Transcript Rinse & Chill Technology: Novel Intervention for Reduction
Rinse & Chill
Technology
Novel Intervention for reduction of undesirable bacteria on beef carcasses and On-going Protection from coliforms and E. coli in vacuum packaged ground beef
Rinse & Chill
Technology
• Developed by MPSC, Inc. – St. Paul, MN • Post bleed vascular technology • Involves vascular rinse and chill of carcasses with a solution of saccharides, sodium chloride and phosphates
Rinse & Chill
Technology
• Provides for statistically “cleaner” carcass samples with respect to
aerobic
when compared with controls microorganisms 2 h cooler sponge samples/3 sampling dates
APC Control R&C N
90
Ave.
CFU/cm2
1224
% Reduction P value (95% - .05)
90 731
40.3% .039*
Rinse & Chill
Technology
• Provides for statistically “cleaner” carcass samples with respect to
coliforms
microorganisms when compared with controls 2 h cooler sponge samples/3 sampling dates
Coliforms Control N
90
Ave.
CFU/cm2
97
% Reduction Frequency P value (95% - .05)
22/90
R&C
90 0.7
99.3%
11/90 0.125
Control vs. Rinse & Chill
10000 1000 CFU/cm2 100 10 1 0.1
C T C = Control T = R&C APC Coliforms
Rinse & Chill
Technology
• Provides for statistically “cleaner” carcass samples with respect to
aerobics
microorganisms when compared with controls 24 h cooler sponge samples/3 sampling dates
APC Control N
50
Ave.
CFU/cm2
580
% Reduction P value (95% - .05) R&C
50 341
41.2% .009*
Rinse & Chill
Technology
• Provides for statistically “cleaner” carcass samples with respect to
coliforms
microorganisms when compared with controls 24 h cooler sponge samples/3 sampling dates
Coliforms Control R&C N
50
Ave.
CFU/cm2
345
% Reduction Frequency P value (95% - .05)
41/50 50 111
67.8%
31/50
0.002*
Rinse & Chill
Technology
• Provides for statistically “cleaner” carcass samples with respect to
generic E. coli
microorganisms when compared with controls 24 h cooler sponge samples/3 sampling dates
Generic
E. coli
Control R&C N
50
Ave.
CFU/cm2
294
% Reduction Frequency
31/50
P value (95% - .05)
50 48
83.7%
16/50
0.0008*
1000 Control vs. Rinse & Chill
100 CFU/g 10 1 C R&C APC Coliforms G. E. coli
Rinse & Chill
Technology On-going Protection
• Significant on-going protection in further processing of carcasses, such as in ground beef • Inoculated with
E.
coli 0157:H7; • Vacuum packaged; 40F; 2 trials; triplicate Ave. (n=6)
C R&C P value (95% - .05)
Day 1
Log10 CFU/g
4.65
4.76
0.141
Day 17
Log10 CFU/g
3.81
Day 31
Log10 CFU/g
3.06
3.39
0.005*
2.05
0.007*
Day 50
Log10 CFU/g
2.68
Day 70
Log10 CFU/g
2.77
1.81
2.07
0.002* 0.0006*
Day 92
Log10 CFU/g
2.27
0.82
0.021*
5 4.5
4 Log10 CFU/g 3.5
3 2.5
2 1.5
1 0.5
0
Vacuum-packaged ground beef; 40 F
E. coli
0157:H7 Control vs. Rinse & Chill
C R&C 0 20 40 Day 60 80 100
Rinse & Chill
Technology Summary Carcass sponge Ground shoulder/chuck (50/50)
• Reduction in APC – 40% – 41.2% • Reduction in coliforms – 99.3% – 67.8% • Reduction in generic
E. coli
– 83.7% • Control –
2.38 log reduction
in
E.
coli 0157:H7 in 91 days • Rinse & Chill –
reduction
in
E.
3.94 log
coli 0157:H7 in 91 days