Transcript 2007-04-03 ABRF BIC Oral

Bioinformatics Committee
Exploration of the sPRG2006
Study Data Sets
ABRF2007, Tampa, FL
Bioinformatics Committee (BIC)
General Goals
● Examine sPRG2006 datasets
● Act as the foundation for an ABRF "iPRG" – Proteome Informatics
Research Group
Members
● Jeffrey A. Kowalak - National Institutes of Health
● William S. Lane - Harvard University
● Alexey Nesvizhskii - University of Michigan
● Brian C. Searle - Proteome Software
● Sean L. Seymour - Applied Biosystems (chair)
● David L. Tabb - Vanderbilt University
2
Specific Questions of Interest
● What is the best answer set of proteins for the sample?
– Which 'bonus proteins' reported by respondents last year are
correct?
– Careful protein inference assumed to be key in answering this.
– A 'best answer' for the sample would allow these data sets to be
used for future informatics tools development, testing, etc.
● Can experts in informatics get consistent results using
different analysis tools?
● How similar are existing protein inference tools?
● Can we dissect acquisition and informatics sources of
variation in the quality of answers from last year’s sPRG
study?
3
Analyzed Data: sPRG2006 Study
● 78 labs responded to last year's sPRG Study
● 24 labs provided raw data (used Tranche repository)
● Converted data to mzData, mzXML, .dta, and .mgf
● 19 sets were ultimately examined
● These sets included:
– Data from all major vendors' instruments
– ESI and MALDI, 1D-LC, 2D-LC, and gel workflows.
● Only MS/MS data were considered.
4
A Diversity of Analysis Tools were Used
Peptide
Identification
Protein
Inference
Decoy DB
Assessment
Kowalak
Mascot
MassSieve
Run but not
Manual
applied as filter
Lane
SEQUEST
Proteomics
Browser Suite
spectral level,
<1% FDR
Nesvizhskii
SEQUEST
Protein
Prophet
Run but not
Protein prob>0.95
applied as filter
Searle
Mascot
Scaffold
Run but not
Protein prob>0.95,
applied as filter 2 pept. prob>0.95
Seymour
Paragon
Pro Group
protein level,
<5% FDR
Pro Group
conf>99%
Tabb
MyriMatch
IDPicker
spectral level,
<5% FDR
Required 3 distinct
peptides
5
Final Validation
Required 3 distinct
peptides
Method to Finding Consensus
● Stage 1: Free exploration of data sets
● Stage 2: From inclusive to focused: BIC Candidates FASTA database
– Included all accessions that anyone suggested might be correct:
○ Original respondents’ protein lists.
○ BIC from UniProt, IPI, NCBI, Sigma recombinant proteins, and a contaminant database.
– Searched concatenated decoy database.
● Stage 3: Final searches on BIC FASTA DB
– Upon seeing rough results, we removed unnecessary non-human proteins.
All Swiss-Prot human proteins
Strong IPI, NCBI candidates
Strong non-human candidates
Strong Sigma sequence candidates
Total
6
15,637
4
39
1
15,681
(31,362 including reversed)
BIC Consensus Protein Annotation
● Not everyone searched all 19 files – total of 84 searches
● Of 247 total proteins that had any evidence:
– 133 were vouched for by at least one person
– Reduced to 104 by requiring a majority in same lab
7
BIC Consensus Protein Annotation
● Total proteins detected in any lab’s data
Expected: Intended 49 proteins
49
Expected: Additional human proteins
20
Expected: E.coli
Total desirable protein detections (originally in sample)
Expected: Digestion enzyme
Variable: Human keratins
3
72
2
12
Variable: Sheep proteins (from wool)
5
Variable: Trypanosome proteins
9
Variable: E.coli proteins
4
Total other detections (avoidable artifacts)
8
104
30
Final Searches: See the BIC Poster for All
Search Results
Cell gives peptide metric
reported for protein
Ambiguity among
Accession Numbers
Each row is one putative protein detection
9
Block for each
participant lab
Column per analysis
by one BIC member
Final Searches: See the BIC Poster for All
Search Results
participant
proteins
BIC 1
BIC 2
BIC 2
BIC 2
BIC 2
BIC 2
lab 28629
Trypanosome
protein
contaminants
10
BIC Assessment of Data Set Quality and
Consistency of Analysis
● Not everyone counts peptides the same way.
● However, trends were clearly consistent.
● To assess consistency of our analyses and the
fundamental quality of the acquired data sets, peptide
measures were normalized.
11
Normalization of Peptide Metrics across
BIC Member Analyses
● Considered 4
data sets.
● Sum each
protein per BIC.
● Compute the
mean value for
each protein
across BIC.
● Determine a
normalization
factor for each
BIC member.
Expected Proteins 1-49
12
Consistency of BIC on Individual Data Sets
● For cross-validation, consider data sets not used to determined
the normalization factors.
13
Expected Proteins 1-49
Consistency of BIC on Individual Data Sets
● Averaging all BIC measures for any single data set yields
a consensus on the information content or quality of the
data set.
With error bars indicating
variance across BIC
Expected Proteins 1-49
…or for simplicity, just
the trend line.
Expected Proteins 1-49
14
Expected Proteins 1-49
BIC Consensus: Qualities of Acquired Data
15
Expected Proteins 1-49
BIC Consensus: Qualities of Acquired Data
16
Proteins 50-80
BIC Consensus: Qualities of Acquired Data
E.coli
17
Proteins 100-145
Re-grading of sPRG2006 Results
● Using the annotation, the picture changes slightly.
18
Conclusions
● A master protein list has been produced for the sPRG2006 study sample.
● Despite using very different tools, experts were able to get surprisingly
consistent results.
● A key to recognizing this consistency was careful alignment of reported
proteins preserving ambiguity among accession numbers where possible.
● By doing our own informatics analyses, we were able to order the participants’
data sets by our assessment of the quality of the acquired data.
● We reconfirm the conclusion of last year’s sPRG study that the expertise of
the user seems to be the dominant factor in the quality of the acquired data.
– There are both good and bad sets from high res and low res instruments, as well as
newer and older instruments.
● Re-grading submitted protein lists with our master protein list allowed
confirmation of additional correct proteins reported by last year’s respondents.
● To what extent is expertise a factor in consistency of informatics analyses?
● www.abrf.org/sprg
19
Acknowledgements
● BIC Members
–
–
–
–
–
–
Jeffrey A. Kowalak - National Institutes of Health
William S. Lane - Harvard University
Alexey Nesvizhskii - University of Michigan
Brian C. Searle - Proteome Software
Sean L. Seymour - Applied Biosystems (chair)
David L. Tabb - Vanderbilt University
● The sPRG
● Jayson Falkner (Phil Andrews lab, UMichigan)
● All sPRG2006 Study respondents who submitted your
raw data!
20
Appendix
21
Normalization of Peptide Metrics across
BIC Member Analyses
● Comparison of
the individual
means to the
group mean
allows the
derivation of a
correction
factor from a
simple fit to
y=mx.
22
sPRG2006 Proteomics Standard
www.abrf.org/sprg
23
Accsn
Entry
Protein
Accsn
Entry
Protein
P01008
ANT3_HUMAN
Antithrombin-III precursor
P01343
IGF1A_HUMAN
Insulin-like growth factor IA
P02768
ALBU_HUMAN
Serum albumin
P12081
SYHC_HUMAN
Histidyl-tRNA synthetase
P08758
ANXA5_HUMAN
Annexin A5
P02788
TRFL_HUMAN
Lactotransferrin
P55957
BID_HUMAN
BH3 interacting domain death agonist
P41159
LEP_HUMAN
Leptin
P00167
CYB5_HUMAN
Cytochrome b5
P61626
LYSC_HUMAN
Lysozyme C
P99999
CYC_HUMAN
Cytochrome c
P00709
LALBA_HUMAN
Alpha-lactalbumin
P04040
CATA_HUMAN
Catalase
P61769
B2MG_HUMAN
Beta-2-microglobulin
P62937
PPIA_HUMAN
Peptidyl-prolyl cis-trans isomerase A
P02144
MYG_HUMAN
Myoglobin
P02741
CRP_HUMAN
C-reactive protein
Q15843
NEDD8_HUMAN
Neddylin
P00915
CAH1_HUMAN
Carbonic anhydrase 1
P01127
PDGFB_HUMAN
Platelet-derived growth factor B-chn
P08311
CATG_HUMAN
Cathepsin G
Q06830
PRDX1_HUMAN
Peroxiredoxin 1
P01031
CO5_HUMAN
Complement C5
P16083
NQO2_HUMAN
NRH dehydrogenase [quinone] 2
P00918
CAH2_HUMAN
Carbonic anhydrase 2
P02753
RETBP_HUMAN
Retinol-binding protein
P07339
CATD_HUMAN
Cathepsin D
P01112
RASH_HUMAN
GTPase HRas
P06732
KCRM_HUMAN
Creatine kinase M-type
P63165
SUMO1_HUMAN
Small ubiquitin-related modifier 1
P15559
NQO1_HUMAN
Quinone reductase 1
P00441
SODC_HUMAN
Superoxide dismutase [Cu-Zn]
P01133
EGF_HUMAN
Pro-epidermal growth factor
P02787
TRFE_HUMAN
Serotransferrin
P05413
FABPH_HUMAN
Fatty acid-binding protein
P01375
TNFA_HUMAN
Tumor necrosis factor
P06396
GELS_HUMAN
Gelsolin
P10599
THIO_HUMAN
Thioredoxin
P08263
GSTA1_HUMAN
Glutathione S-transferase A1
P10636
TAU_HUMAN
Microtubule-associated protein tau
P09211
GSTP1_HUMAN
Glutathione S-transferase P
P62988
UBIQ_HUMAN
Ubiquitin
P69905
HBA_HUMAN
Hemoglobin alpha chain
P51965
UB2E1_HUMAN
Ubiquitin-conjugating enz E2 E1
P68871
HBB_HUMAN
Hemoglobin beta chain
P63279
UBC9_HUMAN
Ubiquitin carrier protein 9
P10145
IL8_HUMAN
Interleukin-8
O00762
UBE2C_HUMAN
Ubiquitin-conjugating enzyme E2 C
P01344
IGF2_HUMAN
Insulin-like growth factor II
Note: UBC9_HUMAN - formerly UBE2I_HUMAN
SYHC_HUMAN - formerly SYH_HUMAN
sPRG2006 Protein Identification
Total
# of
Unique Labs
sPRG2006 Proteins:
Unique Peptides:
Median Unique:
Mean Unique
Incorrect (Only lab):
sPRG50 ALBU_HUMAN
CATA_HUMAN
ANT3_HUMAN
GELS_HUMAN
TRFE_HUMAN
SYH_HUMAN
TAU_HUMAN
KCRM_HUMAN
GSTP1_HUMAN
CYB5_HUMAN
TRFL_HUMAN
PRDX1_HUMAN
LEP_HUMAN
MYG_HUMAN
CAH2_HUMAN
CAH1_HUMAN
UBE2C_HUMAN
HBB_HUMAN
UBIQ_HUMAN
BID_HUMAN
HBA_HUMAN
CATD_HUMAN
CATG_HUMAN
PPIA_HUMAN
RASH_HUMAN
LYSC_HUMAN
GSTA1_HUMAN
SODC_HUMAN
NEDD8_HUMAN
ANXA5_HUMAN
CO5_HUMAN
PDGFB_HUMAN
UBE2I_HUMAN
THIO_HUMAN
NQO2_HUMAN
CRP_HUMAN
SUMO1_HUMAN
NQO1_HUMAN
B2MG_HUMAN
IGF1A_HUMAN
RETBP_HUMAN
IL8_HUMAN
IGF2_HUMAN
EGF_HUMAN
CYC_HUMAN
FABPH_HUMAN
LALBA_HUMAN
TNFA_HUMAN
UB2E1_HUMAN
24
P02768
P04040
P01008
P06396
P02787
P12081
P10636
P06732
P09211
P00167
P02788
Q06830
P41159
P02144
P00918
P00915
O00762
P68871
P62988
P55957
P69905
P07339
P08311
P62937
P01112
P61626
P08263
P00441
Q15843
P08758
P01031
P01127
P63279
P10599
P16083
P02741
P63165
P15559
P61769
P01343
P02753
P10145
P01344
P01133
P99999
P05413
P00709
P01375
P51965
2102
1153
1100
1190
1898
1066
1059
1224
426
322
1252
549
353
439
712
554
428
425
493
383
288
521
452
378
422
255
368
247
181
438
159
259
241
169
208
147
133
184
113
106
122
85
85
97
79
107
40
33
40
76
74
74
73
71
70
70
68
68
68
67
67
66
65
64
64
64
64
63
63
63
62
62
61
60
58
57
57
57
55
52
50
49
49
47
42
42
39
39
35
34
34
33
30
26
24
19
15
14
>5
23258
4788620
47631v
53017v
70788
29105
21013
10266v
71489
14474
38501
58696
F4755
01903
28629
10812
31960
53178
213479
43691
74187
20014
13895
32344
39563
39440
150866
91919
32181
R0001R
R0002R
R0003R
R0004R
R0005R
Accession
77708v
29504v
72079
65215
98166
58941
29115
27960v
17017
42457
12874
46013
0715
26081
92616
22455
21079
10085
37266
97330v
53908
77526
74643
62562
27406
00144
22069
98506
40003
25519
06511
14997
1062000
11641
51958
54601
91741
72791
96751
05013
69089
26402
18984
00700
Source Entry
3-5
8165883487
1-2
Legend:
48 48 47 46 46 46 46 45 45 45 45 44 44 44 44 43 43 43 43 42 42 41 41 40 40 40 38 37 37 37 37 36 36 36 36 36 35 35 34 34 33 32 32 32 31 30 30 30 30 29 29 27 27 26 26 25 24 24 24 24 23 21 19 19 18 17 16 15 9 6 5 4 1 0 49 47 49 48 48
808 497 883 646 576 568 562 712 702 547 442 520 477 349 419 631 347 222 306 261 340 185 381 462 219 570 195 160 118 177 338 190
36
221 198 290 328 410 224 235 109
80
253 100 337 156 158 122 371 111 213 222 383 128
95
283 317 191
70
162 311
77
137 179
18
156 193 117 101
13
5 23
1
0
381 408 317 390 645
12 6 13 9 9 10 9 11 12 10 7 9 8 6 6 10 5 4 5 5 6 3 6 8 5 8 4 3 2 4 7 4 1 4 4 5 8 8 5 5 2 1 7 3 6 4 4 4 7 3 6 7 8 4 2 8 5 6 2 4 12 3 6 9 1 9 11 7 12 2 1 6 1 0 6 6 4 6 8
17 10 19 14 13 12 12 16 16 12 10 12 11 8 10 15 8 5 7 6 8 5 9 12 5 14 5 4 3 5 9 5 1 6 6 8 9 12 7 7 3 3 8 3 11 5 5 4 12 4 7 8 14 5 4 11 13 8 3 7 14 4 7 9 1 9 12 8 11 2 1 6 1 0 8 9 6 8 13
0 0 5 0 0 0 8 0 0 0 0 0 0 1 6 1 2 2 3 2 4 0 2 5 6 12 1 0 3 7 8 0 0 1 2 17 0 17 0 6 3 0 2 4 3 0 3 6 9 0 4 0 8 4 22 0 2 7 11 25 1 3 0 1 20 7 1 3 4 1 4 17 11 32 0 0 0 0 0
55
41
32
42
64
44
28
35
14
8
46
21
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17
22
14
17
14
10
11
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16
15
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18
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6
21
10
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7
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6
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53
16
21
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46
22
22
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6
51
11
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7
20
12
3
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8
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9
10
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4
6
6
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8
3
3
8
4
2
4
4
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70
41
39
40
75
54
29
40
16
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23
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22
23
18
17
13
10
13
9
23
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19
10
28
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6
26
4
11
12
7
14
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15
1
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1
55
26
27
36
56
22
29
25
9
6
45
13
7
12
19
13
9
8
10
8
6
14
14
14
14
10
12
9
5
7
8
7
10
8
9
5
6
7
8
50
28
22
25
45
33
28
33
7
6
23
16
8
19
16
11
11
12
3
7
7
13
15
14
12
10
16
6
2
12
3
9
9
6
2
4
6
5
2
9
6
3
8
6 3
11 4
5
1
3 2 6
5
3
2
49
27
12
34
46
13
38
25
9
4
37
13
8
14
12
12
14
10
6
13
6
13
11
11
12
7
13
10
6
5
4
10
2
8
9
2
4
6
5
5
4
4
4
6
3
46
26
25
39
45
31
27
30
8
7
27
13
8
10
16
13
13
11
7
6
10
11
13
9
11
6
12
8
6
9
5
2
8
4
5
3
3
9
4
5
3
2
1
5
2
2
2 2
1
2
1
67
38
31
34
51
30
24
49
24
11
28
14
7
14
35
23
12
16
19
12
6
21
15
17
11
14
9
5
4
11
6
6
6
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5
4
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60
25
35
31
37
24
35
33
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10
41
18
16
8
32
24
11
15
24
14
14
12
14
11
12
5
5
4
2
10
5
13
11
7
13
6
3
9
4
9
2
6
42
30
29
28
47
33
18
23
10
6
15
16
7
14
15
12
10
10
9
13
10
11
11
7
12
5
20
4
9
10
6
5
8
3
1
4
3
5
5
5
3
3
3
2
3 9 1
1 5 5
8
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6
3
48
23
21
26
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21
18
17
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6
23
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7
6
14
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39
26
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56
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32
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11
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43
17
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36
16
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7
8
6
9
9
9
10
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4
14
6
7
7
2
3
4
3
3
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5
4
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4
2
4
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1
42
23
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18
41
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6
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12
8
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69
26
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50
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36
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51
15
11
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25
18
15
25
5
4
22
6
6
9
11
12
6
1
10
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7
7
6
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1
6
3
5
4
6
4
5
7
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4
2
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3
7
3
2
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2
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1 4
4
1
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2 2 4
5 2
1
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6 2
2
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3
9 2
1
14 1
4 2
2
1
3 6 2 2
1
1
3
3
2
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26
12
12
10
18
11
11
11
2
5
11
4
4
4
6
5
7
3
4
6
3
3
6
2
4
2
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1
3
1
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4
5
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20
21
21
17
22
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14
13
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13
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5
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12
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6
8
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6
7
7
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26
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9
13
23
13
11
13
3
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5
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8
8
6
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6
1
7
5
4
6
3
5
5
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2
3
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2
3
3
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5
4
1 3
1 2
1 3 2
3
1
1 1 1
2 2 2
1
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1
1
4
1
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31
15
12
14
33
19
9
21
6
6
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6
10
8
8
6
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5
4
10
9
5
6
3
4
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28
10
5
7
20
3
7
8
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4
12
5
3
3
9
6
3
4
3
5
2
1
2
2
4
2
1
1
1
2
2
39
20
21
17
30
12
22
16
6
6
21
9
7
10
19
12
11
6
7
10
8
5
5
9
2
36
23
23
28
38
26
20
24
10
8
29
13
3
9
10
10
8
9
4
5
10
9
8
7
13
5 7
3
4 9
2 4
5 5
3 6
14
3
12
8
9
5
18
3
2
8
9
5
3
4
4
6
5
5
9
3
5
4
5
9
5
5
7
2
4
4
4
3
3
4
4
2
40
25
27
23
46
27
22
23
10
4
30
14
5
7
10
12
7
12
90
7
6
13
7
12
12
4
7
3
16
3
6
8
1 7 7
4
1 3 5
5
1 6
3
2 4 8
7
4 4
3 3 4
3
1
2
2 1 4
1
1 1 2
1 1
1 5 3 5
6 7
1
2 4
3
17
10
10
6
16
9
6
13
3
4
9
4
3
2
7
6
20
9
10
5
8
5
6
11
1
4
9
3
2
3
5
5
6
4
3
5
2
4
2
2
1
2
2
10
6
5
6
6
8
4
8
2
2
4
4
3
4
5
3
2
2
1
4
1
2
4
2
4
1
3
14
12
4
3
12
7
6
6
3
4
10
6
4
4
8
41
21
17
14
30
18
15
6
4
14
6
8
10
4 9
8 7
2
6 11
4 8
3 12
5 8
3 10
4
5
7
16 5
2
6
1 3
2 8
3 4
1 6
2 4
4
1 2
3
3
1 4
2 2
4
4
3
3
6
5
7
5
2
5
2
1 2 1
3
2
1
2 3 1
4 2 2
4 1
1
2
3 1 1
3
1
4
1
1
2 1
1
3 1
1
1
1
2
1
1 2
15
12
13
12
13
5
12
12
4
4
11
3
6
3
6
4
3
4
5
8
3
3
3
4
1
4
1
6
2
1
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
14
18
16
14
9
9
12
11
6
3
10
5
4
4
13
7
8
6
3
8
4
2
4
6
2
1
4
1
1
2
15
8
9
15
27
6
11
15
2
5
1
3
6
4
3
3
8
5
6
3
4
4
20
14
18
25
27
7
18
22
10
5
9
11
7
8
12
8
10
8
6
10
4
12
4
7
7
2
7
4
4
8
3
4
2
4 7 8
1 2 1
2 5
3
7 4
1 2
1 3
1 2
1 3
1 2 2 3
1 3 1
1
2
3
2
1 2 3
3
1
13
23
20
20
26
14
16
19
5
4
2
13
5
5
9
5
5
8
4
5
2
11
2
5
6
4
6
3
39
21
26
22
35
27
18
20
6
5
27
10
5
24
19
10
7
24
7
10
14
5
7
11
6
4
10
8
3
4
7
3
4
10
12
10
12
10
10
3
7
3
4
3
5
5
2
4
2
36
11
10
1
19
2
13
17
6
2
27
4
8
5
8
9
5
8
12
6
1
2
2
2
5
2
1
1
3
6 4
4 3
11 1
11
1
3 3
12
8
6
5
4
7
4
8
1
1
5
3
3
2
2
1
3
2
2
2
4
2
1
1
2
6
1
2
1
6 1 1 3
3
2
5
7
1
5
1 1 2
1
7
5
4
3
11
1
2
6
2
21
16
10
14
28
12
7
16
7
5 16
1 10
1
4
5
1 10
1
4 7
3 7
1 1
2 3
8
2 7
1 9
2 6
1 4
1 3
1 2
5
1 2
1 6
8
4
4
6
8
1
5
8
2
1
6
4
1
3
6
5
4
2
1
3
1
3
3
2
1
1
1
1
1
1
1 1 1
46
21
20
8
34
17
34
8
7
10
10
15
6
4
17
12 3
5 3
24
4
14 4
10 3
5
12 2
3 1
3
10 4
6 2
5
10 7
2 2
5
3
4 4
6
3 3
7 5
5 2
4
2 5
2
3
2
25
3
5
7
32
3
5
11
2
2
8
6
4
1
9
2
5
2
4
4
8
7
5
5
11
4
6
11
4
4
10
2
2
5
4
2
2
2
2
1 2
3 4
3
4 3
1 2
4 3
1
1
1
1 1
4
48
14
19
18
11
5
6
3
4
4
4
8
2
2
7
4
3
2
5
4
6
35
19
4
3
48
8
5
10
13
14
5
4 8
5
4 3
3
4 4
2
6 2
3
11
2
2
6 1
24
9
11
9
19
12
12
16
10
10
13
11
26
18
11
24
1
7
2
5
3
14
3
7
63
6
16
22
23
32
21
7
6
6
14
40
6
10
3
4
3
9
11
24
6
13
3
2
4 3 7
6
17
3
6
5
5 7
6 11
2 6
3 1 8
1
5 10
2 1 8
2
7
12
7
1
13
8
4
1 2 1 5
5
5
8
5
8
9
20
5
7
10
4
3
5
6
2
2
4
4
5
2
1
1
12
7
6
5
4
5
4
6
2
15
2
1
4
1
1
1
7
3
4
1
1
4 3
1
4
1
1
1 4
25
14
15
9
26
13
13
19
4
2
5
6
10
1
6
7
2 22
12 11 19
3 11 7
10 18
11 11 15
8 11
2 14
3 11
11 16
2 6 6
4
7
15
4 4 5
3 5
6 8 9
1 6
10
4 4
5
5
3 4
2 6 9
11
4
2
3 2 1
5
1
1
1
1
1
1
1
7 20 24
7 7 17
3 6 18
8 20
4 15 27
2 3 14
7 11 15
21
2 1 11
5 7
4 8 14
2
12
1 5
2 9
1
9
1
9
1 3 9
3 7
5 47 8
4
2 1
12
2 1 11
1 8 10 12
1 10 10 11
1 13 13 8
12 11 8
1 10 26 7
1 16 18
1
11
1 12 24 7
9
1 5 7 5
6
1 12 5
7
1
14 7
6
7 5
1 8
8
5
16
1 7
5
4 4
1
7
1 7 11
4 4 1
1
1
1
1
2
10
4
6
12 6
8
1
1
1
15
13
20
4
17
1
6
9
2
1
1
3 2
3
9
5
1
1
1
1 10 12
1 4
2
1
11
6
1
3
4
2
1 4 2
1
34
28
20
22
48
36
35
22 24
7
5 1
14
6 3
4 2
10
7 5
7
8 1
8 5
2
6
4
13 3
6
2
6
3 1 6 1
9
4 1 9 1
1
5
2
9 2
4 2 11
7 2
1 2 5
1
4
2
1
2
3
2 10 13 2
19
1
1 4
1
4
3
7
2 2 3
2
4
3
13
15
19
23
39
21
26
13
12
14
27
29
8
14
6
3
26
8
7
7
11
7
9
8
5
6
6
14
7
6
11
5
3
5
2
11
1
4
3
5
6
5
2
6
1
4
7
2
1
2
2
7
1
2
4
28
21
15
21
26
16
13
19
9
4
35
7
5
6
15
20
5
8
7
8
3
12
13
5
6
2
8
1
2
12
2
3
5
3
6
3
4
8
1
3
2
1
4
5
2
1
3
27
11
12
15
25
7
21
9
6
4
27
5
4
3
14
4
5
7
4
6
1
12
10
4
4
4
3
3
4
7
1
6
4
5
4
3
3
2
2
2
3
1
3
1
1
4
1
1
2
26
16
15
15
29
19
14
14
6
3
31
7
3
6
14
4
6
7
7
3
5
11
12
7
8
11
8
2
3
11
4
8
5
3
4
4
5
4
2
2
4
4
3
1
2
6
53
48
32
36
40
36
24
24
7
8
51
10
7
12
15
13
12
11
7
6
5
12
16
7
10
4
7
6
4
21
3
6
6
8
10
8
7
15
3
2
6
2
2
6
9
2
2 2
4 4
Proteins Identified by 2 or More Labs
1-2
Legend:
RG Observed
A1AG1_HUMAN
hu
P02763
RG Observed
TETN_HUMAN
hu
P05452
RG Observed
SBP1_HUMAN
hu
Q13228
RG Observed
HBD_HUMAN
hu
P02042
Epith
K2C1_HUMAN P04264
IGF1B_HUMAN P05019
Epith
K1C10_HUMAN P13645
Epith
K1C9_HUMAN P35527
RG Observed
GST26_SCHJA
exogenous P08515
Epith
DESP_HUMAN P15924
RG Observed
KAIN_HUMAN
hu
P29622
CO8G_HUMAN P07360
GSTA2_HUMAN P09210
RG Observed
K22E_HUMAN
hu
P35908
RG Observed
MUC_HUMAN
hu
P01871
Ig
IGHA1_HUMAN P01876
RASN_HUMAN P01111
RG Observed
CAP7_HUMAN
hu
P20160
Epith
HORN_HUMAN Q86YZ3
PRDX2_HUMAN P32119
RASK_HUMAN P01116
RG Observed
KAC_HUMAN
hu
P01834
SYHH_HUMAN P49590
Epith
DCD_HUMAN
P81605
VPS11_HUMAN Q9H270
HSPB1_HUMAN P04792
Q92468_HUMAN Q92468
S100P_HUMAN P25815
Epith
Q8N175_HUMAN Q8N175
FETA_HUMAN P02771
CO3_HUMAN
P01024
TYRO3_HUMAN Q06418
RASH_MSVNS P23175
Epith
PLAK_HUMAN P14923
Epith
K2C6A_HUMAN P02538
UBE2I_MESAU O09181
Q566M9_HUMANQ566M9
S10A9_HUMAN P06702
APOH_HUMAN P02749
SPTN5_HUMAN Q9NRC6
Q567Q0_HUMANQ567Q0
RG Observed
BLVRB_HUMAN
hu
P30043
THIO_PONPY
Q5R9M3
PHF7_HUMAN Q9BWX1
GSTA3_HUMAN Q16772
Q14015_HUMAN Q14015
LEG8_HUMAN O00214
GSTA5_HUMAN Q7RTV2
CALL5_HUMAN Q9NZT1
APOA1_HUMAN P02647
A1AG2_HUMAN P19652
PADI1_HUMAN Q9ULC6
NGF_HUMAN
P01138
KPYM_HUMAN P14618
KCRB_HUMAN P12277
Ig
HS90A_HUMAN P07900
CO4_HUMAN
P01028
25
92
84
97
62
149
39
87
86
73
54
24
16
15
33
20
14
11
4
16
12
11
9
8
7
6
6
4
3
41
26
24
21
16
15
12
10
9
8
7
6
6
6
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
31
29
20
15
11
10
9
8
7
7
6
6
6
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
6 6 8 8
4 1 3 4 6 3 2
1 5
1
3 2 12
2
2 5
4 6 3 1
2
6
14
2
2 11
3 4
10
8
7
3
1
8
10
2
7
5
4
8
2
2
1 3 1 2
6
1 4 1 5
5 3 8
1 1
11
3
22
3
1
1 1
1
13
2
1
5
2
1
2
1
2
6
10
2
1
12 3
5
1
2 7 7
1 11
47
4
10
27
29
1
7
1
10
1
1
4
2
2
2 13 2
1
12
2
6
4
1
2
6
1
1
1
6
6
2
4
2
6
2
4
8
3
3
2
2
3
3
1
5
1
1
2
3
2
1
3
2
1
1
1
1
1
22
19
20
6
6
18
2
19
8
11
8
4
7
5
7
3
8
5
1
3
6
1
3
3
5
1
4
2
3
2
1
4
3
1
3
1
1
2
2
1
1
2
2
2
1
1
1
1
1
1
1
1
1
1
1 1
3
1 6 1
5 3 1 2 13
2
11
2
2
1
5
2
2
1
5
1
5
4
9
6
1
2
3
7
4
14
4
3
5
2
1
5
8 13
14
16
>5
1
2 6
6
17 11
6 7
14 6
14 6
8 3
3
3
2
2 1
1
1
1
9
15
11
4
1 1
3-5
1
1
1
1
Final Searches
● Not all 19 data sets were searched by all 6 members of the BIC:
File search by x
BIC members
Number of Data Sets
Searched by this #
3
5
4
6
5
3
6
5
Total searches: 84
26