Transcript Comparative analysis of aberration yields induced by

Comparative analysis of aberration yields
induced by radiation with different LET
(Linear Energy Transfer)
University of Warsaw

University of Gdańsk


Magdalena Rojek
Monika Szymanska
AGH University of Science
and Technology



Anna Kaminska
Dominika Kedzierska
Ewa Lagudza
Jakub Pietrzak UW
THE DREAM TEAM
2
Laboratory of Radiation Biology
Marta
Deperas- Kaminska (supervisor)
Ekaterina Zaytseva
3
The practice purposes:

Theoretical introduction to cytogenetic methods
used in biodosimetry

Instructions of safety rules of work with human
blood samples.

To learn how to irradiate human lymphocytes
and culture them under experimental
conditions.

Be able to carry out a simple experiment and
analysis with irradiated cells
4
SCHEME OF THE EXPERIMENT
Incubation for the standard metaphase analysis
Radiation
colcemid
47h
Fixation
50h
Standard
light
microscope
5
Our experiment
1.
2.
Irradiation of blood samples with different LET
radiation
Lymphocytes culture (48 hours)
Picture 2 Lymphocyte
Picture 1 Blood
http://en.wikipedia.org/wiki/Image:SEM_Lymphocyte.jpg
http://en.wikipedia.org/wiki/Lymphocyte
6
3.
4.
5.
Lymphocytes fixation
Giemza staining
Analysis of chromosomes aberrations
Picture 3 Chromosomes
Picture 4 Chromosomes 2
http://humogef.ifj.edu.pl/zaklad/foto13.jpg
http://humogef.ifj.edu.pl/zaklad/foto13.jpg
7
Unstable aberrations - dicentrics, acentric
fragments and centric and acentric rings in Giemsa
stained metaphase
Centric ring
ace
deletion
Acentric ring
Dic
8
RESULTS:
The increase of aberration yields with increase of radiation LET in
human peripheral blood lymphocytes is observed.
400
aberration frequency
sum of all kinds of aberration per 100 cells
number of dicentrics per 100 cells
300
200
100
0
0
0.3
gamma
0.5
12
protons
12
C
LET (keV/um)
20
7
Li
55
11
B
9
Acknowledgements
Special thanks to:

Dmitri Vladimirovich Fursaev

Wladyslaw Chmielowski, Roman Zawodny

Marta Deperas-Kaminska, Katia Zaytseva
10
THANKS FOR YOUR ATTENTION
11