Transcript Bacterial Media & Culture

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From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Image: Compound microscope objectives, T. Port
Laboratory
Exercise 2b
Media & Culture
Examining Colony Morphology &
Interpreting Specialized Media
From the Virtual Microbiology Classroom on ScienceProfOnline.com
Images: Mannitol Salt, MacConkey;s & Arm plate, T. Port
What am I going to learn from Lab Topic #2?
Media & Culture
•
You will practice microbial collection
techniques.
•
Define and use aseptic technique in
microbial culture and media
preparation.
•
Use enriched, complex, selective &
differential media to culture microbes.
•
Describe colony morphology and its
relationship to microbial identification.
•
Interpret results of microbial growth
on various culture media.
•
Understand the ubiquitous nature of
microbes.
From the Virtual Microbiology Classroom on ScienceProfOnline.com
Image: Chimp brain in a jar, Gaetan Lee
Specialized
Bacterial Growth
Media:
McConkey’s, Mannitol Salt
& Blood Agar
Last week you obtained samples of bacteria from the environment and
from your body. You plated those samples on TSY and on
specialized selective & differential media.
McConkey’s = lighter, purplish-pink
Mannitol Salt = orangish-pink
Blood Agar = very dark red
From the Virtual Microbiology Classroom on ScienceProfOnline.com
Image: McConkey’s, Mannitol Salt &
Blood Agar specialized media, T. Port
MacConkey's
(MAC)
Q: Is MacConkey’s selective? Explain.
Q: Is MacConkey’s differential? Explain.
Watch
VIDEO:
How to Interpret
MacConkey’s Agar
(MAC)
From the Virtual Microbiology Classroom on ScienceProfOnline.com
Image: McConkey’s growing Salmonella on
the left, and E. coli on the right, T. Port
Mannitol Salt
(MSA)
Q: Is Mannitol Salt selective? Explain.
Q: Is Mannitol Salt differential? Explain.
Watch
VIDEO:
How to Interpret
Mannitol Salt Agar
(MSA)
From the Virtual Microbiology Classroom on ScienceProfOnline.com
Images: Sterile Mannitol Salt Agar, Positive & negative
differential reaction on Mannitol Salt Agar, T. Port
Blood agar (BAP)
Q: Is Blood agar selective? Explain.
Q: Is Blood Agar differential? Explain.
Watch
VIDEO:
How to Interpret
Blood Agar (BAP)
From the Virtual Microbiology Classroom on ScienceProfOnline.com
Images: Beta-hemolysis, Alpha-hemolysis
and a sterile plate of Blood Agar, T. Port
Microbial Colony Morphology
From the Virtual Microbiology Classroom on ScienceProfOnline.com
Images: Arm plate, TSY with sample from dish washer,
MacConkey’s with variety of colonies, all by T. Port
Microbial Colony Morphology
Record Results on Lab Report #2
• Question #5: Describe results from touch
plates and arm plate (TSY).
a. What is the difference between touch plates 1, 2 and 3?
b. Is this what you expected?
• Question #6: Record and interpret results
from nasal swab plates (TSY and Mannitol Salt).
a. Did any of the colonies turn the Mannitol Salt medium yellow?
b. What would it mean if they did?
• Question #7: Record and interpret results
from throat swab plate (Blood agar).
a. Do you see Alpha hemolysis? Beta hemolysis? Gamma hemolysis?
b. Which hemolysis pattern is bad news?
• Question #8: Record and interpret results of
environmental samples, for each quadrant 1 – 4
(TSY and MacConkey’s).
a. Are MacConkey colonies pink or colorless?
b. What does it mean if they are pink?
From the Virtual Microbiology Classroom on ScienceProfOnline.com
Images: Arm plate, Mannitol Salt, Blood
agar & MacConkey’s, all by T. Port
When you’re done
with your
environmental and
clinical plates, put
them in the discard
bin at back of lab.
From the Virtual Microbiology Classroom on ScienceProfOnline.com
Confused?
Here are links to fun resources that further
explain microbiology media & culture:
•
Media & Culture Laboratory Main Page on the Virtual
•
“Germs”, music by Weird Al Yankovic. Video by RevLucio.
•
Normal Flora
•
How to Interpret: MacConkey’s (MAC), Mannitol Salt
(MSA) and Blood Agar (BAP) videos from Science Prof Online.
•
How to Pour Bacterial Growth Media into Petri Dishes,
•
Bacterial growth
•
Microbial Growth & Metabolism Main Page on the Virtual
•
E. coli population growth time lapse video.
Microbiology Classroom of Science Prof Online.
webpage, by Douglas F. Fix. Interactive page where you
can select an area of the body and learn which normal flora typically
colonize that location.
video from Science Prof Online.
video and narration, YouTube, Dizzo95..
Microbiology Classroom of Science Prof Online.
(You must be in PPT slideshow view to click on links.)
From the Virtual Microbiology Classroom on ScienceProfOnline.com
Are microbes intimidating you?
Do yourself a favor. Use the…
Virtual Microbiology
Classroom (VMC) !
The VMC is full of resources to help you succeed,
including:
•
•
•
practice test questions
review questions
study guides and learning objectives
You can access the VMC by going to the Science Prof Online website
www.ScienceProfOnline.com
Images: Staph, Giant Microbes; Prokaryotic cell, Mariana Ruiz