Transcript 슬라이드 1 - Scientific Committee on Antarctic Research

PAMC: Polar and Alpine Microbial Collection
Yung Mi Lee1, Cheng-Dae Choe2, Jeong-Han Yim1, Hong Kum Lee1, and Soon Gyu Hong1
1 Korea
2
Polar Research Institute
BRN Science Co.
Needs for Database
▣ Management and sharing of bio-resources from polar areas
▣ Sharing biodiversity information with national and international organization
www. iobis.org
Culture Collections
Total
No. of Culture collections
No. of Cultures
585
1,725,498
755,818 of them are Bacteria.
505,521 of them are Fungi.
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www.wfcc.info
PAMC: Polar and Alpine Microbial Collection
▣ pamc.kopri.re.kr
▣ Culture Collection to maintain and distribute microorganisms
originated from polar areas
▣ Approximately 500 strains are available for delivery
and the number will increase gradually
Polar and Alpine Microbial Collection
Polar and Alpine Microbial
Collection
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About PAMC
Data Management of PAMC
Public databases
• Data download (GenBank Taxonomy etc)
Public users
Contributors
Deposit of
resources
Delivery of
resources
• Metadata upload (AMD)
• Original data transfer (EBA, SCAR-Marbin etc)
Search
PAMC
Data
download
Public users
Curators
• Long-term preservation of bio-resources
• Identification
• Characterization
• Data updates
Strains
▣ Microbial cultures
 Bacteria: 6,400 strains
 Fungi: 300 strains
▣ Preservation
 Freezing using Glycerol (-180C) and DMSO (-80C)
 Freeze-dry (4C)
Preservation of strains
Summary of PAMC Strains
Habitats of strains
Biofilm
Algae
Lichen
Debris
(animal/plant)
Animal
Moss
8.50%
3.60%
1.30%
Abiotic sample
1.20%
16%
Sediment
Soil
Water
Cryoconite
Rock
Glacier/Snow
0.80%
0.80%
Locality distribution of strains
Austria (Alps) (8%)
Mongol (1%)
Okhotsk sea (1%)
Biotic sample
84%
Phylum composition of identified strains
King George Island (23%)
Actinobacteria, 188
Adeli Island (0.5%)
10%
43.70%
20.50%
9.70%
8.50%
1.10%
0.30%
23%
Antarctica
Gammaproteobacteria,597
Arctic
Firmicutes, 265
Other
67%
Kara sea (40%)
Svalbard (21%)
Barents sea (3%)
Bacteroidetes,191
Betaproteobacteria,49
Alphaproteobacteria,50
Composition of Identified Strains
Actinobacteria
16 Genera
188 Strains
Alphaproteobacteria
16 Genera
50 Strains
Bacteroidetes
25 Genera
191 Strains
Betaproteobacteria
4 Genera
49 Strains
Firmicutes
11 Genera
265 Strains
Gammaproteobacteria
18 Genera
597 Strains
No. of Strains
Physiological Characteristics
3000
2500
2000
Score 1
Score 2
1500
Score 3
1000
Score 4
500
0
▲ Growth on the 96 well plate
No. of Strains
4℃
10℃
15℃
20℃
30℃
Growth Temperature
1000
500
800
400
600
Score 1 300
Score 2
400
Score 3 200
Score 4
200
37℃
100
0
0
▲ Formation of hydrolytic zone on the 96 well plate
25℃
10℃
20℃
10℃
20℃
Protease Lipase
10℃
20℃
Exopolysaccharide
Sample Database
Strain Database
PAMC - Searching
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Pseudoalteromonas
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PAMC – Advanced Searching
Polar and Alpine Microbial Collection
Polar and Alpine Microbial Collection
Searching Keywords in PAMC
Polar and Alpine Microbial Collection
Taxonomy
Geography
Habitats
Characteristics
Searching for microbial strains using various keywords such as taxonomy, geography,
habitats and physiological characteristics are available in PAMC.
Vision of PAMC
▣ To maintain strains for the sustainable use
▣ To supply authentic strains for polar sciences
▣ To expand the service materials: fungal culture, gDNA,
metabolite extract, etc
▣ PAMC website will be available on 1 October