Transcript BioFire (FilmArray) Multiplex PCR Assays

BioFire (FilmArray)
Multiplex PCR Assays
Teresa Finken, MT(ASCP)
Panels Available
Respiratory Viral Panel
Blood Culture Identification Panel
Gastrointestinal Panel
Methodology
Multiplex Real-Time PCR Assay
These panels test for multiple targets
simultaneously
This methodology is real-time PCR
meaning that it amplifies and detects at
the same time
What does this Mean to You?
One sample
One test
Multiple results
1.5-hr turnaround time
Respiratory Viral Panel Targets
 Viruses
 Adenovirus
 Coronavirus HKU1
 Influenza B
 Parainfluenza 1
 Coronavirus NL63
 Parainfluenza 2
 Coronavirus 229E
 Parainfluenza 3
 Coronavirus OC43
 Parainfluenza 4
 Human
Metapneumovirus
 Respiratory Syncytial Virus
 Human
Rhinovirus/Enterovirus
 Bacteria
 Influenza A
 Bordetella pertussis
 Influenza A/H1
 Chlamydophila
pneumoniae
 Influenza A/H1-2009

Influenza
A/H3tapneumovirus
 Mycoplasma pneumoniae
Blood Culture Id Panel Targets
Gram + Bacteria

Enterococcus

Listeria monocytogenes

Staphylococcus

Staphylococcus aureus

Streptococcus

Streptococcus agalactiae

Streptococcus pyogenes

Streptococcus pneumoniae
Yeast
Gram – Bacteria

Acinetobacter baumannii

Haemophilus influenzae

Neisseria meningitidis

Pseudomonas aeruginosa

Enterobacteriaceae

Enterobacter cloacae complex

Escherichia coli

Klebsiella oxytoca

Klebsiella pneumoniae

Candida albicans

Proteus

Candida glabrata

Serratia marcescens

Candida krusei

Candida parapsilosis

Candida tropicalis
Antibiotic Resistance

mecA - methicillin resistant

vanA/B - vancomycin resistant

KPC - carbapenem resistant
Gastrointestinal Panel Targets
Bacteria

Campylobacter (jejuni, coli and
upsaliensis)

Clostridium difficile (toxin A/B)

Plesiomonas shigelloides

Salmonella

Yersinia enterocolitica

Vibrio (parahaemolyticus, vulnificus
and cholerae)

Vibrio cholerae

Diarrheagenic E. coli/Shigella

Enteroaggregative E. coli (EAEC)

Enteropathogenic E. coli (EPEC)

Enterotoxigenic E. coli (ETEC) lt/st

Shiga-like toxin-producing E. coli
(STEC) stx1/stx2

E. coli O157

Shigella/Enteroinvasive E. coli
(EIEC)
Viruses

Adenovirus F 40/41

Astrovirus

Norovirus GI/GII

Rotavirus A

Sapovirus (I, II, IV and V)
Parasites

Cryptosporidium

Cyclospora cayetanensis

Entamoeba histolytica

Giardia lamblia
Sample Requirements
Respiratory Viral Panel
Blood Culture ID Panel
o Nasal Wash
o Blood culture bottle
that has been flagged
positive by the Bacti
Alert or Versatrek
Analyzers
o Nasal Swab
o Sputum
o Respiratory specimen
in Viral Transport
Medium
Gastrointestinal Panel
o Raw stool to be
transferred to ETM
o Stool in ETM
Positive Results Since
Implementaion
These are just a few…
 Limited exposure to potentially hazardous bacterial
isolates in blood cultures
 Faster treatment and more guided treatment of patients
at the point of entry and after admission
 Quick diagnosis
 Faster antimicrobial therapy implementation to speed
recovery and reduce morbidity
 Admitting a patient versus not admitting a patient
Questions…