Transcript Slide 1
Oregon Wolfe Barley Map 187 classical loci 1H 0 7 12 17 22 25 28 30 36 49 59 64 79 2H BCD1434 DsT-66 Act8A RbgMD MWG837B scind00046 ABC165C Bmac0399 GBM1007 BCD098 GBM1042 Bmag0211 BG369940 ABC160 109 JS10C 119 126 MWG706A KFP170 150 151 152 160 162 MWG2028 ABC261 KFP257B WMC1E8 MWG912 ABG387A scssr04163 166 0 1 13 41 45 47 50 62 68 69 72 75 77 90 96 102 105 110 111 112 115 133 150 152 161 173 176 179 185 193 3H ABG058 scind02622 ABG008 scssr10226 scssr07759 GBM1066 Pox scssr03381 Hot1 Ebmac0684 scssr02236 scssr00334 ABG356 GBM1023 scsnp03343 vrs1 Bmag0125 DsT-41 MWG503 GBM1062 KFP203 MWG882A ABG072 Ebmc0415 cnx1 Zeo1 GBM1019 Aglu5 Aglu4 MWG720 GBM1012 MWG949A scssr08447 4H 0 6 9 12 GBM1074 MWG798B Dst-27 BCD706 37 41 45 48 63 65 73 alm Bmac0209 ABC325 DsT-67 scssr25691 ABG377 Bmag0225 98 123 ABG499 scsnp00940 0 20 23 29 30 31 35 41 44 50 52 60 61 68 80 81 84 93 95 96 102 113 114 117 127 5H MWG634 MWG077 HVM40 CDO542 scsnp01648 CDO122 hvknox3 ABC303 scssr20569 CDO795 DST-46 scind03751 scind04312a Tef2 scind01728 GBM1020 Bmag0353 scind10455 scssr14079 ABG472 GBM1059 KFP221 Ebmac0701 MWG652B GBM1048 Hsh HVM67 KFP241.1 ABG601 6H 0 7 12 13 scssr02306 MWG618 ABC483 ABG610 41 48 61 62 63 ABG395 scssr02503 NRG045A scsnp04260 Ale 91 94 96 100 111 ABC302 scind16991 scssr15334 scsnp06144 srh 135 142 148 153 RSB001A scsnp00177 0SU-STS1 ABG003B 147 ABG004 160 165 scind02281 MWG883 183 191 HVM62 ABC805 184 188 Tef3 MWG877 202 ABC172 203 ABG496 224 228 229 236 239 253 255 E10757A scsnp02109 ABG391 JS10B ABC622 DsT-33 MWG602A scssr03906 0 7H Bmac0316 0 16 24 36 48 55 67 70 73 75 76 87 97 103 107 134 142 145 153 157 170 171 173 MWG602B JS10A GBM1021 GBM1068 BG299297 HVM31 rob scssr02093 Bmag0009 ABG474 ABG388 scsnp21226 MWG820 GBM1008 scind04312b GBM1022 Bmac0040 DsT-32B DsT-28 DsT-74 MWG798A MWG514 31 33 72 73 74 87 91 94 103 104 108 124 128 136 137 138 149 151 169 179 184 191 215 218 ABG704 GBSS-I AW982580 CDO475 MWG089 ABG380 scsnp00460 ABC255 ABC165D scssr15864 GBM1030 MWG808 DAK642 scsnp00703 MWG2031 nud lks2 ABC1024 Bmag0120 DsT-30 WG380B ABC310B Ris44 ABC253 ABG461A WG380A DsT-69 GBM1065 KFP255 ThA1 OWB – D 2-row, normal starch, hulled, long awn Vrs1Vrs1, GBSS-1GBSS-1, NudNud, LKs2Lks2 VVWWNNLL _V______ _W_________N__L_______ _V______ _W_________N__L_______ Locus abbreviations and alleles Vrs1 = V: V, v GBSS = W: W,w Nud = N: N,n LKs2 = L: L,l OWB – R 6-row, waxy starch, hull-less, short awn vrs1vrs1,gbss-1gbss-1,nudnud,lks2lks2 vvwwnnll _v______ _w_________n__l_______ _v______ _w_________n__l_______ Goal 2 row, waxy, hulled, short awn VVwwNNll OWB – D 2-row, normal starch, hulled, long awn OWB – R 6-row, waxy starch, hull-less, short awn VVWWNNLL vvwwnnll X V v w n l W N L Vv Ww Nn Ll F1 _V______ _W_________N__L_______ _v______ _w__________n__l_______ Pachynema in meiosis in F1 plant showing paired homologous chromosomes (bivalents) _V______ _W_________N__L_______ _V______ _W_________N__L_______ _v______ _w__________n__l_______ _v______ _w__________n__l_______ Pachynema in meiosis in F1 plant showing paired homologous chromosomes (bivalents). “Option 1” _V______ _W__________________ _V______ _W__________________ _v______ _w___________________ _v______ _w___________________ Gametes VW VW vw vw DH plants VVWW VVWW vvww vvww Pachynema in meiosis in F1 plant showing paired homologous chromosomes (bivalents). “Option 2” _V______ _w__________________ _V______ _w__________________ _v______ _W___________________ _v______ _W___________________ Gametes Vw Vw vW vW DH plants VVww VVww vvWW vvWW Gametes VW Vw vW vw DH VVww vvWW vvww VVWW parental NonNonparental parental parental .25 .25 .25 .25 Goal : 2 row, waxy, hulled, short awn VVwwNNll Gametes VW DH Vw VVWW VVww vW vw vvWW vvww parental NonNonparental parental parental .25 .25 .25 .25 expected 20.5 20.5 20.5 20.5 observed 20 15 25 22 Independent assortment due to random alignment of non-homologous chromosomes at Metaphase I – VVww. Chi square = 2.7, p = 0.44. (3df) parentals = non-parentals = independent assortment Pachynema in meiosis in F1 plant showing paired homologous chromosomes (bivalents) . “Option 1, again” _V______ _W_________N__L_______ _V______ _W_________N__L_______ _v______ _w__________n__l_______ _v______ _w__________n__l_______ Pachynema in meiosis in F1 plant showing paired homologous chromosomes (bivalents). “Option 1, again - with single crossover, option middle chromatids” _V______ _W_________N__L_______ _V______ _W_________N__L_______ _v______ _w__________n__l_______ _v______ _w__________n__l_______ Goal 2 row, waxy, hulled, short awn VVwwNNll X Gametes VWNL VWnl vwNL vwnl DH plants VVWWNNLL VVWWnnll vvwwNNLL vvwwnnll Pachynema in meiosis in F1 plant showing paired homologous chromosomes (bivalents). “Option 2, again – with single crossover, with middle chromatids” _V______ _w_________n__l_______ _V______ _w_________n__l_______ _v______ _W_________N__L______ _v______ _W_________ N__L______ Goal 2 row, waxy, hulled, short awn VVwwNNll X Gametes Vwnl VwNL vWnl vWNL DH plants VVwwnnll VVwwNNLL vvWWnnll vvWWNNLL Pachynema in meiosis in F1 plant showing paired homolowous chromosomes (bivalents). “Option 2, again – single crossover with outside chromatids” _V______ _w_________n__l_______ _V______ _w_________n__l_______ _v______ _W_________N__L______ _v______ _W_________ N__L______ Goal 2 row, waxy, hulled, short awn VVwwNNll X Gametes VwNL Vwnl vWnl vWNL DH plants VVwwNNLL VVwwnnll vvWWnnll vvWWNNLL Pachynema in meiosis in F1 plant showing paired homologous chromosomes (bivalents). “Option 2, again – single crossover with 1 middle and 1 outside chromatid” _V______ _w_________n__l_______ _V______ _w_________n__l_______ _v______ _W_________N__L______ _v______ _W_________ N__L______ Goal 2 row, waxy, hulled, short awn VVwwNNll X Gametes Vwnl VwNL vWNL vWnl DH plants VVwwnnll VVwwNNLL vvWWNNLL vvWWnnll Goal 2 row, waxy, hulled, short awn VVwwNNll Gametes WN DH Wn WWNN WWnn wN w wwNN wwnn parental NonNonparental parental parental .25 .25 .25 .25 expected 20.5 20.5 20.5 20.5 observed 27 18 13 24 Independent assortment due to crossovers between loci on same chromosome – wwNN. Chi square = 5.59, p = 0.13. (df) parentals = non-parentals = independent assortment Pachynema in meiosis in F1 plant showing paired homologous chromosomes (bivalents). “Option 1, again – with single crossover, middle chromatids” _V______ _W_________N__L_______ _V______ _W_________N__L_______ _v______ _w__________n__l_______ _v______ _w__________n__l_______ Goal 2 row, waxy, hulled, short awn VVwwNNll X Gametes VWNL VWNl vwnL vwnl DH plants VVWWNNLL VVWWNNll vvwwnnLL vvwwnnll Gametes NL Nl nL nl DH NNll nnLL nnll NNLL parental NonNonparental parental parental 45 2 10 36 expected 20.5 20.5 20.5 20.5 observed 37 3 9 33 Parentals > non-parentals = linkage Linkage is calculated as the total non-parental types/ total population size 12/82 = 14.6% recombination = 15.0 Kosambi cM; 17.3 Haldane cM. 124 cM – 108 cM = 16 cM per OWB classical map Distance (cM) Haldane =-0.5*LN(1-2*.r) Distance (cM) Kosambi=0.25*LN((1+2*r)/(1-2*r)) Mapping Functions Oregon Wolfe Barley Map 187 classical loci 1H 0 7 12 17 22 25 28 30 36 49 59 64 79 2H BCD1434 DsT-66 Act8A RbgMD MWG837B scind00046 ABC165C Bmac0399 GBM1007 BCD098 GBM1042 Bmag0211 BG369940 ABC160 109 JS10C 119 126 MWG706A KFP170 150 151 152 160 162 166 MWG2028 ABC261 KFP257B WMC1E8 MWG912 ABG387A scssr04163 0 1 13 41 45 47 50 62 68 69 72 75 77 90 96 102 105 110 111 112 115 133 150 152 161 173 176 179 185 193 3H ABG058 scind02622 ABG008 scssr10226 scssr07759 GBM1066 Pox scssr03381 Hot1 Ebmac0684 scssr02236 scssr00334 ABG356 GBM1023 scsnp03343 vrs1 Bmag0125 DsT-41 MWG503 GBM1062 KFP203 MWG882A ABG072 Ebmc0415 cnx1 Zeo1 GBM1019 Aglu5 Aglu4 MWG720 GBM1012 MWG949A scssr08447 4H 0 6 9 12 GBM1074 MWG798B Dst-27 BCD706 37 41 45 48 63 65 73 alm Bmac0209 ABC325 DsT-67 scssr25691 ABG377 Bmag0225 98 123 ABG499 scsnp00940 0 20 23 29 30 31 35 41 44 50 52 60 61 68 80 81 84 93 95 96 102 113 114 117 127 5H MWG634 MWG077 HVM40 CDO542 scsnp01648 CDO122 hvknox3 ABC303 scssr20569 CDO795 DST-46 scind03751 scind04312a Tef2 scind01728 GBM1020 Bmag0353 scind10455 scssr14079 ABG472 GBM1059 KFP221 Ebmac0701 MWG652B GBM1048 Hsh HVM67 KFP241.1 ABG601 6H 0 7 12 13 scssr02306 MWG618 ABC483 ABG610 41 48 61 62 63 ABG395 scssr02503 NRG045A scsnp04260 Ale 91 94 96 100 111 ABC302 scind16991 scssr15334 scsnp06144 srh 135 142 148 153 RSB001A scsnp00177 0SU-STS1 ABG003B 147 ABG004 160 165 scind02281 MWG883 183 191 HVM62 ABC805 184 188 Tef3 MWG877 202 ABC172 203 ABG496 224 228 229 236 239 253 255 E10757A scsnp02109 ABG391 JS10B ABC622 DsT-33 MWG602A scssr03906 0 7H Bmac0316 0 16 24 36 48 55 67 70 73 75 76 87 97 103 107 134 142 145 153 157 170 171 173 MWG602B JS10A GBM1021 GBM1068 BG299297 HVM31 rob scssr02093 Bmag0009 ABG474 ABG388 scsnp21226 MWG820 GBM1008 scind04312b GBM1022 Bmac0040 DsT-32B DsT-28 DsT-74 MWG798A MWG514 31 33 72 73 74 87 91 94 103 104 108 124 128 136 137 138 149 151 169 179 184 191 215 218 ABG704 GBSS-I AW982580 CDO475 MWG089 ABG380 scsnp00460 ABC255 ABC165D scssr15864 GBM1030 MWG808 DAK642 scsnp00703 MWG2031 nud lks2 ABC1024 Bmag0120 DsT-30 WG380B ABC310B Ris44 ABC253 ABG461A WG380A DsT-69 GBM1065 KFP255 ThA1 Pachynema in meiosis in F1 plant showing paired homologous chromosomes (bivalents) _V______ _w_________n__ l_______ _V______ _w_________n__ l_______ _v______ X X _W_________N__L______ _v______ _W_________ N__L______ Goal 2 row, waxy, hulled, short awn VVwwNNll Gametes Vwnl VwNl vWnL vWNL DH plants VVwwnnll VVwwNNll vvWWnnLL vvWWNNLL