Transcript HAEMOGLOBIN ESTIMATION

HAEMOGLOBIN ESTIMATION
Sahli’s method
Aim
• To estimate the Haemoglobin levels in
mg/dl using Sahli’s method
Equipment
• Sahli’s haemoglobinometer with tube,
pipette, colour comparator and stirrer
• Hydrochloric acid, 0.1N
• Distilled water
• Blood sample
SAHLI’S HAEMOGLOBINOMETER
Haemoglobinometer tube
20 μl mark
Mark 2
Stirrer
Comparator
Haemoglobinometer pipette
PROCEDURE
 Fill N/10 HCl up to mark 2 of the tube
 Pipette out 20 μL of blood, wipe tip with cotton to
remove excess blood
 Introduce blood into HCl in the tube
 Mix, keep for 10 minutes
 Add distilled water, stir, compare colour in
comparator
 Continue adding water and mixing until colour
matches
 Read lower meniscus on tube for Hb in g/dL
Fill N/10 HCl up to mark 2 of the tube
Mark 2
N/10 HCl
Pipette out 20 μL of blood
20 μL mark
Wipe excess blood
around tip with cotton
Blood sample
Introduce blood into tube
N/10 HCl
Mix, keep for 10 minutes
Add distilled water, stir
Continue adding, stirring until colour
matches with standard
Read Hb from lower meniscus,
express as g/dl
Read Hb level
QUESTIONS
1. Describe the procedure
2. What is the principle behind the method?
3. What are the advantages & the disadvantages
of this method?
4. What other methods are available?
5. Which method is considered ideal?
6. What is the reference haemoglobin range?
7. What is the importance of haemoglobin
estimation?
MORE RELATED QUESTIONS
1. Define anemia / polycythemia
2. Classify anemia.
3. Name some causes for anemia /
polycythemia
4. What is Drabkin’s solution?
____________