Transcript Palm-Cycler Introduction

Combining robust
engineering,
advanced performance and
a palmtop PC interface for
supreme user-friendly flexibility
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Why a Palmtop PC Interface?
Familiar Windows operating system
Allows total flexibility in cycle programming
Free software upgrades via web download
SD memory cards for program transfer & backup
Computer supports advanced software
(for auto-calibration, primer design, virtual runs, etc)
Intuitive graphical user interface with large back-lit,
color, touch-sensitive screen
In-built palm calendar software makes a perfect
lab booking system!
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Thermal Control
Eight Peltier array
Four separate sensors
Sensor distribution allows
Peltier devices pairs to be
controlled independently via
feedback loops to computer
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Thermal Gradient Cycling
When enabled, a programmable
gradient from 1°C to 24°C can
be setup from left to right on the
Palm-Cycler
8 Peltiers and 4 sensors enable
a true linear gradient to be
established
Gradient technology allows the
user to quickly identify the best
possible set of annealing
conditions in one run when
optimizing cycling conditions
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Logging and Graphing information
A real-time temperature graph provides
information on temperature across
block during cycling
The Blue line shows set temperature
and the Red line actual temperature
Four sensors across the block enables
accurate indication of the temperature
in each well across all 12 columns
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Heated Lid (Hot Bonnet) Technology
Heated lid keeps the void air volume in the
tube hotter than the reaction
This prevents evaporation of the reaction mix
and condensation within the tube
No oil or wax condensation barrier is needed
PCR Tube
Reaction Mix
Aluminum Block
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Programming the Palm-Cycler
From the Cycling
screen, simply:
2. Set Temp.
1. Set Time
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3. Set Repeats
Touchdown Applications
Touchdown programming can reduce non-specific product formation
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Long Range Applications
In Long Range programming,
the extension step of an
amplification cycle is
automatically time incremented
This provides the DNA
polymerase more time to
complete strand synthesis in
later cycles where this
becomes limiting
Useful for targets greater than
about 1 Kb in length
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Limit Rate Feature
Thermal transition times can be precisely
controlled by the limit rate option
This allows the user to specify a heating
or cooling rate over time
For example, you may wish to heat from
55°C to 95°C over 15 minutes (or vice versa)
Used during cycling protocols only
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Hold Lists
The hold list allows you to create
temperature holds during the program
at a defined Cycle, Repeat, Number,
or Step
When running a program with this
feature active, the unit will hold the
temperature at the programmed point
and a beeping sound will be emitted
Select Continue in the top right hand
corner of the display to proceed
Useful if you need to remove tubes or
add reagents at a particular point
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Oligo Calculator
The powerful palm computer allows the
inclusion of advanced functions such
as an oligonucleotide calculator
Used to calculate various useful
oligo information such as:
 %GC
 Molecular weight
 Temperature of melting (TM),
 Optical density (OD)
 Bond information (for detecting
Primer Dimers, Palindromes etc)
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Auto-Calibration
Utilizing the calibration mode within
the Palm-Cycler means thermal
accuracy should never ‘Drift’
throughout the life of the unit
An external thermal couple can be
used to measure block temperature
These readings are fed back into the
calibration settings in the units
memory
Any disparity between readings is
automatically compensated by the
Palm-Cycler to ensure accurate
temperature control
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Active-cooled block (range 4°C to 99°C)
8 Peltiers and 4 platinum sensors
True linear gradient capability (1°C to 24°C)
Intuitive Palm computer interface
Easy programming and backup
Advanced cycling options
(Touchdown, Long Range, Ramp Rate, Hold Lis,t etc)
Advanced functions
(graphing interface, auto-calibration, oligo calculator etc)
Built tough to last
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Offices
Brisbane Australia
Corbett Robotics Pty Ltd
42 McKechnie Drive
Eight Mile Plains, QLD 4113
T +61 7 3841 7077
F +61 7 3841 6077
Sydney Australia
Corbett Research Pty Ltd
14 Hilly Street
Mortlake, NSW 2137
T 1 800 803 915 (Toll free)
T +61 2 9736 1320
F +61 2 9736 1364
E-mail [email protected]
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United Kingdom
Corbett Research UK Limited
Unit 296 Cambridge Science Park
Milton Road, Cambridge CB4 0WD
T +44 (0)1223 424 288
F +44 (0)1223 424 144
USA
Corbett Robotics Inc
185 Berry Street, Suite 5200
San Francisco, CA 94107
T +1 866 380 1166 (Toll free)
T +1 415 348 1166
F +1 415 348 1177
Web www.corbettlifescience.com
All slides  2007 Corbett Life Science. All rights reserved