Transcript Diagram of the Blood Clotting Cascade

Biomaterials Research - Manfred Maitz
The Blood Clotting Cascade
Manfred Maitz
FZ-Rossendorf
www.manfred.maitz-online.de
Biomaterials Research - Manfred Maitz
The Clotting Cascade
Thrombin
Fibrin
polymers
Fibrin
monomers
Fibrinogen
Biomaterials Research - Manfred Maitz
The Clotting Cascade
Intrinsic Pathway
Extrinsic Pathway
Surface Contact
Collagen
FXII activator
F XII
Tissue/Cell Defect
F XIIa
F XI
F IXa
Ca2+
F VII
F III (Tissue
Thromboplastin)
F VIIIa
Platelet Factor 3
Factor F X
Ca2+
Factor F Xa
F Va
Prothrombin I
F XIIIa
Crosslinked
Fibrin Meshwork
Ca2+
F XIa
Ca2+
F IX
F VIII
F VIIa
Ca2+
Ca2+
Factor F X
FV
Thrombin
F XIII
Fibrin
polymers
Fibrin
monomers
Fibrinogen
Biomaterials Research - Manfred Maitz
Serine Proteases
RN
O
RN
C
:O
O-
Ser
H
HN
C
O
HN
H
RC
RC
Ser
Tetraedric
Transition State
Break of the
Peptide Bonding
HN H
RC
RN
RN
H2O
C
:O
Release of the H
N-terminal peptide
O
OH
Ser
O
C
O
Enzyme-acetyltransition-state
Ser
Biomaterials Research - Manfred Maitz
The Fibrinolytic System
Clotting
Cascade
Fibrinogen
Fibrin
degradation products
Fibrin-clot
Plasminogen
T-PA
F XIIa
HMWK
Kallikrein
Urokinase
Streptokinase
Plasmin
Biomaterials Research - Manfred Maitz
Effect of Antithrombin III/Heparin
Intrinsic Pathway
Extrinsic Pathway
Surface Contact
Collagen
FXII activator
F XII
Tissue/Cell Defect
F XIIa
F XI
F IXa
Ca2+
F VII
F III (Tissue
Thromboplastin)
F VIIIa
Platelet Factor 3
Factor F X
Ca2+
Factor F Xa
F Va
Prothrombin I
F XIIIa
Crosslinked
Fibrin Meshwork
Ca2+
F XIa
Ca2+
F IX
F VIII
F VIIa
Ca2+
Ca2+
Factor F X
FV
Thrombin
F XIII
Fibrin
polymers
Fibrin
monomers
Fibrinogen
Biomaterials Research - Manfred Maitz
Thrombin Time (TT)
Intrinsic Pathway
Extrinsic Pathway
Surface Contact
Collagen
FXII activator
F XII
Tissue/Cell Defect
F XIIa
F XI
F IXa
Ca2+
F VII
F III (Tissue
Thromboplastin)
F VIIIa
Platelet Factor 3
Factor F X
Ca2+
Factor F Xa
F Va
Prothrombin I
F XIIIa
Crosslinked
Fibrin Meshwork
Ca2+
F XIa
Ca2+
F IX
F VIII
F VIIa
Ca2+
Ca2+
Factor F X
FV
Thrombin
F XIII
Fibrin
polymers
Fibrin
monomers
Fibrinogen
Biomaterials Research - Manfred Maitz
The Quick (PT)-Test
Intrinsic Pathway
Extrinsic Pathway
Surface Contact
Collagen
FXII activator
F XII
Tissue/Cell Defect
F XIIa
F XI
F IXa
Ca2+
F VII
F III (Tissue
Thromboplastin)
F VIIIa
Platelet Factor 3
Factor F X
Ca2+
Factor F Xa
F Va
Prothrombin I
F XIIIa
Crosslinked
Fibrin Meshwork
Ca2+
F XIa
Ca2+
F IX
F VIII
F VIIa
Ca2+
Ca2+
Factor F X
FV
Thrombin
F XIII
Fibrin
polymers
Fibrin
monomers
Fibrinogen
Biomaterials Research - Manfred Maitz
Partial Thromboplastin Time [(a)PTT]
Intrinsic Pathway
Extrinsic Pathway
Surface Contact
Collagen
FXII activator
F XII
Tissue/Cell Defect
F XIIa
F XI
F IXa
Ca2+
F VII
F III (Tissue
Thromboplastin)
F VIIIa
Platelet Factor 3
Factor F X
Ca2+
Factor F Xa
F Va
Prothrombin I
F XIIIa
Crosslinked
Fibrin Meshwork
Ca2+
F XIa
Ca2+
F IX
F VIII
F VIIa
Ca2+
Ca2+
Factor F X
FV
Thrombin
F XIII
Fibrin
polymers
Fibrin
monomers
Fibrinogen
Biomaterials Research - Manfred Maitz
Surface Sensitive Steps
Intrinsic Pathway
Extrinsic Pathway
Surface Contact
Collagen
FXII activator
F XII
Tissue/Cell Defect
F XIIa
F XI
F IXa
Ca2+
F VII
F III (Tissue
Thromboplastin)
F VIIIa
Platelet Factor 3
Factor F X
Ca2+
Factor F Xa
F Va
Prothrombin I
F XIIIa
Crosslinked
Fibrin Meshwork
Ca2+
F XIa
Ca2+
F IX
F VIII
F VIIa
Ca2+
Ca2+
Factor F X
FV
Thrombin
F XIII
Fibrin
polymers
Fibrin
monomers
Fibrinogen