Transcript Methyl Group Loss

Kevin A. Negrete
- President
DNA Workshop
(Bologna, Italy)
To live better… longer
To increase the quality
of life after 40
90
80
70
60
50
40
30
20
10
0
Neanderthal
Paleolithic
(12000 BC)
Neolithic Stone Classic Roman Midevil England
Age (9500 BC) (500's - 1400's)
(1500's)
1800's West
1900's West
2000 West
Two main factors contributing longer (historical) lifespan
Public
Health
Nutrition
Two main factors contributing to limited lifespan
Environmental
(controllable)
Genetic
(non-controllable)
Cellular
Oxidation
Preprogrammed
Methyl Group
Loss
Oxidative Stress
(Free Radicals)
Preprogrammed
shortening of
Telomere length
reduce cellular oxidation
the formation of nascent oxygen (O2)
and the elimination of free radicals (O+)
Two main factors contributing to limited lifespan
Environmental
(controllable)
X
X
Genetic
(non-controllable)
?
?
Cellular
Oxidation
Preprogrammed
Methyl Group
Loss
Oxidative Stress
(Free Radicals)
Preprogrammed
shortening of
Telomere length
or is it?
Research has allowed scientists to identify central aging
system in cells – and specifically the aging clock in DNA
The two basic DNA markers that regulate aging are
Telomere length and Methyl Group Loss
Inside the center or nucleus of a cell, our genes are located on twisted,
double-stranded molecules of DNA called chromosomes.
Telomere
At the ends of the chromosomes are stretches of DNA called telomeres,
which protect our genetic data, make it possible for cells to divide.
Telomeres have been compared with the plastic tips on shoelaces
because they prevent chromosome ends from fraying and sticking to
each other, which would scramble an organism's genetic information to
cause cancer, other degenerative diseases, or death.
Each time a cell divides the telomeres get shorter (by 30-200 BP).
Healthy cells only divide between 50-70 times. When telomeres get too
short, the cells no longer can divide and becomes inactive, sustain
genetic damage and ultimately die.
Conception
Birth
Death
15,000 Base Pairs
10,000 Base pairs 1,500 Base Pairs
Similar to telomere shortening – the loss of Methyl Groups (CH3)
shows a specific preprogrammed degradation throughout
mammalian lifespan. The degradation is accelerated by oxidative
stress, poor nutrition, lack of exercise, and environmental toxins.
H
H
H
H
H
H
H
H
H
Final Common Pathway of Aging – DNA Demethylation
Birth
10% loss
25 yrs
20% loss
50 yrs
30% loss
75 yrs
40% loss
Death
Understand true anti-aging (DNA) mammalian
chemistry consists of 2 goals: elongate telomers
and increase DNA methylation.
ENHANCED
NUCLEOTIDE
SUPPLEMENATION
Longevity of Control vs. Treated Animals
2500
2000
1500
1500
1000
75
0
150
750
Control
750
Treated
Record mammalian longevity achieved
through weekly injection of DNA and RNA
• Study began with 750 day old rats with usual longevity of 800
to 900 days
• Identical conditions except treated animals received nucleic
acid injections
• Post 8 weeks control rats lost fur and vitality, whereas treated
animals increased muscle mass, libido, and activity levels
• Control animals all died in less than 150 days from start of
study
• Treated animals lived a minimum of 850 up to 1500 additional
days from start of study, doubling and even tripling the usual
life span
Understand true anti-aging (DNA) mammalian
chemistry consists of 2 goals: elongate telomers
and increase DNA methylation.
HOMOCYSTEINE
What is Homocysteine?
Homocysteine is a toxic amino acid by-product produced
by our own organism and accumulates in the bloodstream.
Elevated homocysteine is not only the indicator of, but is
the CAUSE of many major diseases includin Alzheimer’,
Arthritis, Cancer, Chronic Fatigue, Depression, Diabetes,
Heart Attacks, Infertility, Obesity, Strokes, Thyroid
Problems, and Ulcers
Homocysteine also accelerates the oxidation of LDL
Homocysteine Level VS Cardiac Risk Ratio
Research has shown that higher
HOMOCYSTEINE levels are directly
related to demethylation and telomere shortening
3 ways to detoxify HOMOCYSTEINE
Methionine
ATP
S-adenosyl-methionine (SAMe)
DMG
Betaine
(TMG)
B12
Folic Acid
Choline
Homocysteine
(Hcy)
Zn
Methyl Group Transfer
Metabolic Pathways
Vitamin B6
Cysteine
Glutathione
Donates CH3 to:
S-adenosylhomocystei DNA
Proteins
ne (SAH)
Lipids
Carbohydrates
Myelin
Detox Pathways
Neurotransmitter
Production
And others
We have developed THE ENGINE OF REMETHYLATION
making cells younger through
Enhanced Nucleotide Supplementation
DNA
RNA
Purines
Pyrimidines
Adenine
Cytosine
Guanine
Thymine
Adenine
Cytosine
Guanine
Uracil
Methionine
ATP
S-adenosyl-methionine (SAMe)
DMG
Betaine
(TMG)
B12
Folic Acid
Choline
Homocysteine
(Hcy)
Zn
Methyl Group Transfer
Metabolic Pathways
Vitamin B6
Cysteine
Glutathione
Donates CH3 to:
S-adenosylhomocystei DNA
Proteins
ne (SAH)
Lipids
Carbohydrates
Myelin
Detox Pathways
Neurotransmitter
Production
And others
Could life-extension and anti-aging be as simple as
supplying the body with DNA and RNA?
DNA
RNA
Purines
Pyrimidines
Adenine
Cytosine
Guanine
Thymine
Adenine
Cytosine
Guanine
Uracil
Primary source of nucleotides is endogenous synthesis
from amino acids and simple precursors
• Therefore nucleic acids have generally been
considered nonessential nutrients
• Under stress, however, some tissues unable to
produce enough nucleotides to support tissue needs
• In these conditions, nucleic acids bases may become
essential nutrients for optimum tissue repair and
function
• Glycine, Serine, Aspartic Acid, Glutamine
Nucleic Acids : Food & Supplementation
So why doesn’t
everyone just take a
giant nucleic acid pill?
Orally ingested nucleic acids undergo intensive
metabolic degradation in upper GI tract
• Over 99% of purine bases oxidized to uric acid before
entering portal circulation
• About 95% of pyrimidines are metabolized before
leaving intestinal lining
• Only 3% of ingested pyrimidines reach liver
Photo
Acoustic
Resonance
Laser
Laser Enhancement Technology
Nutrient molecules get distorted
during food or supplementation
processing. Result: reduced
nutrient assimilation by the cells.
Laser Enhancement Technology
reshapes nutrient molecules.
Result: increased nutrient
assimilation by the cells.
Control Betaine HCl
Laser Homogenized
Betaine HCl Crystal
Extensive experimental and clinical evidence for
benefit from nucleotide supplementation
• Increased survival in mice with Staph aureus
bacteremia from 21% to 71%
• Significantly increased survival in mice with
Candida sepsis – greater effect with parenteral
nucleotides than oral administration
• Survival in animals with high dose radiation
exposure increased from 5% to 50%
• Single injection of RNA in mice post tumor
immunization improved outcome from 0% to 40%
long term survival
• Rate of liver regeneration in rats post 70%
hepatectomy significantly greater if IV nucleic acid
bases given
• In young rats with chronic diarrhea, appearance and
enzyme function of intestine greatly improved
• Improved growth and maturation in young animals
• Infants given formula supplemented with nucleotides
to mimic content of breast milk showed more
benevolent intestinal flora an less diarrhea (50 times
more nucleic acids in breast milk vs. formula which is
significant in developing countries)
• Supplemented infants had better lipid profiles with
higher HDL cholesterol
• Significant improvement in human cellular immune
function
• Significant improvement in memory in animals and
humans
• Accelerated wound healing
ATP delivery specifically associated
with numerous benefits
•
•
•
•
Improved pulmonary function, even in cystic fibrosis
Enhanced cardiac function – strengthening the heart
Relief of nerve pain and improved nerve conduction
Direct antitumor effects (RNA nucleotide) specifically
incubation with ATP breast and prostate cancer
• Synergistic antitumor effects in conjunction with
chemotherapy – in vitro studies suggest up to 90%
dose reduction possible for certain cell types
• IV ATP has halted progression of tumor cachexia
(advanced cancer with rapid weight loss)
50 mcg/kg/minute x 24 hours x 3 weeks
• Protection of tissues from radiation injury
• Increased natural killer cell function
• Survival of patients in ICU for shock (multi-organ
failure) increased from 70% to 100%
Current research
Enhanced
Nucleotide
Supplementation
Reducing Homocysteine reduces the
risk of death from the top 5 killer diseases:
Heart Attack
Strokes
Cancer
Diabetes
Alzheimer's
by 80%
by 82%
by 33%
by ??%
by 50%
Homocysteine Levels (units = micromoles/litre)
Below 6.3 = Very Low Risk
6.4 - 9.0 = Low Risk
9.1 - 15.0 = High Risk
+ 15.0 = Very High Risk
Every 5 point decrease in homocysteine level =
50% reduced risk of cardiovascular death
26% reduced risk of cancer death
Methylation Formula Study Dose Response Curve
Homocysteine Level- Treatment Group (n=22)
11
10
9.2
9
8
7.1
7
6.8
P=.00001
6.1
P=.0000
1
6
0
1
2
P=.00001
3
Active Supplementation Group Months 0-3
Methylation Formula Study Dose Response Curve
Homocysteine Level- Placebo Group (n=11)
9
7.9
8
7.8
P=
NS
7.3
7
7.9
P= NS
P= NS
6
0
1
2
3
Methylation Formula Study Dose Response Curve
14
Homocysteine Level- Treatment Group for subjects with Baseline Homocysteine Level > 10 (n=7)
13.2
12
10
9.3
8.3
P=.009
8
P=.001
7.3
P=.00001
0
1
2
3
Active Supplementation Group Months 0-3
Population study completed in Western Norway with over
18,000 subjects – Hordaland Study
Directly established a relation between homocysteine
levels and chronological aging
Homocysteine level of >13 was associated with someone
65 years old
Homocysteine level of <7 was associated with someone
25 years old
Bryan Maphalala - AIDS Victim
CD4
Count
Viral
Load
CD4 vs.
T-Cells
Normal
600/ul
0/ml
30%-60%
AIDS
200/ul
50,000/ml
10%
Brian
10/ul
201,000/ml
1.17%
Bryan Maphalala - AIDS Victim
Before taking CELLFOOD and
CELLFOOD DNA-RNA CD4 Count: 10
units; Viral Load: 201,000
After taking CELLFOOD & CELLFOOD DNA-RNA
for 6 months Mrs. Winnie Mandela congratulated
him for his work with HIV and AIDS people
Does it really work?