Transcript Neurologia
Óragének (és a melatonin) Rékási Zoltán PTE ÁOK Anatómiai Intézet 2 3 birds mammals 4 5 6 7 MEDIUM SAMPLE 5 % CO2 + 95 % AIR AIR WITHDRAWAL SAMPLE 37 Co WATER BATH WATER JACKET . V. Cs CELLS + SEPHADEX FOR SAMPLE DILUTION PERISTALTIC PUMP FRACTION COLLECTOR 8 Melatonin elválasztás explantált patkány tobozmirigyből normál in vitro megvilágítási ciklusban 9 Melatonin elválasztás explantált csirke tobozmirigyből normál in vitro megvilágítási ciklusban 50 40 30 20 10 0 18 0 6 12 18 0 6 12 18 0 6 12 18 0 6 hours 10 LIGHT NORADRENERGIC and PEPTIDERGIC PATHWAYS Adrenergic and VPAC-RECEPTORS SIGNAL TRANSDUCTION HO CH 2 CH 2 NH2 N H SEROTONIN N-acetyltransferase (AA-NAT) AcCoA HO CH 2 CH 2 NH C CH3 O N H N-ACETYLSEROTONIN hydroxyindol-o-methyltransferase (HIOMT) SAdM CH3O MELATONIN SYNTHESIS MELATONIN RELEASE CH 2 CH 2 NH C CH3 O N H MELATONIN 11 LIGHT PHOTORECEPTORS SIGNAL TRANSDUCTION ? ? CLOCK (PACEMAKER) Clock-genes ? HO CH 2 CH 2 NH2 N H SEROTONIN N-acetyltransferase (AA-NAT) AcCoA HO CH 2 CH 2 NH C CH3 O N H N-ACETYLSEROTONIN hydroxyindol-o-methyltransferase (HIOMT) SAdM CH3O MELATONIN SYNTHESIS MELATONIN RELEASE CH 2 CH 2 NH C CH3 O N H MELATONIN 12 13 gene expression CLOCK BMAL1 Clock Bmal1 PER TIM period timeless Activation Inhibition Four-gene core transcription-translation loop DELAY time 14 15 Clock-genes in Drosophila and mammals PERIOD1 PERIOD2 PERIOD PERIOD3 NLS NLS bHLH/PAS NLS NLS TIMELESS CLOCK CYCLE Doubletime Cryptochrome BMAL1 casein kinase I epsilon Cryptochrome1 16 Cryptochrome2 17 18 Expression pattern of cAANAT, cBmal1 and cClock mRNA in chicken pineal gland 19 Expression pattern of cAANAT, cBmal1 and cClock mRNA in chicken retina 20 Expression of retinal and pineal cAANAT mRNA in chicken housed in constant darkness for 1 day or 1 week retina pineal 21 Expression of retinal and pineal cBmal1 mRNA in chicken housed in constant darkness for 1 day or 1 week retina pineal 22 Expression of retinal and pineal cAANAT mRNA in chicken housed in constant light for 1 day or 1 week retina pineal 23 Expression of retinal and pineal cBmal1 mRNA in chicken housed in constant light for 1 day or 1 week retina pineal 24 Model of circadian clock-work within an individual retinal or pineal photoreceptor cell Clock Resetting the clock by light Bmal1 cCry2 cCry1 cPer2 cPer3 cAANAT Output/melatonin 25 26