Transcript Preparing for a lab - University of Arizona

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Welcome to
Bio181L!
Contemplating the nuts & bolts of Life
“It’s atoms all the way down”
About me...
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Graduated U of A with bachelors of science in EEB
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Love animals, traveling, volleyball and snowboarding
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Preparing for graduate school in
oceanography/marine biology
Now its your turn!
• Major
• Hobbies
• Plans for the future
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What 181L is
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Thinking, Understanding, Investigating, Evaluating
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Not arrive, assembly line, leave
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Building basics and applying them in many different ways
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Manual pp. ix & xi should help
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Not sync’ed with most lecture sections, but internally coherent &
mutually reinforcing
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Office Hours
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I will hold one office hour per week
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Thursdays 12:30am – 1:30pm (will extend if necessary)
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Also by appointment if necessary
Email me in advance if you are coming or I won’t show up
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181 Lab ACCOUNTS
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http://blc.arizona.edu/courses/181lab (Safari home page)
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Click ‘Create Accounts’
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Your section number is:
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Do not use UAnetID password for this account!!
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Bio181L_Go is the way to do your work. Browser bad
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80% of success is just showing up*
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Absences must be excused by Asya Roberts in BSE109 prior to
making up. Contact her as soon as possible. Really.
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Late is absent. Leaving early is absent.
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Labs cannot be offered week after they are delivered; Avoid
missing a lab
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Missing 2 labs => you’ll be dropped from the course--excused
or otherwise (b/c you’ll have little shot at learning what you
need)
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Make up labs – written documentation from Asya showing
excused absence (same week that lab was delivered)
*--Woody Allen
Policies & Grading
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Syllabus (Linked on course homepage)
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Honor code & Plagiarism (Manual, p. xiii)
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Sign plagiarism contract
Assignments
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On-line assessments/tutorials (10%)
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In-class quizzes (15%)
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Lab assignments (LABAs--Lab Activity Based Assignments) (50)
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Lab Projects & reports (combine to 25%)
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Every assignment is posted & reported on basis of 100%
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For electronic assignments, log in again to see your recorded score
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Also reported to you at the end
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Doing Homework
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Use Bio181L_Go Program! Download from software link
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All assignments should be in this program unless otherwise
stated
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View tutorial movies on how these online assignments work
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ALL homework due by 10pm the night before your lab section
meets
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Use calendar as a reference for what homework is due when
(Demo)
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LOG IN for credit
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Note – quiz  answer key Friday afternoon
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My Webpage
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Get there via homepage* => Instructors => Sxn#
*http://blc.arizona.edu/courses/181Lab
(Lab Man yellow page)
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Web page operation
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Quizzes
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Every lab, first 15 min
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Will highlight topics from the previous lab and one question about
the lab being performed that day (read manual!)
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No quiz make-ups for being late
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There will be a quiz at the end of lab today
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Clean up!!! Points off quiz if not satisfactory
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Bottom right of homepage
Written Assignments
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Most written assignments assigned for homework will be turned
in on D2L via the dropbox (Demo)
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I will leave feedback
Don’t hesitate to ask me why something was graded the way it
was
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Lab Manual
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Read it!
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Vocab at the end of every lab
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Helpful topics in appendices
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Classroom Policies
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No cell phones, in emergency go outside please
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No food/drink in the lab (water ok but away from computers)
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DRC Students
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See me after class if you have questions or require action on my
part
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How?
Why?
the Tao of Molecules
How molecules feel & the world they live in
Primary goals
• Create understanding by observation,
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reasoning
Chemical foundations for the course:
• Water & its properties
• non-watery things
• Know molecules as real & tangible things
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When a journalist asked the great physicist Richard
Feynman what single sentence would best
encapsulate all science so far if it were to be the
sole surviving scrap of all we knew, he replied,
“The world is made of atoms.”
From The Secret of Scent, Luca Turin p.28
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Atoms: They’re how life works
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DNA, RNA: C, H, N, O, P, [Mg++]
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Carbs: C, H, O
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Protein: C, H, N, O, (S), (P), [traces]
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Membranes: C, H, N, O, P, (S)
What cannot be done with assemblages of these atoms*
cannot be done by living organisms, nor their cells, nor their
spit, etc.
*OK, fine, there’s the occasional role for Ca++, etc.
Who am I?
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At birth, # protons = # electrons
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Atoms seek completion, which means outermost electron set = 8
(hydrogen, helium it’s just 2)
Freeman Fig. 2.1a
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Symbolizing
Atoms & Molecules
Coloring your world
Grey
White
Blue
Red
Yellow
Gold
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Views of Water
(There will be a test)
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H2O
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H
See lab manual, p. 0-3
O
H
Interacting with H2O
To your StructViewers!
Desktop => Bio181L_Go
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Doing it
Computers, stations
Write your group name down; otherwise you
can’t retrieve your work!
Desktop Tao Stack 181L (not honors)
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Pipetman Demo
Group names
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1 – dolphin3
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2 – elephant3
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3 – penguin3
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4 – turtle3
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5 – sloth3
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6 – panda3
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7 – cheetah3
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How to draw molecules
Carbon Hydrogen Oxygen Nitrogen
Figure is from Lab Manual p. 0-5
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TA-lead discussions
Alcohols – similarities/differences
Propanol + Water + Salt
“Salting out”
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It’s a real term; used to refer to a situation where the addition of
salt (portable charges) alters the solubility of other molecules in
water
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Why should this be?
Evaporation
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First things first--what is it? What’s going on in terms of molecules
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Experiment: Ethanol and water on your arm: which is cooler & why?
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Prediction: based on molecular weight, which should evaporate
more quickly--H2O (2 x 1 + 1 x 16) or CH3CH2OH (5 x 1; 2 x 12; 1 x 16)
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Which do you expect to evaporate first? Why?
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Salt--ever tasted your sweat? Or anybody else’s for that matter?
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What benefit might there be to adding NaCl to water that you
are intending to evaporate?
Clean up!
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Oil waste in the hood
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Ethanol waste in the sink
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Anything too messy wadded up and discarded
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There is a bucket of soap water by the sink, all empty
(cleaned) glassware here
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Check out goopy stuff in front of room when
finished
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What’s vocabulary homework?
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EITHER both versions of the crossword exercise
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OR VocabuWary with a better than threshold score
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For “Atoms & Molecules”, F12, that’s 60,000
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Errors count off. Slowness counts off.
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Homework
due 10 p.m. before lab
Assessor: 181 Intro ’12
Vocab Atoms/Mols: xWord(x2) or ‘Wary
Assessor: Molecular World Tutorial
Next week’s quiz will include
Concepts from today
Atom colors
Deducing partial charges (from tutorial)
Manual Ch. 2
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