Transcript Natural Products Chemistry

Natural Products Chemistry
Laboratory
Fall Semester 2014
Sunday from 8-12am , organic chemistry laboratories
Chemistry Department, Faculty of Science
Instructors
• Dr. Amir E. Wahba, Lab supervisor
• Research Interest:
• Marine natural products, total synthesis of natural products, cancer chemotherapy,
pseudo LDL nanoparticles for targeted delivery of cancer chemotherapy, new synthetic
methodologies, medicinal chemistry, isolation and structure elucidation of bioactive
natural products.
• Contact:
• Office hours: Saturday, by appointments
• Email: [email protected]
• Ahmed Sheta, Teaching Assistant (TA)
• Hani El-Beheiry , Teaching Assistant (TA)
What are Natural Products?
What are Natural Products?
What are Natural Products?
• Living organisms produces natural products for several reasons:
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Live
Grow
Reproduce
Defense
Communicate
• Examples of living organisms that produces natural products:
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Bacteria
Fungi
Plants
Marine organisms (sponges, alga...etc)
How do plants produces organic compounds?
Biosynthesis
Metabolism
• Primary Metabolism: All the chemical reactions necessary for the live
of the organism by itself.
• Primary Metabolites: All the chemical products involved in the
Primary Metabolism.
• Secondary Metabolites: All the chemical products that are produced
by the living organism but are NOT involved in the primary
metabolism. Not necessary for the live of the organism by itself but
useful for interaction with others (chemical communication, chemical
defenses).
What is Natural Products Chemistry?
• Two major fields associated with natural products chemistry:
• Isolation, purification and structure elucidation of natural products:
• Different isolation and purification techniques
• Chemical and spectral methods for structure elucidation
• Total synthesis of natural products:
• Knowledge about different synthetic methodologies
• Strong knowledge of stereoselective reactions
• Very challenging and difficult field
Course outlines
• Course Description:
• This course is designed to give students an overview on the different isolation and purification
techniques
• Learning outcomes:
• Students should learn the basics of the different chromatographic techniques as well as writing a wellorganized lab note. Knowledge about natural products are expected with the conjunction with the
course number.
• Textbox and material: All experiments will be available online
• Course requirements and grading:
• There will be a weekly quiz and lab report that need to be completed and delivered before the
beginning of the lab. Additional assignments are also expected specially when there are an extra time
left in the lab.
• The following grading system will be applied:
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Attendance 10%
Lab report 15%
Quiz 10%
Behavior and responsibility 15%
Note Book 10%
Regulations
• All students are expected to follow the safety and waste disposal rules. Lab
goggles, coats and gloves are mandatory for all students and TA’s. No student
will be allowed in the lab without those items.
• Labs start 8:00 am sharp! Please make all your arrangements to be here in time.
• Quizzes and lab assignments will be taken at the seminar room in located at the
chemistry Department.
• A positive attitude and willingness to learn is essential to be successful.
• You must come to lab prepared and on time. Read the experiments ahead of
time.
• Write down questions that come up as you read the experiment. Ask questions.
• Although good results are the ultimate goal, determining why an experiment did
not work and understanding the experiment is equally or more valuable.
How to write your lab note?
Otto Hahn
Nobel Prize in Chemistry, 1944
Radioactivity
Lab Reports
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Lab reports will contain the following information:
• Introduction:
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Provide a short informative paragraph about the chemical being evaluated and how it is used.
Include information that describes the practical application of the compound or procedure in question and a statement of the objective.
There may be more than one objective for each experiment.
• Data ( include calculations):
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Graphs, tables, spectra and chromatograms are part of the data and must also be included in the report .
• Result and Discussion:
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Describe the procedure briefly and the results of your experiment. Example: If you separated compounds, tell how it was done. If you
synthesized a compound, tell how it was made and in what yield. Support your statements with your data. Data report in the data sections
should be referred in the result and discussion. Explain the data/results and what they imply. Discuss unusual observations, errors and
anything that will better your understanding of the principles involved. Make suggestions that would solve the stated problems. Make this
section brief and concise.
• Conclusion:
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A brief statement to answer the problem posed in the objective.
• Coffee break
Experiments outline
Date
Experiment
October 12
Introduction and orientation
October 19
Isolation of caffeine from tea
Liquid-liquid extraction
October 26
Isolation of piperine from black
pepper
Solid-liquid extraction
November 2
Isolation of limonene from
Distillation
oranges
Isolation of cholesterol from egg Thin layer chromatography
lipids
November 9
November 16
November 23
Isolation of carotenoids from
spinach
Dyes lab
November 30
Dyes lab
Techniques
Column chromatography