OVERVIEW R&D, Laboratories and Analysis Services

Gastien Godin General Manager Jacques Gagnon, PhD Scientific Director Fishery and Marine Coproducts Division Fredericton , NB October 8th, 2014

COASTAL ZONES RESEARCH INSTITUTE (CZRI)

• • • • LAND & PEATLANDS MANAGEMENT- AGRICULTURE & RENEWABLE RESOURCES- ADAPTATION TO CLIMATIC CHANGE AQUACULTURE FISHERY AND MARINE COPRODUCTS LABORATORIES AND ANALYSIS SERVICES • • CZRI Highlights – Value Added Food Sector Mobile Pilot Unit

2

LAND & PEATLANDS MANAGEMENT- AGRICULTURE & RENEWABLE RESOURCES ADAPTATION TO CLIMATIC CHANGE

Dr. Mathieu Quenum – Scientific Director

Email : [email protected] Tel. : (506) 336-6600     

Fields of Expertise and Strategic Research Concentration Biomass & Bioenergy Horticulture, Agriculture & Berries Sustainable Production of Sphagnum & Added Value Products Soil, Water & Environment Sustainable Development in Coastal Areas 3

AQUACULTURE

Dr. André Dumas– Scientific Director

Email : andré[email protected] Tel. : (506) 336-6600    

Fields of Expertise Nutrition of fish and crustaceans Nutrition modelling Arctic charr, salmon, oyster and lobster production Product development and innovation management

 

Research focus areas Nutritional evaluation of commodity and innovative ingredients, prebiotics, probiotics, immunostimulants Nutrigenomics 4

FISHERY AND MARINE COPRODUCTS

Dr. Jacques Gagnon– Scientific Director

Email : [email protected] Tel. : (506) 336-6600

Fields of Expertise

   

Development of green processes Lab scale spaces with hydrolysis, drying & centrifugation capacities 53’ pilot multifunctional unit for turnkey project on site Product development and innovation management Research focus areas

 

Valorization and commercialisation of marine coproducts Natural health products 5

LABORATORIES AND ANALYSIS SERVICES

 

Chemical/microbiological analyses

  Environmental, nutritional, etc.

Food safety 

ISO/IEC 17025 (CALA) R&D support

  Fatty acids & pigments Amino acids (in development)  

Personnalized services

 On-site, technical, process design, etc.

 Regulatory support, nutritional labelling, shelf life

Coproducts recovery and characterization

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Mobile Pilot Unit

• • • • • • Various tanks (2 x 1500 liters) 8 x 100 liters double walls for temperature control Various pumps Filtration unit (sweco) Mixers Etc.

• • • • 53 feet reefer trailer Processing room with temp. control Walk-in cold room and freezer 600, 220, 110 volts connections

OPEN FOR BUSINESS We look forward to new collaboration opportunities!

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Highlights – Value-Added Food Sector

• CZRI co-hosted (with AFBPA) the Sixth Acadian Peninsula Food Processing Conference

“Growing Our Industry: Making history with the new biotechnologies”

www.shippagan.ca

Acadian Peninsula Convention Centre, Shippagan, NB May 21-23, 2014

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MAXIMIZING THE VALUE OF MARINE COPRODUCTS: APPLICATIONS IN NUTRISCIENCES Jacques Gagnon, PhD and colls.

Fisheries Council of Canada 2014 Conference October 8th

Valorization of effluents, residues and marine coproducts

VALEUR.COM



VALEUR.COM = VAL

orisation des

E

ffluents d'

U

sine, des

R

ésidus et des

CO

produits

M

arins)  Full title –AIF project:

DEVELOPMENT AND COMMERCIALIZATION OF MARINE COPRODUCTS FOR USE IN ANIMAL AND HUMAN NUTRITION AND ITS APPLICATION TO THE PREVENTION OF OBESITY, DIABETES AND NEURODEGENERESCENCE

 CZRI research team: Nadia Tchoukanova Sébastien Plante and Jacques Gagnon

BioAtlantech Survey (Biosciences)

 Geographical clustering aquaculture and marine  St. George/St. Andrews (7 Companies + 2 Research Institutions)  Acadian Peninsula (7 Companies + 1 Research Institution) Now BioNB, 2013

LIST OF COPRODUCTS

SEA, PROCESSING AND AQUAFEED: A VALUE CREATION PATHWAY OF SEAFOOD RAW MATERIALS

André Dumas, PhD & colls

The Fisheries Council of Canada 2014 conference

Aquaculture in the World

100 80 60 40 20 0

2000 2010 2020 Source: FAO (2012)

Aquafeeds in the World

• • •

20 to 23 millions MT aquafeed/yr (2006) ~3.7 million MT/yr fish meal (FM) = $5.5 bln ~0,8 million MT/yr of fish oil (FO) = $1.0 bln

Source: Tacon & Metian (2008)

FM: source of beneficial nutrients

• • • •

Essential amino acids (AA) Valuable AA: Taurine, hydroxyproline Vitamins (e.g. B12) Minerals (e.g. available P)

FO: source of beneficial fatty acids

•

Omega-3 to meet consumer expectations

FM & FO cost

Source: Tacon & Metian (2008)

Alternatives to FM & FO: what does the sea have to offer?

•

Seafood processing by-products from sustainably managed fisheries

•

New business opportunities

Shrimp processing

Recycled 30% Residues (70%) Liquid Solids

Photos: http://dailyfoodporn.wordpress.com/tag/nordic-shrimp/

Shrimp residue meal

Shrimp residue meal

70 60 50 40 30 20 10 0 Protein Lipid Ash Fiber Proximate composition P

3,0 2,0 1,0 0,0 7,0 6,0 5,0 4,0

Shrimp residue meal

Shrimp residue Herring Arg Cys His Iso Leu Lys Met Phe Thr Try Val EAA

Shrimp residue meal (SRM

)

6

Commercial SRM

4 2 0

Initial Final

CONCLUSION

1. SRM:

•Candidate alternative to $$$ sources of beneficial nutrients

2. Next steps

•Approval by regulatory agencies

LABORATORIES AND ANALYSIS SERVICES: BIOCHEMICAL CHARACTERIZATION OF MARINE PRODUCTS AND COPRODUCTS Josée Boudreau, PhD, Marie-Hélène Thibault, PhD, Claude Pelletier, M.Sc, Mathieu Ferron, M.SC

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MARINE PROCESSING COPRODUCTS

Over the years, our multidisciplinary team has developped an expertise in the screening and characterization of several marine processing coproducts such as:

Sea cucumber

• Mouth • Anus • Viscera

Arctic shrimp

• Cephalothorax • Shell • Protein waste

Lobster

• Head • Head • Tail • Viscera

Snow crab

• Hepatopancreas • Shell

Herring

• Milt • Roe 26

CHARACTERIZATION OF MARINE COPRODUCTS

In a first step,

proximate analysis

, using standard methods, reveals the major constituents in the products under study

Immature herring roe Herring milt Humidity 70% Crude protein 24% Crude fat 5% Ash 1% Humidity 77% Crude protein 18% Crude fat 3% Ash 2%

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CHARACTERIZATION OF MARINE COPRODUCTS

Each class of compounds can be probed further to gain knowledge about the product at hand and the biomolecules it contains

Immature herring roe Humidity 70% Crude protein 24%

• Marine products are known to be rich in

phospholipids

and

omega-3 fatty acids

, as well as

Crude fat 5% Ash 1%

fat-soluble biomolecules such as

vitamins

(A, D, E) and marine coproducts

pigments

• Lipids are therefore often one of the main focuses in the study of 28

MARINE COPRODUCT LIPIDS

Each

Lipid classes

• Phospholipids • Sterols • Triglycerides • Wax esters • Neutral

vs

polar lipids

Rancidity/oxidation

• Free fatty acids (FFA) • Peroxide value • p-anisidine value

Fatty acid profile

• Omega 3 • Omega 6 • EPA, DHA • Saturated • Mono-, poly unsaturated

Other bioactive molecules

• Vitamins (A, D and E) • Carotenoid pigments (Astaxanthin , β-carotene, etc.) 29

MARINE COPRODUCT PIGMENTS

Current projects in our laboratories include the detailed study and characterization of

astaxanthin

, a widespread and commercially relevant marine pigment, in shellfish processing coproducts.

Astaxanthin For animals:

• Widely used in aquaculture for flesh pigmentation improved fish growth

For humans:

• Powerful antioxidant • Anti-tumor • Anti-inflammatory • Protects against cardiovascular diseases and ulcers • Treats neurodegenerative diseases 30

ASTAXANTHIN ANALYSIS Various chemical forms

of astaxanthin naturally occur and influence its biological properties and stability. Different analytical methods are required to study these complex molecules.

Astaxanthin 1. Free or with fatty acid(s)

Free, monoester or diester

2. Geometrical isomers

All trans, 9-cis, 13-cis, etc.

3. Optical isomers

{R,R ’}, {R,S’} (MESO), {S,S’ }

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ASTAXANTHIN ANALYSIS

Current analytical capabilities in our laboratories include:   Quantification of

free

,

esterified

and

total

astaxanthin Identification of geometrical isomers (

all trans

, 9-

cis

, 13-

cis

, etc.)

High performance liquid chromatography (HPLC)

 Normal phase  Reversed phase  Chiral reversed phase  

RAMAN and FT-Infrared microspectroscopy

Microscope for

in situ

«

Same spot

imaging » technology for heterogeneous sample mapping Work is in progress to develop a rapid, simple,

in situ

method for the identification of astaxanthin optical isomers in different products 32

Fishery coproduct as nutraceutics (natural health products and functional foods): Innovation based technology

Neurodegeneration 1. Anti-diabetes 2. Anti-inflammatory 3. Anti-obesity 4. Neuroprotection Diabetes Insulin resistance Obesity Inflammation

Valorization of Coproducts from fish processing plants

 CZRI  Purification  Analyses  Process development  Pilot scale unit  NRC- Halifax  Bioguided fractionation  Purification  Adipocytes cell model (Obesity)  NRC- Charlottetown 

in vitro

and

in vivo

diabetes model  Bioguided fractionation  Dalhousie University 

in vitro

and

in vivo

Alzheimer model Jacques Gagnon

CZRI phases

Aquaculture or marine biomass • Animal feed • Natural health products (NHPs) • Human food products New, added-value products CZRI • Product formulation • Product stability • Market opportunities Product development Lead bioactives Chemical analysis • Fish cuttings • Shrimp • Sea cucumbers Bio-analysis • Natural product chemistry extraction • Chemical fractionation • Chemical characterization • •

In vitro

bioassays

In vivo

models • Scale up • Food technology centers • Partnering initiatives

Dr. Bob Chapman

Summary

• Extracts and protein hydrolysates prepared: ~100 • Focus on lead products that would be beneficial in metabolics disorders (e.g. pre-diabetes, obesity), be neuroprotective, or anti-inflammatory •Shrimp extracts and herring milt hydrolysates gave good activity for antidiabetic and neuroprotective effects •These should be further assessed by the development of health supplement ingredients with animal model of alzheimer’s disease and diabetes Jacques Gagnon

Funding Agencies

• Atlantic Canada Opportunities Agencies (ACOA)- Atlantic Innovation Fund (AIF) •New Brunswick Innovation Fund (NBIF) •Industrial Partners: ex: Island Fishermen Cooperative Association •New Brunswick Department of Agriculture, Aquaculture and Fisheries Jacques Gagnon

Patent No.

Kind WO 2014138920 A1 Date Sep 18, 2014 Application No.

WO 2014-CA251 Date Mar 13, 2014

What is in the pipeline

 Patent published … more to come with antidiabetic activities of shrimp oil  Market feasability study with shrimp oil in progress  Animal model of diabetes and neurodegeneration with shrimp oil and herring milt hydrolysate to confirm positive effects are in progress  Looking for a commercial receptor partner  Data collection in animal models to support a human clinical trial with shrimp oil (NHP)  Purification process of shrimp oil for human use at lab scale- with scaling up at industrial level Jacques Gagnon

THANK YOU

Questions?