction Currently being piloted by two Biomanufacturing classes, 37 Students total, and by 3 instructors teaching Biomanufacturing Industry interest Scheduled for release, fall 2011

Introduction to Biomanufacturing

Engineering

• Operational Excellence Marti Mathews, Biogen Idec • Facilities Brian Shingle, Centocor • Metrology Gene Kernan, HGSI • Validation Patrick Sheehy, HGSI • EH&S John Piampiano, Kodak •

Quality

Quality Assurance Cynthia Sarnoski, Frank Hallinan, Pfizer Ron Hudcosky, Merck

Production

• Upstream Processing Olga Mollin, Jane Flanagan, Lonza • Microbial Control John Hasyn, MCCC • QC Biochemistry Mary Harne, Zhenhong Lee., HGSI • Downstream Processing Paul O’Neil, Pall Euroflow • Process Development Ezster Birck Wilson

Biofuels Curriculum

Biofuels Textbook

Biofuels Lab Manual

Laboratory Manuals



Generic



Custom



CHO – tPA



Pichia pastoris - HSA



Protein is Cash

Generic Laboratory Manual

Metrology: SOPs for equipment Validation Protocols EH&S Program Quality Assurance: cGMP, SOPs, Batch Records, Equipment Logs Quality Control Microbiology SOPs Upstream Processing: E. coli recombinant for GFP, CHO recombinant for tPA Cell Culture, Pichia pastoris recombinant for HSA Cell Culture Downstream Processing: Purification of human tPA from CHO Cell Culture Purification of human serum albumin from Pichia pastoris Cell Culture

Quality Control Biochemistry

(Process Development)

NBC 2 Biomanufacturing Laboratory Manual Adopters

• • • • • • Mount Wachusett Community College (BMS is biomanufacturer partner) Minuteman Regional High School Adult Evening Program (Shire is biomanufacturer partner) Hudson Valley Community College (Regeneron is biomanufacturer partner) Great Bay Community College (Lonza and Stryker are biomanufacturer partners) Montgomery County Community College Hagerstown Community College

‘Protein is Cash’ lab manual Overview of Protein is Cash Manual

– Metrology – Transformation – Upstream processing – Downstream processing – Qualitative analysis – GMP/Food and Drug Analysis Can be found at: www.biomanufacturing.org

• • •

‘Protein is Cash’ : Adult Certificate Program Biomanufacturing Certificate Program

Derived from Protein is Cash and Global Manufacturing Curriculum 12 week course 2 hours of lectures, 6 hours of laboratory activities per week

The Virtual C

hromatography Laboratory on the Web

URL: http://ATeLearning.com/BioChrom/

Password:

hi-tec2011

(lower case)

Motivation for developing virtual biomanufacturing laboratories

: • Provide students of digital generation with visual instructions and learning tools that more relevant to their every-day habits and experience than traditional printed materials • Enhance hands-on training • Supplement and/or substitute for hands-on training (high school students, where time in the lab is limited, and e-learners) • Substitute for or enhancement of printed instructions for hands-on experiments (e.g. - textbooks and user’s manuals may not be necessary) • Shorten training time for students and employees • Facilitate just-in-time learning to enhance students’ understanding and to create transferrable skills and knowledge.

• Provide technology and tools for performance-based assessment.

Simulation-based Virtual Learning Environment



Simulations



Virtual experiments

(self-guided online assignments for students) 

Assessment tool



Embedded resources for “just-in-time” learning

(Online lessons, presentations, papers, etc.) 

Auxiliary resources

(glossary, animations …. )

Simulations vs. Virtual Experiments Simulation

  task and learner neutral, there are no restrictions on user’s actions

Includes:

• brief theory • help

Virtual experiment

 focuses on a particular task,  students have to follow guidelines

Includes:

• main simulation • specific learning objectives • scenario/assignment • step-by-step instructions for students • worksheet • assessment

optional components:

auxiliary simulations, prerequisites, excerpts from interactive lessons and technical manuals, quizzes, and other online reference resources.

Target audience:

Teachers and instructors Students

Comprehensive Learning/Teaching Environment



Traditional printing curriculum resources

(textbooks, user’s manual, worksheets, etc.) 

Hands-on laboratories



Virtual laboratories



Remote laboratories



Hybrid

(blended) labs)

laboratories

(hands-on & virtual labs, remote & virtual 

On-line curriculum resources



Auxiliary tools

(authoring tools for customization of virtual labs and online resources, communication tools (forums, blogs, e-mail, etc.) learning management tools)

LP Biologic Chromatography Lab

APPLICATION MODES: Introduction:

Goal:

Introduce equipment and to study the design and functionality of main system components.

Operation:

(auto and step-by-step):

Goal:

Demonstrate the system operation and major processes.

Virtual experiment:

Goal:

Facilitate an online experiment that exactly match experimental tasks that students face in workplace or in actual college labs.

Assessment

Goal:

Evaluate students’ knowledge and skills

Run Demo

Password:

hi-tec2011

Learning cycle

The proposed blended learning cycle recommends a five-step approach to student learning:  Use simulations and online materials to learn the theory and basic principles underlying protein purification;  Use simulations to become familiar with a liquid column chromatography system, its components and their function;   Perform virtual experiment(s);  Evaluate knowledge and virtual skills, using a built-in assessment tool, before granting access to the real chromatography system; Use the real

BioLogic ®

LC Chromatography System to purify proteins.

Future plans:

 Completing the major blocks of this Virtual Liquid Chromatography Laboratory (i.e., Lessons, Assessment, Glossary)  Developing new virtual experiments;  Creating virtual labs and relevant e-learning materials for other subjects;  Thorough testing to evaluate and compare the impact on student learning of hybrid labs versus the separate use of hands-on or virtual labs; You are invited to participate in piloting our Virtual Liquid Chromatography Lab.

URL: http://www.atelearning.com/BioChrom Password: hi-tec2011 (lower case)

We believe that the combination of online and hands-on learning ensures integration of theoretical knowledge and practical skills and enhances students’ understanding and workplace performance.

Contact

:

Dr. Yakov Cherner

ATeL, LLC Phone: (781) 842-3300 e-mail:

[email protected]

Web site

: http://ATeLearning.com

Dr. Sonia Walman

NBC 2 e-mail:

[email protected]

Web site

: http://B iomanufacturing.org