Transcript Jeopardy

Chapter 3: Observing Microorganisms Through a Microscope
Microscopy
Electron
Microscopy
Preparing
Smears for
Staining
Staining
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Compound
Microscope
FINAL ROUND
© 2013 Pearson Education, Inc.
Topic 1: Compound Microscope
$100 Question
What is the function of the diaphragm on a
compound microscope?
a.
b.
c.
d.
magnifies the specimen
focuses light through the specimen
holds the microscope slide in position
controls the amount of light entering the
condenser
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 1: Compound Microscope
$100 Answer
What is the function of the diaphragm on a
compound microscope?
a.
b.
c.
d.
magnifies the specimen
focuses light through the specimen
holds the microscope slide in position
controls the amount of light entering the
condenser
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 1: Compound Microscope
$200 Question
The ocular lens is used to
a.
b.
c.
d.
magnify the image from the objective lens.
improve resolution.
increase the light.
decrease the refractive index.
ANSWER
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© 2013 Pearson Education, Inc.
Topic 1: Compound Microscope
$200 Answer
The ocular lens is used to
a.
b.
c.
d.
magnify the image from the objective lens.
improve resolution.
increase the light.
decrease the refractive index.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 1: Compound Microscope
$300 Question
Brightfield illumination is produced by the
a.
b.
c.
d.
condenser.
objective.
lens.
annular diaphragm.
ANSWER
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© 2013 Pearson Education, Inc.
Topic 1: Compound Microscope
$300 Answer
Brightfield illumination is produced by the
a.
b.
c.
d.
condenser.
objective.
lens.
annular diaphragm.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 1: Compound Microscope
$400 Question
Most ocular lenses magnify specimens by a
factor of
a.
b.
c.
d.
100.
10.
1000.
2000.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 1: Compound Microscope
$400 Answer
Most ocular lenses magnify specimens by a
factor of
a.
b.
c.
d.
100.
10.
1000.
2000.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 1: Compound Microscope
$500 Question
The ability of the lenses to distinguish fine
detail and structure is called
a.
b.
c.
d.
illumination.
magnification.
refractive index.
resolution.
ANSWER
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© 2013 Pearson Education, Inc.
Topic 1: Compound Microscope
$500 Answer
The ability of the lenses to distinguish fine
detail and structure is called
a.
b.
c.
d.
illumination.
magnification.
refractive index.
resolution.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 2: Microscopy
$100 Question
Which microscope would you choose to view
the cellular contents of a microbe in its natural
state?
a.
b.
c.
d.
compound light microscope
phase-contrast microscope
darkfield microscope
electron microscope
ANSWER
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© 2013 Pearson Education, Inc.
Topic 2: Microscopy
$100 Answer
Which microscope would you choose to view
the cellular contents of a microbe in its natural
state?
a.
b.
c.
d.
compound light microscope
phase-contrast microscope
darkfield microscope
electron microscope
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 2: Microscopy
$200 Question
Which microscope uses an ultraviolet light
source?
a.
b.
c.
d.
compound light microscope
phase-contrast microscope
fluorescence microscope
electron microscope
ANSWER
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Topic 2: Microscopy
$200 Answer
Which microscope uses an ultraviolet light
source?
a.
b.
c.
d.
compound light microscope
phase-contrast microscope
fluorescence microscope
electron microscope
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Topic 2: Microscopy
$300 Question
Which microscope achieves the highest
magnification and the greatest resolution?
a.
b.
c.
d.
compound light microscope
phase-contrast microscope
darkfield microscope
electron microscope
ANSWER
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© 2013 Pearson Education, Inc.
Topic 2: Microscopy
$300 Answer
Which microscope achieves the highest
magnification and the greatest resolution?
a.
b.
c.
d.
compound light microscope
phase-contrast microscope
darkfield microscope
electron microscope
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 2: Microscopy
$400 Question
Which microscope is especially useful in
studying the surface structures of intact cells
and viruses?
a.
b.
c.
d.
transmission electron microscope
scanning electron microscope
phase-contrast microscope
darkfield microscope
ANSWER
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© 2013 Pearson Education, Inc.
Topic 2: Microscopy
$400 Answer
Which microscope is especially useful in
studying the surface structures of intact cells
and viruses?
a.
b.
c.
d.
transmission electron microscope
scanning electron microscope
phase-contrast microscope
darkfield microscope
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 2: Microscopy
$500 Question
What microscope should be used to view
three-dimensional images?
a.
b.
c.
d.
differential interference contrast microscope
darkfield microscope
phase-contrast microscope
transmission electron microscope
ANSWER
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© 2013 Pearson Education, Inc.
Topic 2: Microscopy
$500 Answer
What microscope should be used to view
three-dimensional images?
a.
b.
c.
d.
differential interference contrast microscope
darkfield microscope
phase-contrast microscope
transmission electron microscope
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 3: Electron Microscopy
$100 Question
Objects smaller than about 0.2 micrometers,
such as viruses, must be examined with a(n)
a.
b.
c.
d.
compound microscope.
electron microscope.
phase-contrast microscope.
simple microscope.
ANSWER
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© 2013 Pearson Education, Inc.
Topic 3: Electron Microscopy
$100 Answer
Objects smaller than about 0.2 micrometers,
such as viruses, must be examined with a(n)
a.
b.
c.
d.
compound microscope.
electron microscope.
phase-contrast microscope.
simple microscope.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 3: Electron Microscopy
$200 Question
In electron microscopy, the specimen is placed
on a
a.
b.
c.
d.
copper mesh grid.
glass slide.
plastic slide.
magnesium mesh grid.
ANSWER
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© 2013 Pearson Education, Inc.
Topic 3: Electron Microscopy
$200 Answer
In electron microscopy, the specimen is placed
on a
a.
b.
c.
d.
copper mesh grid.
glass slide.
plastic slide.
magnesium mesh grid.
BACK TO GAME
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Topic 3: Electron Microscopy
$300 Question
Shadow casting is useful
a. for the study of the very smallest specimens, such as virus
particles.
b. to provide a general idea of size and shape of the
specimen.
c. to increase the electron opacity of the surrounding field.
d. to enhance the contrast between the specimen and the
background.
ANSWER
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© 2013 Pearson Education, Inc.
Topic 3: Electron Microscopy
$300 Answer
Shadow casting is useful
a. for the study of the very smallest specimens, such as virus
particles.
b. to provide a general idea of size and shape of the
specimen.
c. to increase the electron opacity of the surrounding field.
d. to enhance the contrast between the specimen and the
background.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 3: Electron Microscopy
$400 Question
In a scanning electron microscope, objects are
generally magnified
a.
b.
c.
d.
100 to 100,000x.
100 to 10,000x.
1000 to 100,000x.
1000 to 10,000x.
ANSWER
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© 2013 Pearson Education, Inc.
Topic 3: Electron Microscopy
$400 Answer
In a scanning electron microscope, objects are
generally magnified
a.
b.
c.
d.
100 to 100,000x.
100 to 10,000x.
1000 to 100,000x.
1000 to 10,000x.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 3: Electron Microscopy
$500 Question
In a transmission electron microscope, objects
are generally magnified
a.
b.
c.
d.
1000 to 100,000x.
1000 to 10,000x.
10,000 to 100,000x.
10,000 to 1,000,000x.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 3: Electron Microscopy
$500 Answer
In a transmission electron microscope, objects
are generally magnified
a.
b.
c.
d.
1000 to 100,000x.
1000 to 10,000x.
10,000 to 100,000x.
10,000 to 1,000,000x.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Preparing Smears for Staining
$100 Question
Why are smear preparations fixed?
a. to kill and color the microorganisms
b. to color the microorganisms and fix them to the
slide
c. to kill the microorganisms and fix them to the
slide
d. to kill and color the microorganisms and fix them
to the slide
ANSWER
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© 2013 Pearson Education, Inc.
Topic 4: Preparing Smears for Staining
$100 Answer
Why are smear preparations fixed?
a. to kill and color the microorganisms
b. to color the microorganisms and fix them to the
slide
c. to kill the microorganisms and fix them to the
slide
d. to kill and color the microorganisms and fix them
to the slide
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Preparing Smears for Staining
$200 Question
Basic dyes are _________ ions and are
_________ to most types of bacteria.
a.
b.
c.
d.
positive; attracted
negative; attracted
positive; not attracted
negative; not attracted
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Preparing Smears for Staining
$200 Answer
Basic dyes are _________ ions and are
_________ to most types of bacteria.
a.
b.
c.
d.
positive; attracted
negative; attracted
positive; not attracted
negative; not attracted
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Preparing Smears for Staining
$300 Question
What is staining?
a. observing shapes, sizes, and capsules of
microorganisms
b. killing the microorganisms and fixing them to the
slide
c. spreading a thin film of material containing
microorganisms on a slide
d. coloring the microorganisms with a dye that
emphasizes certain structures
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Preparing Smears for Staining
$300 Answer
What is staining?
a. observing shapes, sizes, and capsules of
microorganisms
b. killing the microorganisms and fixing them to the
slide
c. spreading a thin film of material containing
microorganisms on a slide
d. coloring the microorganisms with a dye that
emphasizes certain structures
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Preparing Smears for Staining
$400 Question
To stain an organism correctly, one must first
a.
b.
c.
d.
kill the organism.
stain the slide.
prepare a smear.
fix the slide.
ANSWER
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© 2013 Pearson Education, Inc.
Topic 4: Preparing Smears for Staining
$400 Answer
To stain an organism correctly, one must first
a.
b.
c.
d.
kill the organism.
stain the slide.
prepare a smear.
fix the slide.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Preparing Smears for Staining
$500 Question
Distortions of cell size and shape are minimized in
a.
b.
c.
d.
negative staining.
Gram staining.
acid-fast staining.
endospore staining.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 4: Preparing Smears for Staining
$500 Answer
Distortions of cell size and shape are minimized in
a.
b.
c.
d.
negative staining.
Gram staining.
acid-fast staining.
endospore staining.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Staining
$100 Question
What is the mordant in the Gram stain?
a.
b.
c.
d.
iodine
safranin
alcohol-acetone
crystal violet
ANSWER
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© 2013 Pearson Education, Inc.
Topic 5: Staining
$100 Answer
What is the mordant in the Gram stain?
a.
b.
c.
d.
iodine
safranin
alcohol-acetone
crystal violet
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Staining
$200 Question
Flagella are stained with a(n)
a.
b.
c.
d.
carbolfuchsin stain.
malachite green stain.
methylene blue stain.
acid-fast stain.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Staining
$200 Answer
Flagella are stained with a(n)
a.
b.
c.
d.
carbolfuchsin stain.
malachite green stain.
methylene blue stain.
acid-fast stain.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Staining
$300 Question
Capsules are stained with a(n)
a.
b.
c.
d.
negative stain.
Gram stain.
Schaeffer-Fulton stain.
acid-fast stain.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Staining
$300 Answer
Capsules are stained with a(n)
a.
b.
c.
d.
negative stain.
Gram stain.
Schaeffer-Fulton stain.
acid-fast stain.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Staining
$400 Question
Endospores are stained with a(n)
a.
b.
c.
d.
negative stain.
Gram stain.
Schaeffer-Fulton stain.
acid-fast stain.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Staining
$400 Answer
Endospores are stained with a(n)
a.
b.
c.
d.
negative stain.
Gram stain.
Schaeffer-Fulton stain.
acid-fast stain.
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Staining
$500 Question
What color are gram-negative bacteria at the
completion of the Gram staining process?
a.
b.
c.
d.
pink
purple
colorless
brown
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
Topic 5: Staining
$500 Answer
What color are gram-negative bacteria at the
completion of the Gram staining process?
a.
b.
c.
d.
pink
purple
colorless
brown
BACK TO GAME
© 2013 Pearson Education, Inc.
FINAL ROUND Question
The appearance of Mycobacterium leprae
after they are stained with an acid-fast stain is
a.
b.
c.
d.
purple.
red.
colorless.
brown.
ANSWER
BACK TO GAME
© 2013 Pearson Education, Inc.
FINAL ROUND Answer
The appearance of Mycobacterium leprae
after they are stained with an acid-fast stain is
a.
b.
c.
d.
purple.
red.
colorless.
brown.
BACK TO GAME
© 2013 Pearson Education, Inc.