Transcript Health New Zealand Test Power Point Poster #1

Poster 5-11, Society for Research on Nicotine
and Tobacco (SRNT) Dublin, April 30, 2009
®
Ruyan
E-cigarette
Bench-top tests
Murray Laugesen QSO MBChB FNZCPHM
Health New Zealand Ltd, Christchurch NZ.
www.healthnz.co.nz
[email protected]
Background
Electronic cigarettes, without tobacco, flame or smoke, claim to be cigarette
substitutes and to deliver nicotine safely, without smoke toxicants. Are
these claims justified?
Aims
To assess the Ruyan® e-cigarette and its
mist for safety, emissions, and nicotine
dose.
Participating laboratories, materials, methods
Environmental Science and Research,
Porirua, NZ.
Hill Laboratories, NZ.
Hort Research, Hamilton, NZ.
Labstat International ULC, Canada.
Lincoln University, NZ.
National Radiation Laboratory of NZ.
Syft Technologies Ltd. Christchurch,
NZ
Duke University Center for Nicotine
and Smoking Cessation Research
(CNSCR) Bioanalytical Lab. NC USA:
British American Tobacco, Group R&D,
(Southampton, UK)
TEST MATERIALS
Ruyan in Beijing supplied V8
Classic e-cigarettes and 16 mg
nicotine-labeled cartridges exfactory to test laboratories, directly,
or via distributors. Most were
manufactured in 2008 and tested in
2008-9. Batteries were re-charged
before testing, and fresh cartridges
used. An ISO machine-smoked
cigarette of 1 mg labeled tar yield
provided smoke toxicants.1
PUFFING MACHINE SETTINGS
ISO mode was the default mode of
one 35 ml puff every 60 seconds, of
duration 2 seconds, no vent blocked.
Intense mode was 50 mL every 30
seconds, duration 2 seconds, no vent
blocked.
Manual puffs. A gas-tight system of
syringe with 3-way tap was used to
enumerate puffs (35 mL) per
cartridge.
Methods
Figure
MIST
Particle size distribution. BAT used a fast
ASSEMBLED E-CIGARETTE electrical mobility spectrometer (TSI 3090,
Radiation. NRL of NZ measured the
MN,USA) measured Ruyan V8 mist in ISO
assembled e-cigarette for Pb210
mode. The TSI was unable to measure
gamma- emitting nucleotides.
tobacco smoke, and so another fast electrical
Operating temperature. Using a 51-II
mobility spectrometer (Cambustion DMSHand-held theromocouple, the heating 500, UK) was used instead. Duke CNSCR
coil, the vaporising contact, and the
used the Marple Series 290 6-stage personal
exiting aerosol were measured every 30 cascade impactor for e-cigarette
minutes for five hours of use.1
mist.(Murugesan 2009 pers.comm).
Pressure drop. Open pressure drop was
measured (3 replicates) at a flow of 17.5 Mist - Selection of toxicants for testing
mL/second (based on ISO puff of 35
Over 60 toxicants were selected for testing,
mL over 2 seconds), and compared with based on published priority lists of cigarette
a cigarette.1
smoke toxicants:
Battery. Power, current and voltage were 9 toxicants recommended by WHO TobReg
measured using a Fluke 183 Digital
committee for mandatory lowering;9
Multimeter with two probe test leads,
36 smoke toxicants prioritised by
during actual puffing in ISO and intense toxicological risk assessment by Fowles &
mode on a single port Borgwaldt
Dybing, including the 9 above;10
R58.02) puffing machine.1
17 toxicants, additional to the above 45,
routinely tested in British Columbia,11
CARTRIDGE LIQUID
known loosely as the Hoffman analytes.
Propyelene glycol, glycol and ethanol
Analytical methods
were tested by using GC against
Carbon monoxide (CO): An NDIR analyser
internal standards.1,2
was calibrated against standard CO/N2
TSNAs (Tobacco-specific nitrosamines)
mixtures. 35 puffs were drawn in ISO mode
were analysed by LC-MS/MS at
from e-cigarette by single port smoking
Labstat.15
machine into a 3L Tedlar bag, then exhausted
Monoamine oxidase inhibition was
tested by kynuramine substrate against into analyser.1 Two replicates.
Other smoke toxicants. BAT and Labstat
a tobacco extract by ESR.3
used their own standard methods to extract,
PAHs 34 were tested by Hort Research
analyse and quantify each toxicant in mist
using GCMS.4,5
Heavy metals were measured by ICP-MS and in cigarette smoke. Using two replicates
and based on 50 e-cigarette machine puffs
at ESR. From cartridge liquid, ESR
the toxicant in the mist was estimated as the
extracted nicotine into methanol using
amount expeced in the same number of puffs
D3 nicotine as internal standard, and
as were required to machine smoke the
analysed by single ion GCMS, with
calibration based on nicotine bitartrate.6 tobacco cigarette control.
Nicotine: 50 puffs of 35 mL mist each were
The headspace above the liquid was
collected on a single Cambridge filter pad,1,2
scanned by HS-SPME and GCMS at
kept refrigerated until the nicotine was
Lincoln University,14 and analysed by
SIFT Ltd using SIFT-MS (selected ion extracted with NaOH and analysed by
GCMS. (Use of liquid impinger bottles
flow technology), with some difficulty
resulted in analyte loss.)1,2, 7
due to quantity of propylene glycol
______________________________________________________
present.16
6. Fitzmaurice P. Heavy metal testing of Ruyan cartridge liquid.
_____________________
1. Proctor C, Murphy J. Analysis of the Ruyan Classic ecigarette. British American Tobacco Group R&D. 15 April
2009.
2 Scientific Analysis of E-cigarettes by British American
Tobacco Research &Development. November 2007.
3. Lewis A. Investigation into the effect of RUYAN cartridge
exposure on Monoamine oxidase enzyme activity in vitro.
ESR October 2007.
4. Benzoalpha pyrene. Hort Research Report to ESR 19
November 2007.
5. Polycyclic aromatic hydrocarbons in Ruyan e-cigarettes.
Hort Research. Analysed 17 March 2008.
Inductively coupled plasma mass spectrometry (ICP-MS)
Environmental Science and Research (ESR) Porirua 2008.
7. Murugesan T. Cascade impactor test and other nicotine analyses
of E-cigarette mist. Duke University CNSCR Durham NC. 2009.
8. Fitzmaurice P. Testing of Ruyan e-cigarette cartridges for nicotine
content. Porirua ESR. 18 December 2007.
9 Burns DM, Dybing E, Gray N, Hecht S, et al. Mandated lowering
of toxicants in cigarette smoke: a description of the World Health
Organization TobReg Proposal. Tobacco Control 2008; 17:132-41.
10. Fowles J, Dybing E. Application of toxicological risk
assessment principles to the chemical constituents of cigarette
smoke. Tobacco Control 2003; 12: 424-430.
Results
THE ASSEMBLED E-CIGARETTE
Radiation. NRLNZ found no gamma-emitting
nucleotides.
Operating temperature, at the heating coil averaged
54˚C, at the point of vapourisation, 29.9˚C, and 23.1˚C in
the exiting aerosol.1
Pressure drop (PD) was greater for the e-cigarette ( 152
mm WG) than for an unlit conventional cigarette (80-120
mmWG). However in use, with either product, the
pressure drop could be 50 mm WG higher.1
Battery. The lithium-ion battery was rated by Ruyan to
last 1300 puffs – we tested it for 300 puffs only. Per puff
it delivered approximately 0.1 mW of power. However at
17 months, towards the end of a battery’s shelf life the
power delivered faded after 6 puffs. Intense puffing
consumed more power.1
THE CARTRIDGE LIQUID
The cartridge (labeled 16 mg), contained 1.06 g of liquid,
composed of 1.4% nicotine, (13 mg1 to 14 mg8 ) 90%
propylene glycol, and 0.1% glycerol, water 8.8%.
Tobacco specific nitrosamines totalled 8 ng per g.2. There
was no monoamine oxidase inhibitor activity, as can be
detected in tobacco smoke extract.3
Of 35 PAHs (polycyclic aromatic hydrocarbons) tested in
Ruyan® cartridges in 2008, the carcinogen, benzoalphapyrene was not detected.4. Of the remainder, four
carcinogens were detected, in cartridge liquid but these
were not human carcinogens, and have no human cancer
potency ratings.5 The carcinogenic heavy metals,
including As, Cd, Chr, Ni, Pb were not detected – at the
limit of 0.2 micrograms per gram.6
Other compounds preent in the liquid included toluene,
ethanol, bipyridine, propan-1-ol, bipyridine, tripropylene
glycol and beta nicotyrine found in trace quantities.2
Headspace above cartridge liquid
Acetaldehyde was detected at 5 ppm, and acrolein at 0.3
ppm.15 Other cigarette toxicants found to be absent or not
detected at 0.01 parts per million or more, were
acrylonitrile, benzene, 1,3-butadiene, m-o- and p-cresols,
ethylene oxide, HCN, styrene, and xylenes.16
_____________________________________________________________________
11. Government of British Columbia. Ministry of Health Services. Accessed April
2009. http://www.health.gov.bc.ca/tobacco/ttdr.html
12. Labstat International ULC. Analytical tests completed for BAT. In: Analysis of the
Ruyan Classic e-cigarette by British American Tobacco Group Research and
Development 15 April 2009.
13. Graves I. Report no. 468304. 60 ml sample of mist from 11 mg nicotine e-cigarette
cartridge. Thermal desorption tubes. Hill Laboratories. Hamilton New Zealand, 5
September 2008.
14. Sherlock R. Head Space Solid-Phase Micro-Extraction (HS-SPME) analysis of
headspace above e-cigarette cartridge liquid. Lincoln University, Soil and Physical
Sciences Group. www.lincoln.ac.nz
15. Rickert W. Determination of Tobacco specific Nitrosamines by LC-
MS/MS. Project NZ9. Nov.30, 2007. Labstat International ULC. Kingston
Ontario, Canada.
16 Langford V. SIFT-MS Headspace Analysis of Nicotine Cartridges from Ruyan eCigarettes. Christchurch. SYFT Ltd. February 2008.
17. Laugesen M. & Fowles J. Marlboro UltraSmooth: a potentially reduced exposure
cigarette. Tobacco Control 2006: 15: 430-435.)