Basics on Tetradyn’s BioProt using the Dow/Aegis Quat-Ammonia Chem Base

This is NOT a formal presentation document, and it needs to be read along with some others with experimental data results!

Microbial Contamination Sources:

Infiltration

TETRAD Innovation, Inc.

Filters Ducts Occupied Space People

What Are Acceptable Levels of Microbial Exposure?

For Chemicals - Threshold Limit Values, Other Mandated Limits For Microbes - Least Possible Exposure

TETRAD Innovation Inc.

Traditional Solutions

1) Housekeeping (Detergents,Bleach,Fragrances,etc.) SHORT TERM SOLUTIONS * Mask odours, problems * Adaptation by microbes TETRAD Innovation Inc.

Traditional Solutions

2) Engineering (Selection, Operation, Maintenance, Modification of HVAC Systems) DILUTION * Does not address pollution sources, may be part of the problem!

* Cost / Benefit Ratio TETRAD Innovation Inc.

Traditional Solutions

3) Industrial Hygiene (Assessment, Testing, Analysis) VAGUE CONCLUSIONS * Untrained in Microbiology * Little Guidance TETRAD Innovation Inc.

MICROORGANISMS IN BUILDINGS Strategies For Control • • • Control Nutrients - but all surfaces can be colonised!

Control Moisture - often not effective or possible.

Control Sources - prevent growth.

The AEGIS Microbe Shield permanently modifies building surfaces, even those that will get wet, so they cannot act as a source of microbial amplification

TETRAD Innovation Inc.

BUILDING BIOSPHERE

Microbial Amplification Changes: Humidity,Chemicals, Surfaces, & Nutrients

TETRAD Innovation, Inc.

Genetic Adaptation

Differences in Antimicrobials

These two types of antimicrobials differ in their: • • • • • • • • • Chemical Nature Durability Safety Mode of Operation/Action Regulatory Compliance Effectiveness Cost Verification/Quality Assurance Ease of Application TETRAD Innovation, Inc.

Types of Antimicrobials

There are 2 different types of antimicrobials available on the market: • • Conventional (leaching or migrating) Unconventional (non-leaching, bonded) TETRAD Innovation, Inc.

Conventional Leaching Antimicrobials

• Diffuse from the product to come in contact with the microbe • Leach or migrate off the product • Are consumed by microorganisms • Chemically interrupt (poison) the cell • Creates environment that allows microbial adaptation • No means of attachment TETRAD Innovation, Inc.

Conventional Antimicrobials: Examples of Leaching Technologies

• • • • • • • • Bis chlorinated phenols Organo tins (i.e. TBT) Organo metallics (Pb, As, Hg) Chitin Silver-Copper/ Zeolite Water soluble Quats Biguanide Isothiozolione TETRAD Innovation, Inc.

What is the Microbe Shield?

•A revolutionary permanent antimicrobial technology that does not rub off or migrate onto the skin •An extensively tested technology developed at Dow Corning Corporation •An antimicrobial technology safely and effectively used for over 20 years •A technology easily applied to indoor surfaces such as walls, floors, seats, air filtration systems, washrooms etc.

TETRAD Innovation Inc.

The Microbe Shield

®

Is bound to the product controlling microbes on contact

• • • • • Bonded to the surface Not consumed by microbes Mechanically stabs the cell Extended functional life Will not cause microbial adaptation TETRAD Innovation Inc.

TETRAD Innovation Inc.

How it works…..

Microbe Shield

TETRAD Innovation Inc.

TETRAD Innovation, Inc.

The Microbe Shield

TETRAD Innovation Inc.

The Microbe Shield

The cell membrane is attracted to the treated surface and then punctured by the long molecular chain

TETRAD Innovation, Inc.

The Microbe Shield

As it is drawn closer because of the positive negative ion exchange, it penetrates further into the cell membrane and….

TETRAD Innovation Inc.

The Microbe Shield

… the cell is physically ruptured by a sword-like action and then electrocuted by a positively charged nitrogen molecule thus destroying it.

TETRAD Innovation, Inc.

Government Regulations

Internationally registered in China, Europe and The Americas

TETRAD Innovation Inc.

• • • Proper registration for specific end use Reaction to misapplication Reaction to errant claims

TETRAD Technologies Group, Inc. TETRAD Innovation Inc.

TETRAD Innovation Inc.

TETRAD Innovation Inc.

TETRAD Innovation Inc.

Escherichia coli

Illustration of cell rupture from treatment with the ÆGIS Microbe Shield.

Untreated Fabric

TETRAD Innovation Inc.

Fabric treated with the ÆGIS Microbe Shield

Staphylococcus aureus

Illustration of cell rupture from treatment with the ÆGIS Microbe Shield.

Untreated Fabric

TETRAD Innovation Inc.

Fabric treated with the ÆGIS Microbe Shield

OBJECTIVE

Provide Healthy Indoor Environment •Sustain occupant loyalty •Increase occupancy •Reduce complaints

HOW

The Aegis Microbe Shield •Controls Odour-Causing Yeast & Fungi •Prevents Growth of Infectious Bacteria •Is A Safe, Effective & Durable Treatment TETRAD Innovation Inc.

Easily Applied

Batch

TETRAD Innovation Inc.

Pad Spray

Easily Verified

Bromophenol Blue (BPB) Direct Stain

•

Quick and easy verification on the product whether at the mill, the distribution center or on the retail shelf

TETRAD Innovation Inc.

Bromophenol Blue (BPB) Direct Stain

Untreated Treated

5 Minutes!

TETRAD Innovation Inc.

The Microbe Shield Difference

• Maintains long-lasting freshness • Eliminates odor created by yeast, fungus and bacteria in the product • Controls or eliminates microbial staining of the treated article • Quality, safety, durability and effectiveness all add up to a valuable feature in any product TETRAD Innovation, Inc.

The Microbe Shield Difference

• Effective after more than 40 launderings • Produced by a Fortune 500 company in an ISO-9002 facility • Unmatched safety profile • The confidence of over 30 years of effective, safe use on hosiery, active wear, medical goods, home furnishings, and numerous other consumer products TETRAD Innovation Inc.

The Microbe Shield Difference

Used on recognizable products like Odor-Eaters ® and Dr. Scholl’s ® socks, Keen Footwear and Exofficio ® sporting goods Used in performance fabrics such as surgical drapes and clean room garments Used to protect the indoor surfaces of buildings from contaminating microbes Global distribution and unparalleled marketing, technical, and customer service support TETRAD Innovation, Inc.

Hypoallergenic Rooms

• • • • Controls leading cause of asthma and allergies Improves indoor environment Reduces odours and improves freshness Long lasting and cost effective TETRAD Innovation Inc.

Ventilation Systems

• Approved by PMRA for use as a long term material preservative on duct work • Approved for use on sound liners, coils, louvers and other components • Controls microbes on chronically wet surfaces near intakes and cooling coils • Controls major contributor to SBS TETRAD Innovation, Inc.

Schools and Other Public Buildings

• • • • • Provides a “window of opportunity” to facilitate needed repairs Immediate control of microbes Addresses public concerns with chemical usage Safe and cost effective, 3 year warranty Virtually all surfaces are treatable TETRAD Innovation Inc.

Health Care Facilities

• • • Controls infectious microbes Does not promote genetic adaptation Safe for use in ICU, OR, and other sensitive areas • • Controls microbes during renovations Controls MRSA

Aspergillus

,

E. coli

,

Salmonella

, other invasive pathogens TETRAD Innovation Inc.

Features & Benefits

•Broad spectrum control - Fungi Mold, Mildew, Gram(+) and Gram(-) Bacteria, Yeasts, Algae •Improves “cleanability” on many surfaces •Controls or eliminates microbial staining and deterioration •Odorless and colorless •Cost effective application •Durable for at least 3+ years on most surfaces and up to 10 years •State of the art science and technical support •Unmatched safety profile •Won`t leach into the environment or transfer to other articles or to the skin no “zone of inhibition” in lab tests •May be applied to all non-food contact surfaces •No arsenic, heavy metals, or polychlorinated phenols TETRAD Innovation Inc.

•The confidence of more than 20 years of effective use on hosiery active wear, medical goods, undergarments, home furnishings, carpets, air filters, bedding, disposable wipes, etc

EMOTIVE BENEFITS

provided by the AEGIS Microbe Shield • • Maintains long lasting freshness • • Eliminates the smells created by mould, mildew and bacteria in the building • Controls or eliminates microbial staining of the treated surface Reduces the risk of surface contamination Controls a major contributor to SBS TETRAD Innovation, Inc.

TECHNICAL BENEFITS

provided by the AEGIS Microbe Shield • • • • Effective for three years or more Produced by a Fortune 500 company in an ISO 9002 facility Unmatched safety profile - applied only by trained applicators The confidence of more than 20 years of effective, safe use on hosiery, active wear, medical goods, home furnishings, and numerous other consumer products as well as in buildings TETRAD Innovation, Inc.

The Microbe Shield is available through TETRAD Technologies Group, Inc

Contacts: Dr.Martin Dudziak (CSO) 757-847-5511 in Canada and the USA TETRAD Innovation, Inc.