Transcript UV/Vis Spectrophotometers

Buck Scientific, Inc.
Seminar in ANALYTICAL
INSTRUMENTATION for
Spectroscopy & Chromatography:
UV-Vis Spectrophotometry
Ultra-Violet/Visible SPECTROSCOPY (UV-Vis)
Theory & Principle of OPERATION
A TYPE of VIBRATIONAL Spectroscopy,
similar to Infra-Red. A Sample in a Cuvette is
exposed to Light Energy between 190nm. (in the
UV) and 1000nm. (in the VIS). Certain parts of
an Organic Molecule will ABSORB some of this
energy to create PEAKS on a SPECTRUM for
Quantitative (primarily) and Qualitative Analysis
BASIC Spectrophotometer Design
Like AAS instrumentation, the ORIGINAL UV-Vis
Specs were made as DOUBLE-BEAM units to
correct for Noise, Drift and other Instabilities. Over
20 years ago, the well-known leaders in the
Analytical Instrument markets; Beckman & PerkinElmer; began to focus on a line of “Stable-Beam”
SINGLE-BEAM Instruments.
The BUCK/Cecil series employs this optimum design
in our instruments with unique Internal software
Basic SPECTROMETER Design
ALL Analytical Spectrometers have several
BASIC design components in common:
Light Source
Sample “Chamber”
Optical System
Detector
Output
Basic SPECTROMETER Design
ALL Analytical Spectrometers have several BASIC
design components in common:
LIGHT SOURCE: External D2 & W Lamps to prevent
thermal instability within instrument (no fan)
SAMPLE “Chamber”: Standard & Specialty Holders
OPTICS: High-energy Ebert Monochromator
DETECTOR: Choice of low-Noise Photo-Diode or
high-Sensitivity Photo-Multiplier Tube
OUTPUT: Graphics On-Screen Display, Analog, Printer
and Serial RS-232 P-C capabilities
UV/Vis Important Features
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Scan Speed
Resolution
Software Features
Ease of Operation
Data Storage
Customized Calculations
Support & Service
BUCK/Cecil Instruments
The COMPACT,
“Stable-Beam”
construction of the
Single-Beam series
uses the high-quality
Cecil base instrument
and high-performance
components from
BUCK Scientific
BUCK/Cecil Instruments
• Best optical design and highest quality
components resulting in superior specifications:
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Stray Light
Noise
Stability
Baseline Flatness
Accuracy (wavelength & photometric / ABS)
Reproducibility (wavelength & photometric / ABS)
BUCK/Cecil Series
• Series 2000
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Single Beam, Scanning, 4nm bandpass
Optional built-in printer & P-C Interface
Many software options
Most POPULAR Industrial system
Applications: Bio-Quest, Aqua-Quest, FoodQuest series with Prep-Programmed Operations
BUCK/Cecil Series
• Series 3000
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Single Beam, Scanning, 1.8nm bandpass
Optional built-in printer & P-C interface
Many software features (Kinetics, Bio-Assay)
Reflectance option: 3055 w/ Integrating Sphere
Specifically designed to meet ALL of the
required Specifications for USP / BP
Pharmaceutical Assays
BUCK/Cecil Series
• Series 7000/7000+/9000
– Double-Beam, Scanning, some Models with
variable 0.5 / 1.0 / 1.8-2.0 / 4.0nm bandpass
– Standard Printer & P-C interface, Data-Stream
– Many software features (Kinetics, Bio-Assay)
– Reflectance / Fluorescence / Turbidity options
Ideal for the stringent requirements of Academic
and Pharmaceutical Research
Sample Handling Options
• Cuvette - standard cell holder
– Quartz for UV range
– Glass & Plastic for Vis range
– 1-100mm Pathlengths
• Flow Cell - standard or micro flow (50L)
– Uses Sipette Pump
– Optional Automatic Cell Changer
Sample Handling Options
• Multi-Cell Changer
– 4, 6, or 8 Positions
– Manual, Automatic, Programmable
• Thermostatted Cell Holder
– Water-jacketted or Electronic Peltier system
• Batch Sampler - 40 Positions Carousel
• Specular & Integrating Sphere / Diffuse
Reflectance
Sample Handling Options
Multiple position CELL CHANGERS, MICROSIPPERS and Sequential Access BATCH SAMPLERS
Allow the Chemist complete
FLEXIBILITY for
MAXIMUM Sample
Throughput & Volume
on ANY of the BUCK/Cecil
UV-Vis Spectrophotometers
Software Features
A convenient INTERNAL Ink-Printer,
and several P-C Software Programs
provide many Data Processing and
Manipulation options for Qualitative &
Quantitative Work
Economical
UV-Scan and
sophisticated
GRAMS/UV &
Data-Stream
are available
Software Features
The INTERNAL Software built-in to the BUCK/Cecil
UV-Vis Spectrophotometers is capable of performing
General Analytical, Environmental, Biotechnology and
Clinical Assay WITHOUT the need for a Computer:
• Christian-Warburg & Protein / DNA Measurements
• Colorimetric Anion Calibrations stored in the instrument
• Wavelength Subtraction, Spectral Overlay & Smoothing,
Derivatization and auto Peak-Pick on all systems
• Multi-Component Analysis, with 1o / 2o and 3o Polynomial
Calibrations with up to 10 Standards with Statistics
APPLICATIONS
Primarily used for QUANTITATIVE
Analysis of Known Compounds, UVVis is one of the most popular
techniques in the Pharmaceutical,
Foods and Paints Industries, as well
as Water Laboratories
APPNOTE - Inorganic
SAMPLE: River Water for PHOSPHATE
PREPARATION: Digest w/ 10% HCl, add
NH4MoO4 & Hydrazine reagent, boil till
Blue color develops; measure at 810nm.
ANALYSIS: CE-2041 or VIS-100 w/
USEPA 2-pt Calibration
RESULTS: mg/L
or PPM in Sample
APPNOTE - Inorganic
DATE
3-10-00
3-24-00
4-08-00
4-22-00
5-10-00
Total-PO4
69 PPM
77 PPM
94 PPM
118 PPM
85 PPM
ortho-PO4
51 PPM
50 PPM
83 PPM
72 PPM
78 PPM
ANION assays by Colorimetry are the ACCEPTED
Standard World-Wide & much better (and less
expensive) than Ion-Chromatography
APPNOTE - Cosmetic
SAMPLE: Color-Dyes for Hair Shampoos
PREPARATION: Make 1:5 and 1:200
dilution of Dyes to compare to Samples
ANALYSIS: CE-2041 or -3041 Scanning
Spectrophotometer,
1cm. Glass Cuvette
RESULT: % Red /
Green / Blue based
on Color Standards
APPNOTE - Nutritional
SAMPLE: Cereals and Grains (preserved)
PREPARATION: Hydrolyze 2 grams w/ 5%
HCl, extract w/ Hexane for 1:10 dilution
ANALYSIS: CE-2041 or CE-3041 Scanning
Spectrophotometer, scan range 200-450nm. For
BHT, BHA and TBHQ Anti-Oxidants. Sample
solutions run in 10mm. Quartz Cells
RESULT: Parts-Per-Million TOTAL AntiOxidants based on USFDA Standard Procedure
APPNOTE - Nutritional
Sample
BHA
BHT
TBHQ
[Wavelength
410nm.
360nm.
285nm.]
Frosted Flakes35 PPM 27 PPM 11 PPM
Raisin Bran
16 PPM 41 PPM < 2 PPM
Rice Krispies
23 PPM 11 PPM 4.5 PPM
Scanning SPECTROPHOTOMETRY is an ideal
method to evaluate YOUR OWN products, or
material from the Competition
Other SPECTRO Analyzers from BUCK
BUCK Scientific also offers a series
of inexpensive, high-performing
and reliable Analytical
Spectroscopy equipment for special
Applications, Educational and
Dedicated Testing procedures
FLUORESCENCE SPECTROSCOPY (FLUOR)
Theory & Principle of OPERATION
FLUORESCENCE is a form of Molecular
“EMISSION” Spectroscopy; where a Sample in a
special Cuvette is exposed to invisible, highEnergy UV radiation, and certain parts of the
Organic Molecule will “give off” or EMIT lowerEnergy VISIBLE Light radiation.
Very specific & selective test for Drugs &
Vitamins, Alkaloids & Toxins
BUCK Filter Fluorometer
The advantage of
HIGH Sensitivity for
most compatible
materials makes
Fluorescence a
valuable analytical
tool for Forensic &
Drug applications
Intense BLUE
Fluorescence of
QUININE in a Drug
Formulation
BUCK Filter Fluorometer
The availability of a
wide assortment of
Excitation and Emission
Filters makes this
portable FL-380
instrument very
flexibility for a very low
cost… ideal for
Academic Teaching &
Industrial QA/QC work
Visible COLORIMETRY (COLOR)
Theory & Principle of OPERATION
This is for use in Academic Teaching classes
and for dedicated COLORIMETRIC
Analyses for materials such as ANIONS
(SO4, NO2, NO3, PO4, etc) and Visible
Colors of compound such as Pigments,
Chlorophyll and Dyes
Covers the range from 320 - 950nm.
BUCK Colorimeter
The VIS-100 is a
small, durable, selfcalibrating unit that
provides MEDIUM
resolution @ 8nm.
Bandpass, automatic
1-point Calibration
& Data Outputs for a
very low price