Transcript Biodegradation of Petroleum Hydrocarbons in Marine and

Biodegradation of Petroleum
Hydrocarbons in Marine and
Freshwater Environments
RYAN JENNINGS
Introduction
 Purpose: to review the process and applications of
biodegradation
 Influencing factors (chemical, temperature,
nutrients)
 Methods of biodegradation
Chemical Composition
 Has the most significant effect on biodegradability
 Four ranks of susceptibility (linear
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alkanes>branched alkanes>small aromatics>cyclic
alkanes)
Linear – methane, propane
Branched – iso, neopentane
Aromatics - benzene
Cyclic - cyclopropane
Temperature
 Controls pollutant chemistry and bacterial growth
rate
 Can take place over wide range of temperatures
(correlates directly)
 Most effective in fresh water between 20-30°C
Nutrients
 Nitrogen, phosphorous, and iron play a vital role
 Especially important in freshwater
 Overabundance can hinder biodegradation rate
(Chaineau)
Methods - Bioaugmentation
 The introduction of cultured microorganisms into an
environment in which they are capable of
biodegrading and mineralizing contaminants.
 Used when the contaminated site lacks microbes
with the catabolic potential or to supplement the
indigenous microbes
 Enlarges gene pool for more genetic variation and
faster reproduction (increased biodegradation rate)
Methods - Biostimulation
 The addition of rate limiting nutrients such as
phosphorus, nitrogen, oxygen, and carbon
 Used when capable microbes are present, but lack
nutrients needed to sustain
 Both bacteria and nutrients introduced through
injection wells, slurry walls, or trenches
Conclusion
 Biodegradation of hydrocarbons is a complex process
that relies on the nature of the hydrocarbon, and the
environment
 Main problem with this method is the lack of
knowledge on proper bacterial strain use
References
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Cooper, C.K., Kvenvolden, K.A., “Natural seepage of crude oil into the marine environment” Geo-Marine Letters, vol. 23, no.3-4,
p. 140-146, 2003.
Mulkins Phillips, G. J., Stewart, J.E., “Distribution of hydrocarbon utilizing bacteria in Northwestern Atlantic waters and coastal
sediments,” Canadian Journal of Microbiology, vol. 20, no.7, p. 955-962, 1974.
Adebusoye, S. A., Ilori, M. O., Amund, O. O., Olatope, S. O., Teniola, O. D., “Microbial degradation of petroleum hydrocarbons in a
polluted tropical stream,” World Journal of Microbiology and Biotechnology, vol. 23, no. 8, p. 1149-1159, 2007.
Brusseau, M. L., “The impact of physical, chemical, and biological factors on biodegradation,” Proceedings of the International
Conference on Biotechnology for Soil Remediation: Scientific Bases and Practical Applications, p.81-98, 1998.
Cooney, J. J., “The fate of petroleum pollutants in fresh water ecosystems,” Petroleum Microbiology: New York, Macmillan, 1984
p. 399-434.
Atlas, R. M. “Effects of temperature and crude oil composition on petroleum biodegradation,” Journal of Applied Microbiology,
vol. 30, no. 3, p. 396-403, 1975.
Chaineau, C. H., Oudot, J., Rougeux, G., Yepremian, C., “Effects of nutrient concentration on the biodegradation of crude oil and
associated microbial populations in the soil,” Soil Biology and Biochemistry, vol. 37, no. 8, p. 1490-1497, 2005.
Fritsche, W., Hofrichter, M., “Aerobic degradation by microorganisms,” Environmental Processes-Soil Decontamination:
Weinheim, Germany, Wiley-VCH, p. 146-155, 2000.
Boon, N., Dejonghe, W., Seghers, D., Top, E. M., Verstraete, W., “Bioaugmentation of soils by increasing microbial richness:
missing links,” Environmental Microbiology, vol. 3, no. 10, p. 649-657., 2001.
Aldrett, S., Autenrieth, R. L., Bonner, J. S., Mills, M. A., Stephens, F. L., “Microbial degradation of crude oil in marine
environments tested in a flask experiment,” Water Research, vol. 31, no. 11, p. 2840-2848, 1997.
Margesin, R., Schinner, F., “Low temperature bioremediation of a waste water contaminated with anionic surfactants and fuel oil,”
Applied Microbiology and Biotechnology, vol. 49, no. 4, p. 482-486, 1998.
Kalogerakis, N., “Ex-situ Bioremediation of Contaminated Soil,” Soil and Sediment Remediation: London, UK, IWA Publishing, p.
151-175, 2005.