Transcript Evaluating Benefits of Pond-Rearing High

Ohio Aquaculture Research and Development
Integration Program (OARDIP) - Update
The Ohio State University
Han-Ping Wang (PD)
Geoff Wallat (Co-PD)
Laura Tiu (Co-PD)
Macdonald Wick (Co-PD)
Five sub-programs and one lab
O’GIFT (Ohio Genetic Improvement of Farmedfish Traits) Program - Piketon
Developing Applied Techniques for Aquaculture
(DATA) Program - Piketon
Bowling Green Aquaculture Program (BGAC) –
Bowling Green
Fish muscle growth and nutrition program –
Animal Science
Aquaculture Technology Transfer (AT2) –
Piketon and Bowling Green
New Aquaculture Genetics and Breeding Lab
(AGBL) at Piketon
OARDIP Structure
The Ohio State University
Piketon Aquaculture
Center
OARDIP
GIFT
DATA
Bowling Green
Aquaculture Center
BAIT
RAS
Department of
Animal Science
School of Natural
Resource
Fish Muscle Growth and Nutrition
AT2 (Aquaculture Technology Transfer) program
Aquaculture Industry
OARDIP OUTCOMES
O’GIFT - Integrated
genetic
improvement of yellow perch
broodstock through MAS
Plus WI & NE
1. Completed three studies on
stock evaluation
Study 1: Testing early Growth of Different Strains at 22 O C
Study 2: Evaluating long-term Growth of different strains at
ambient temperature (fry to harvest size)
Study 3: Quantifying genetic variation within and among
broodstock groups
Weight (g)
Early Growth of Different Strains at 22 oC
18
16
14
12
10
a
a
a
a
b
c
8
6
4
2
0
NC
PA
OH
WI
NE
NY
0
2
4
6
Week
8
10
Long-term growth of strains at ambient temperature
(Well water:10 – 22 C)
140
NC04
100
80
60
40
20
Nursery + Feeding training
Weight (g)
120
a
Experiment period
OH05
NY05
cc
WI05
NE05
0
0
a
b
b
PA04
2
4
6
z
z
y
8
10
12
14
16
18
Months
MS for above two experiments is in preparation
Quantifying genetic variation among
broodstock groups
NC and OH
populations
have the most
detectable
genetic
variation
All population
pairs show
significant
differentiation
There is a low
possibility of
inbreeding
when
crossing
strains.
Chance of
inbreeding
within any
strain is high
Aquaculture 271:142–151.2007
2. Genetic tool development
Developed 93 microsatellite
markers for YP in 2006, and
published in Molecular Ecology
Notes 7: 600-603, 2007
122
Developed 21 additional
microsatellite markers in 2008,
which was published in
International Journal
of Molecular Science 10: 18 –
27, 2009
100 more markers are being
developed for genetic mapping
3. Technique for family identification
established
300
Frequency
250
Locus YP17
200
150
100
50
Optimized 8 best markers for family
identification
0
223
229
232
235
238
241
253
301
335
300
Frequency
250
Locus YP49
200
150
100
50
0
111 117 120 123 126 129 132 138 141 144 147 150 153 156
250
200
Frequancy
Using four microsatellite markers
allowed us to reliably assign 97.9% of
progeny back to one of 17 families
226
Locus YP60
150
100
50
192 196 200 204 208 212 216 220 224 228 232 236 244 248
Frequency
Using 8 markers, 98.4% of offspring
could be correctly allocated to one of
30 families
0
160
140
120
100
80
60
40
20
0
Locus YP109
129
135
144
150
156
165
Allele
171
180
186
195
4. Method for Genetic relatedness
analysis developed
Analysis Synopsis:
Full sib:
Half sib:
Cousin:
Analysis Requested: RELATEDNESS
0.50
0.25
0.06
North Carolina (NC) vs. Ohio (OH)
Number of Individuals: 135
Tag: 15D2D BL22D V8VQD9T867 JKNKGP242D 1641D QJJGH 56TGH FVVADB7OR1 CCD0HMH8CG
Tag:
NC-1 NC-2 NC-3 NC-4 NC-5 NC-6 NC-7 NC-8 NC-9 NC-10 NC-11 NC-12 NC-13
6UCOV OH-1
0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.00 0.31 0.27 0.00 0.00 0.00
VU2QD OH-2
0.02 0.01 0.63 0.02 0.02 0.00 0.05 0.65 0.00 0.00 0.01 0.01 0.65
CDSOH OH-3
0.00 0.00 0.04 0.00 0.00 0.00 0.00 0.06 0.00 0.00 0.00 0.00 0.06
5FUOH
OH-4
0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.30 0.25 0.00 0.00 0.00
BAKID
OH-5
0.36 0.00 0.00 0.00 0.36 0.00 0.00 0.00 0.29 0.25 0.14 0.00 0.00
A8GPD OH-6
0.15 0.14 0.05 0.00 0.15 0.00 0.18 0.07 0.36 0.31 0.00 0.00 0.07
7JIOH
OH-7
0.00 0.00 0.00 0.00 0.00 0.00 0.59 0.00 0.00 0.00 0.00 0.00 0.00
Q0UR1
OH-8
0.11 0.34 0.26 0.05 0.11 0.25 0.13 0.03 0.31 0.51 0.06 0.06 0.02
SOICG
OH-9
0.00 0.00 0.00 0.00 0.00 0.00 0.30 0.00 0.37 0.34 0.00 0.00 0.00
PDI87
OH-10 0.00 0.00 0.01 0.00 0.00 0.00 0.00 0.03 0.00 0.00 0.00 0.00 0.03
1TTID
OH-11 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.36 0.00 0.00
TT1G4
OH-12 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.28 0.25 0.00 0.00 0.00
1P02D
OH-13 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
AMQ9D OH-14 0.00 0.00 0.41 0.00 0.01 0.00 0.01 0.43 0.00 0.00 0.01 0.02 0.43
77IGV
OH-15 0.02 0.01 0.63 0.02 0.02 0.00 0.05 0.65 0.00 0.00 0.01 0.01 0.65
CR7ID
OH-16 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.36 0.33 0.00 0.00 0.00
ER89D
OH-17 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
AJG8H
OH-18 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.00 0.00 0.00 0.00 0.01
5C1G7
OH-19 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
I4F6U
OH-20 0.30 0.03 0.00 0.00 0.32 0.00 0.05 0.25 0.17 0.14 0.17 0.00 0.26
L8C6U
OH-21 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
HTIQD
OH-22 0.00 0.00 0.11 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.11
EJPO7
OH-23 0.00 0.00 0.40 0.00 0.00 0.00 0.00 0.41 0.00 0.00 0.00 0.00 0.42
5. Two generations of improved
fish are created
Founder
1st selected
generation
2nd selected
generation
3rd selected
generation
2004 YC (300)
40Fa/18,000fish
2005 YC (200)
34Fa/24,000fish
2006 YC (700)
30Fa/16,000fish
2007 YC (1000)
Line 1 Line 2
3 Lines
2008 YC
2009 YC
2 Lines
3 Lines
2010 YC
2011 YC
6. Two on-station tank tests completed
for improved lines from 06 YC
14
a
12
Improved line 1 39.8% faster
Improved line 2 18.3% faster
Control
Weight (g)
10
b
c
8
6
4
2
0
D0
D21
D42
D63
D84
D105
D126
Days
Early growth of the improved lines vs control
(1.8’ round tank, 4 reps, ~22 C)
On-station tank test for improved lines
120
100
Weight (g)
a
a
Improved line 1
(42% faster)
Improved line 2
(31% faster)
Control
80
b
60
40
20
0
J
S
N
J
A
J
A
O
D
July - Dec, 06 -07
Long-term growth of the improved lines vs control
(3’ tank, 3 reps, 10-22 C)
7. Two tests completed for improved lines
from 07 year class
Piketon Pond
Farm RAS
30
35
30
Weight (g)
25
20
07P10
07P11
07P12
07CON
32.8%
30.6%
18.0%
b
15
a
25
ab
Weight (g)
a a
20
15
b
07P11 (28%faster)
Control
10
10
5
5
0
0
July
Oct
July
Month
Oct
Month
Growth of improved lines from 07 YC vs controls
8. Two studies on G x E
interactions completed
.
• Evaluation of relative growth performance and
genotype by environment effects for cross-bred
yellow perch families reared in communal ponds
using DNA parentage analyses Aquaculture
Research 40: 1363 - 1373
• DNA marker based estimation of effects of family
by tank interactions on early growth performance
of yellow perch reared in single family tanks versus
in multi-family tanks. Journal of Fish Biology
(Accepted)
9. Four F2 mapping families created
Family 1
Family 2
…….3………4
.
♀♂
tagged grandparents
F1
F2
♂♀ ♂♀ ♂♀ ♂♀ ♂♀……
tagged parents
Offspring:
|||||||| |||||||| |||||||| |||||||| |||||||
tag
communally reared in tank/pond
genotyping
Genetic mapping
♀♂
tagged grandparents
♂♀ ♂♀ ♂♀ ♂♀ ♂♀……
tagged parents
Offspring
|||||||| |||||||| |||||||| |||||||| |||||||
tag
communally reared in tank/ pond
genotyping
Genetic mapping
Detecting and mapping quantitative trait loci (QTL)
using the F2 populations is under way
OARDIP OUTCOMES
O’GIFT - Develop mass production
technology of genetically male
bluegill populations
Why: Bluegill Growth: ♂ > ♀
(Hayward and Wang, 2006)
160
Body weight (g)
Experiment 2
Experiment 1
140
120
BG-M
BG-F
HY-M
HY-F
100
80
60
40
20
0
0
25
50
75 100
0
25 50 75 100 125 150 175 200 225
Days
May 00
Aug 00
Mar 01
10. Completed a study on gonadal
sex differentiation in bluegill
Sex differentiation is more related size than age
The critical period of sex determination in bluegill occurs
between 13.2 -16.0 mm TL
sex differentiation is distinguishable in most fish at 21.028.0 mm TL
The work was published in Aquaculture, 2009
11. A first linkage map of BG was
constructed for ♂ and ♀
LG2 116cM (10)
LG1 345.3cM (19)
LG9 62.6cM (8)
LG3 106.7cM (8)
LG10 54.5cM (4)
LG4 89.7cM (9)
LG11 43.4cM (9)
LG5 81.6cM (3)
LG6 76.9cM (6)
LG12 38.6cM (3)
LG13 32.6cM (3)
LG7 75.8cM (5)
LG14 31cM (3)
LG8 72.6cM (6)
LG15 28.2cM (6)
Published by Aquaculture International, 2009
OARDIP OUTCOMES
O’GIFT - Stock evaluations of LMB
for genetic improvement
Seven strains have been
obtained for its selective breeding
We are developing genetic tools
or markers for this species
Stock evaluation on genetic
variation and growth is underway
OARDIP OUTCOMES
Determine the efficacy of duoculture of yellow
perch and bluegill in increasing growth rate
Manuscript has been submitted to
Journal of Fish Biology
12. Completed two experiments
Two
experiments
were conducted
under two
temperature
conditions
One was
conducted in
cages in a pond
One in tanks
Each experiment
consisted of three
groups, each with 3
replicates.
• yellow perch in
monoculture
• bluegill in monoculture
• yellow perch + bluegill in
duoculture
-means of
22.0C and
26.5C
Body weight (g)
Experiment 1 – temp. 26.5 C
70
60
50
40
30
20
10
0
BGD
BGM
ns
Body weight (g)
D0
70
60
50
40
30
20
10
0
*
D40
*
D80
*
62.5% faster
*
D120
D150
ns
YPD
YPM
ns
*
D0
D40
D80
Days
D120
D150
Experiment 2 – temp. 22.0 C
30
31.2% faster
Body weight (g)
25
YPD
20
YPM
15
10
*
5
0
D0
D21
D42
*
D63
*
D84
*
*
D105
D126
Day
Body weight (g)
10
8
BGD
6
BGM
*
D21
D42
4
2
0
D0
D63
D84 D105 D126
Journal of Fish Biology (In review)
OARDIP OUTCOMES
Establish mostly-male groups of bluegill by size
grading and evaluate their growth potential
vs. mixed-sex groups
Paper was
published by
North American
Journal of
Aquaculture
13. A practical procedure to establish mostly-male
bluegill groups via size grading developed
Group
Male
percentage
Survival
(%)
Marketable
fish (%)
Top 25%
75.4
96.0
46.3
Top 50%
69.7
90.6
28.3
Mixed
50.0
90.6
12.7
Weight gain of three groups
70
mixed
60
a
Weight gain (g)
25%
50
a
50%
40
b
ab
ab
b
b
b
30
20
10
a
a
a a
0
8
24
32
44
Week
The established male group was able to gain 61.0% more weight than
the regular mixed-sex control.
OARDIP OUTCOMES
Bowling Green Aquaculture
Program
14. Completed Project “Duoculture
of largemouth bass and yellow
perch in 3 RAS systems”
Woodside Farm (WS)
Fish Farm (FF)
Aqua-cube (AC)
Mean Weight Gains
Largemouth Bass
250
Grams
200
150
LMB -FF
LMB -WS
100
LMB -AC
50
0
Mar 07
Jan 08
Apr 08
Yellow Perch
300
250
200
YP - FF
Grams 150
YP-WS
100
YP-AC
50
0
Mar 07
Jan 08
Apr 08
15. Completed Aquaponics Trials
•
Variety trials with use of fish culture tank effluent in
half the plantings v. normal hydroponic fertilization
Conducted Trials with:
Cherry and Beefsteak tomatoes (2 varieties); Peppers; Leaf
lettuce
Variety of herbs including chives, basil
Cucumbers (4 varieties); Zucchini and Eggplant (3 varieties)
16. Developed Baitfish Culture
Techniques
Established
out of season
spawning
technique
Completed
Baitfish
Retailers
Survey Study
Completed
indoor baitfish
culture
systems for
growout trials
Completed
live feeds lab
and first feeds
research
system
installation
Golden Shiner
Spotfin Shiner
OARDIP OUTCOMES
Muscle Biology and Nutrition
Program
I.
II.
III.
Identify the gene products associated with muscle
growth in Yellow Perch
Isolate and propagate skeletal muscle satellite
cells from Yellow Perch
Examine the influence of wheat gluten based
diets augmented with dietary lysine on Yellow
Perch growth and fecundity
17. Developed a general
system to identify the gene
products associated with
biological variables in yellow
perch
Reddish et al.
Proteomics 2008
Electrophoresis of muscle proteins
Trout
18. Established satellite
cell lines and
characterized the in
vitro gene expression
patterns of yellow
perch
Dodson, M. et al.
Cytotechnology 2008
Yellow Perch
19. Completed Project “The effect of
lysine-supplemented wheat gluten –
based diet on yellow perch”
Wheat-gluten based diets
supplemented with lysine could
be a good source of protein to
replace fish meal in diets for
yellow perch.
120
100
26
-Lys small fish
+Lys
-Lys large
small
fish fish
+Lys large fish
24
a
22
b
80
Blood
sampling
60
c
Water temperature (oC)
Lysine, as the first limiting amino
acid, directly affects the growth
and plasma free amino acid pool
in yellow perch.
Mean weight of fish (g)
Growth of yellow perch fed –Lys and +Lys diets
20
18
16
d
40
14
12
10
20
8
0
6
Oct 2006Nov
Jul
Mar May 2007
Sep
Dec
Kwasek et al. 2009a, Aquaculture (in review)
20. Completed Project “Wheat gluten based
diets impacts on fecundity in Yellow Perch”
The condition factor and percentage of robust females from the (+) Lys
group was significantly greater than fish fed (-) Lys diets (P < 0.05)
The percentage of fish with scoliosis was significantly higher in the (-) Lys
group than the (+) Lys group (P < 0.05).
Sperm concentration and sperm motility were significantly higher in the
control and +Lys groups in compared to the (–) Lys group (P < 0.05).
The greatest survival of embryos was observed in (+) Lys females fertilized
by (+) Lys males.
The largest survival had embryos from control and (+) Lys females in
comparison to (-) Lys females.
Kwasek et al. 2009b, Aquaculture (in review)
OARDIP OUTCOMES
Research Facility Establishment
21. Established Aquaculture
Genetics and Breeding Lab at
Piketon


This is the first laboratory of its kind for
aquaculture in the Midwest.
The combination of aquaculture genetics
lab, wet lab and pond facility makes us
unique as a fish breeding station in
the Midwest
22. Established Bowling Green Aquaculture Center
Temperature and Photoperiod
Control
Egg Hatching
Fry Rearing
First Feeds Test System
Egg Collection from Pond
Large-Scale Algal Production
23. Established new broodfish tank
system in Piketon
OARDIP OUTCOMES - EDUCATION
24. Students trained/being trained in aquaculture
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
Kwasek Karolina, Ph.D, Current
Beatrix Beres, Ph.D
Weiji Wang, Ph.D
Zexia Gao, Ph.D
Crescent Cao, Ph.D
John Xu, Ph.D
Joe Sawdy MS, DVM
Sarah Kaiser Sawdy MS
Pamela Davenhill, MS
Karolina Kwasek MS
Andy Nichols BS
Heath Williams BS – 2nd place University Undergrad Research forum
Mital Pandya – 1st place College Undergrad Research forum
Katie Betts - 1st place University Undergrad Research forum
OARDIP Economic
Impacts
The USDA National
Agriculture Statistics
Date in 2007 shows,
Ohio aquaculture value
doubled, from 3.2
million to 6.6 million for
the past three years
(http://www.agcensus.u
sda.gov./Publications/
2007/Full_Report/usv1.
pdf)
OARDIP Scientific Impacts
Journal
Articles
Published
(21)
Publications
in review or
preparation
(6)
Patent (1)
Proceedings
Abstracts
(26)
Patent submission
to the Office of
Technology
Transfer “Multiple
linear regression
proteomics” and
have assigned Tech
ID # 08085F.
Published Journal Articles (21)
Wang, H.P., L. Li, G.K. Wallat, B. Brown, H. Yao, Z. Gao L.G. Tiu, P. O’Bryant, D. Rapp and R. MacDonald.
2009. Evaluation of relative growth performance and genotype by environment effects for cross-bred yellow perch
families reared in communal ponds using DNA parentage analyses. Aquaculture Research, 40: 1363 - 1373.
Wang, H.P., H. Yao, P. O’Bryant, D. Rapp, G.K. Wallat, L.G. Tiu and R. MacDonald. 2009. Family-tank
interactions on early growth performance of yellow perch reared in single family tanks versus mixed-family tanks
as inferred using molecular pedigrees. Journal of Fish Biology (accepted).
Gao, Z., H.P. Wang, D. Rapp, P. O’Bryant, H. Yao, G.K. Wallat, W. Wang, R. MacDonald and L.G. Tiu. 2009.
Gonadal sex differentiation in the bluegill sunfish Lepomis macrochirus and its relation to fish size and age.
Aquaculture, 294:138–146.
Wang, H.P. G.K. Wallat, R. Hayward, P. L.G. Tiu, O’Bryant and D. Rapp. 2009. Establishment of mostly-male
groups of bluegill by size-selection and evaluation of their growth performance. North American Journal of
Aquaculture, 71:216–223.
Zhan, A.B, W. Yao, B. Brown, H.P. Wang, 2009. Isolation and characterization of novel microsatellite markers for
yellow perch (Perca flavescens). International Journal of Molecular Science 10:18 – 27.
Wang , W.J., H.P. Wang, L. Li, G.K. Wallat, L.G. Tiu, H.Yao, and Q.Y. Wang. 2009. A first genetic linkage map of
bluegill sunfish (Lepomis macrochirus) using AFLP markers. Aquaculture International (Accepted).
Gao, Z., H.P. Wang, G.K. Wallat, D. Rapp, P. O’Bryant, H. Yao, R. MacDonald and W. Wang, 2009. Effects of a
nonsteroidal aromatase inhibitor on sex reversal, gonadal differentiation and growth performance of bluegill
sunfish Lepomis macrochirus. Aaquaculture research (In press).
Wang, H.P. Z. Gao, R. Hayward, G.K. Wallat, P. O’Bryant, D. Rapp, H. Yao and L.G. Tiu. 2009. Yellow perch in
duoculture with bluegill: Diminished social interaction costs improve growth rates of both fishes at different
temperatures. Journal of Fish Biology (in review).
Gao, Z., H.P. Wang, H. Yao, L.G. Tiu, W. Wang, R. MacDonald and L.G. Tiu. 2009. No sex-specific markers
detected in bluegill sunfish Lepomis macrochirus by AFLP. Journal of Fish Biology (In press).
Wang, H.P. Z. Gao, B. Beres, J. Ottobre, G.K. Wallat, L.G. Tiu, D. Rapp, P. O’Bryant and H. Yao. 2008. Effects of
estradiol-17 on survival, growth performance, sex reversal and gonadal structure of bluegill sunfish Lepomis
macrochirus. Aquaculture 285: 216–223
Journal Articles (cont.)
Brown, B., H. P Wang, L. Li, G. Chandler, and G.K. Wallat. 2007. Yellow perch strain evaluation I. Genetic
variance of six broodstock populations. Aquaculture 271:142–151.
Li, L. H.P. Wang, C. Givens, and B. Brown. 2007. Isolation and characterization of microsatellites in the yellow
perch (Perca flavescens). Molecular Ecology Notes 7: 600-603.
Gao, Z., W. Wang, K. Abbas , X. Zhou, Y. Yang, J.S. Diana, H.P. Wang, H. Wang, Yang. Li, and Y. Sun. 2007.
Haematological characterization of loach Misgurnus anguillicaudatus: Comparison among diploid, triploid and
tetraploid specimens. Com. Biochem. and Physiol. 147: 1001–1008.
Hayward R.S. and H.P. Wang. 2006. Rearing Male Bluegills Indoors May Be Advantageous for Producing Foodsize Sunfish. Journal of the World Aquaculture Society 37: 496-508.
Wallat, G.K, L. G. Tiu, H. P. Wang, D. Rapp, and C. Leighfield. 2005. The effects of size grading on production
efficiency and growth performance of yellow perch in earthen ponds. North American Journal of Aquaculture 67:
34-41.
Reddish, J. M., N. St-Pierre, A. Nichols, K.G. Church and M. Wick. 2008 Proteomic analysis of proteins associated
with body mass and length in yellow perch, Perca flavescens. Proteomics 2008, 8, 2333–2343
Dodson, M. V., A. Kinkel, J. L. Vierck, K. Cain, M. Wick and J. Ottobre. 2008. Unidentified cells reside in fish
skeletal muscle. Cytotechnology 56:171–178. Wang, H.P, K.J. Wei, H. Yao and B.X. Xiong. 2003. Broodstock
rearing and controlled reproduction of Reeves shad Tenualosa reevesii. Journal of the World Aquaculture Society
34: 308 -318.
Wang, H.P. R.S. Hayward, G. W. Whitledge and S. A. Fischer. 2003. Prey-size preference, maximum size and
consumption rates of redear sunfish feeding on two gastropods common to aquaculture ponds. Journal of the
World Aquaculture Society 34: 379 -386.
Wang, H.P. 2003. Biology, population dynamics and culture of Reeves shad, Tenualosa reevesii. American
Fisheries Society Symposium 35:77-83.
Hayward, R.S. and H.P. Wang. 2002. Inherent growth capacity and social cost in bluegill and hybrids of bluegill
and green sunfish: which fish really grows faster? North American Journal of Aquaculture, 64: 34-46.
Publications
in review/preparation (6)
Han-Ping Wang, Zexia Gao, Weimin Wang, Dean Rapp, Paul O’Bryant, Hong
Yao, Geoff Wallat and Russ MacDonald. 2009. Temperature effects and
genotype-temperature interactions on sex determination in bluegill sunfish
Lepomis macrochirus. Journal of Experimental Zoology (In review)
Reddish, J.M., I. Zapata, M. Wick. Comparison of protein expression patterns in
different strains of Yellow Perch (Perca flavescens) using high resolution 1Dimensional electrophoresis Proteomics (In review)
Kwasek, K., K. Dabrowski, M. Wick. Wheat gluten based diets augmented with
lysine improves muscle growth in Yellow Perch. Aquaculture (In review)
Kwasek, K., K. Dabrowski, M. Wick. Wheat gluten based diets impacts on
fecundity in Yellow Perch. Aquaculture (In review) )
Han-Ping Wang, Zexia Gao, Weimin Wang, Dean Rapp, Paul O’Bryant, Hong
Yao, Geoff Wallat and Russ MacDonald. Sex control and sex determinism study
in bluegill by use of endocrinal sex-reversed female breeders (In preparation)
Xiaojuan Cao, Han-Ping Wang, Hong Yao, Paul O'Bryant, Jacob D. Rapp and
Geoffrey K. Wallat. ESTIMATION OF GENETIC PARAMTERS FOR GROWTHRELATED TRAITS OF YELLOW PERCH WITH ONE-STAGE AND TWOSTAGE SELECTION IN PONDS (In preparation)
Proceedings Abstracts (26)
K. Kwasek, K. Dabrowski, J. Nynca, J.M. Reddish and M. Wick. Influence of lysine deficiency on yellow perch (Perca flavescens)
females reproductive performance and the quality of eggs World Aquaculture Society, Seattle, WA 2009.
Wang, H.P., H. Yao, G.K. Wallat, P. O’Bryant, J. D. Rapp, L.G. Tiu, and B. Brown. 2008. Molecular marker assisted cohort selection
of yellow perch. Proceedings of the XXXI Conference of the International Society for Animal Genetics, 20 – 24 July, 2008,
Amsterdam.
Wang, H.P., G.K. Wallat, P. O’Bryant, J. D. Rapp, H. Yao, L.G. Tiu, L. Li, and B. Brown. 2008. Marker-assisted cohort selection of
yellow perch for improvement of growth rate. Proceedings of the 2008 American Aquaculture Conference. Orlando.
Yao, H, H.P. Wang, G.K. Wallat, L.G. Tiu, P. O’Bryant and J.D. Rapp. 2008. Evaluation of growth performance of different families in
individual and communal tanks using microsatellite tool. Proceedings of the 2008 American Aquaculture Conference. Orlando.
Kwasek K., Dabrowski K., Nynca J., Reddish J.M., Wick M. 2009. The influence of dietary lysine deficiency on yellow perch
maturation and the quality of sperm. Asbtract presented European Aquaculture, Krakow, Poland, 2008.
Betts,K., J.M. Reddish, N.S. St-Pierre, M. Wick. Muscle Proteins Associated with Body Mass of Yellow Perch (Perca flavecens).
CFAES and the Richard J. and Martha D. Denman Undergraduate Research Forum. 2008. Awarded 1st place.
Gao, Z., Wang, H.P. B. Beres, J. Ottobre, G.K. Wallat, L.G. Tiu, D. Rapp, P. O’Bryant and H. Yao. 2008. Effects of estradiol-17 on
survival, growth performance, sex reversal and gonadal structure of bluegill sunfish Lepomis macrochirus. Proceedings of the 2008
American Aquaculture Conference. Orlando.
Wang , W.J., H.P. Wang, L. Li, G.K. Wallat, L.G. Tiu, H.Yao, and Q.Y. Wang. 2008. Genetic linkage mapping and sex-specific
marker locating of bluegill sunfish (Lepomis macrochirus) using AFLP markers. World Aquaculture Society Conference, page 555.
Seoul, South Korea.
Reddish, J. M., N.S. St-Pierre1, K.B. Green-Church, A. D. Nichols, M. Wick. Quantitative whole 1-D fingerprints to identify proteins
associated with body mass in yellow perch Perca flavescens. Abstract presented World Aquaculture Society, Orlando FL, 2008.
Kwasek., K. Dabrowski, M. Wick. Utilization of lysine based diets to enhance growth in Yellow Perch (Perca flacevens). Abstract
presented World Aquaculture Society, Orlando FL, 2008.
Wang, H.P, G. K. Wallat, Li Li, L. G. Tiu, J. D. Rapp, P.O’Bryant, and B.B. Brown. 2007. Integrated genetic improvement of yellow
perch broodstock through marker assisted selection. Proceedings of the 2007 World Aquaculture Society Conference, page 965.
San Antonio.
Li L., H.P. Wang, C.B. Givens, G.K., Wallat, L. G. Tiu and B. L. Brown. 2007. Genetic variation among yellow perch perca
flavescens broodstock groups: effects of spawning and management practices to date. Proceedings of the 2007 World Aquaculture
Society Conference, page 524. San Antonio.
Li Li, H.Yao, H.P.Wang, G.K. Wallat, L.G. Tiu, P. O’Bryant, D. Rapp and B. L. Brown. 2007. Microsatellite-based family identification,
offspring fitness environmental effects in communally reared yellow perch Perca flavescens. . Proceedings of the 2007 World
Aquaculture Society Conference, page 525. San Antonio.
Abstract cont.
O’Bryant, P., H.P. Wang, G. K. Wallat, L. G. Tiu, J.D. Rapp and R. McDonald. 2007. Establishing mostly-male groups of bluegill by size-grading and
evaluation of their growth potential versus mixed-sex groups. Proceedings of the 2007 World Aquaculture Society Conference, page: 670. San
Antonio.
Reddish, J. M., N. St-Pierre, K. Green-Church, A. D. Nichols1, M. Wick. Proteomic analysis of whole muscle fingerprints from yellow perch, Perca
flavescens, and identification of proteins associated with body weight and length. Abstract published and presented at Joint ASAS meeting San
Antonio, TX 2007.
Wang, H. P., G. K. Wallat, L. G. Tiu, J. D. Rapp, P. O'Bryant and C. Leighfield. 2006. Stock Evaluation of Yellow Perch for Genetic Improvement.
Proceedings of the 2007 World Aquaculture Society Conference. Las Vegas.
Wang, H. P, G. K. Wallat, L. G. Tiu, J. D. Rapp, P. O'Bryant and C. Leighfield. 2006. Development of genetically male and mostly-male bluegill
populations. Proceedings of the 2006 World Aquaculture Society Conference. Las Vegas.
Pandya, M. J. M. Reddish, M. Wick Comparison of Protein Expression Patterns in different strains of Yellow Perch (Perca flavescens) by the use of
high resolution 1-dimensional and two-dimensional electrophoresis. Poster presentation to both CFAES and the Richard J. and Martha D. Denman
Undergraduate Research Forums, 2006.
Williams, H., J. M. Reddish, M. Wick. 2006. Purification of Myosin Isoforms from Epaxial Muscle of Yellow Perch (Perca flavecens). Poster
presentation Richard J. and Martha D. Denman Undergraduate Research Forum. 2007. Awarded 2nd place.
Wang, H. P, G. K. Wallat, L. G. Tiu, J. D. Rapp, P. O'Bryant , and C. Leighfield. 2006. Broodstock improvement of yellow perch. Proceedings of the
2006 World Aquaculture Society Conference. Las Vegas.
Rapp, J. D., H. P. Wang, G.K. Wallat, P. O’Bryant, L. G. Tiu and R. McDonald. 2007. Determine the efficacy of duoculture of yellow perch and
bluegill in increasing growth rate and reducing social costs of both species in cages. Proceedings of the 2007 World Aquaculture Society
Conference, page: 753. San Antonio.
Wallat, G.K. Laura G. Tiu and H.P.Wang. 2007. Evaluation of fertilizer regimes in the nursery pond production of juvenile yellow perch Perca
flavescens. Proceedings of the 2007 World Aquaculture Society Conference, page: 958. San Antonio.
Wang, H.P. G.K. Wallat, L.G. Tiu, J.D. Rapp, C.Leighfield, and P. O'Bryant. 2005 Integrated genetic improvement of yellow perch broodstock
through marker assisted selection. 13th International Congress on Gene, Gene families and Isozymes. 2005, Shanghai. Abstract 83.
Wang, H.P. G.K. Wallat, L.G. Tiu, J.D. Rapp, C.Leighfield, and P. O'Bryant. 2005. Establishment of marker assisted breeding program for yellow
perch Perca flavescens to improve broodstock and growth rate. Proceedings of the 2005 World Aquaculture Society Conference. Page 251. New
Orleans.
Joseph C. Sawdy, John M. Reddish, Geoff Wallat, Normand R. St-Pierre, and Macdonald Wick. 2005. Whole muscle 1-D protein fingerprints of
yellow perch Perca flavescens correlate with total body weight and length. Aquaculture America 2005, Abstract 229.
John M. Reddish, Joseph C. Sawdy, Geoff Wallat, Normand R. St-Pierre, and Macdonald Wick. 2005. Further characterization of whole muscle 1-D
fingerprints of yellow perch Perca flavescens associated with body weight and length. Aquaculture America 2005, Abstract 445
Rapp, J. D., G. Wallat, L. Wright, L. Tiu and H.P. Wang. 2005. Effect of feeding frequency on the compensatory growth response of yellow perch
Perca flavescens reared in culture cages. Proceedings of the 2006 World Aquaculture Society Conference. New Orleans.
Wallat, G.K., L.G. Tiu, and H.P. Wang. 2005. Impact of grading on the growth performance, survival, and total yield during year one culture of yellow
perch Perca flavescens in open ponds. Proceedings of the 2005 World Aquaculture Society Conference. Page 298. New Orleans.
Acknowledgements
Funding Agency:
Ohio
Soybean
Council
USDA
Sea Grant