Transcript OLS v6 - Main Page — HT Sequencing

Technical Differences Between Kit Versions
Illumina GA
Single-end
v1.2
Illumina GA
Paired-end (Custom)
v1.5
Illumina GA
Paired-end
(Whole Human Exome)
AB SOLiD
Fragment Library
DNA Prep Kit
Illumina Single-End
Illumina Paired-End
Illumina Paired-End
AB SOLiD
gDNA input amount
3 ug
3 ug
3 ug
3 ug
Covaris target size
200 bp
200 bp
150 bp
100-110 bp
Selected size
gel-electrophoresis
200-300 bp
gel-electrophoresis
300 bp 2mm
AMPure DNA Purification kit
(SPRI beads) - NO GELS!
gel-electrophoresis
150-200 bp
Adaptor for making DNA library
In illumina kit
FC-102-1001
In illumina kit
PE-102-1001
In iIllumina kit
PE-102-1001
SureSelect AB Adaptor Set,
Agilent p/n G3361A,B,C
In illumina kit
FC-102-1001
1uL (out of 30uL)
14 cycles
In illumina kit
PE-102-1001
23uL (out of 30uL)
6 cycles
PE Oligo Only Kit
PE-102-1003
92uL (out of 90-95uL)
6 cycles
SureSelect AB Adaptor Set,
Agilent p/n G3361A,B,C
4 x 125 uL
12 cycles
500 ng
500 ng
500 ng
500 ng
PCR primer for amplifying DNA
library post-capture
Single-End specific
2uL (out of 15uL)
14 cycles
In SureSelect Kit (SureSelect
GA Primers)
Paired-End specific
14uL (out of 15uL)
12 cycles
In SureSelect Kit (SureSelect
GA Primers)
Paired-End specific
15uL (out of 15uL)
10-12 cycles
In SureSelect Kit (SureSelect GA
Primers)
DNA Polymerase for post-capture
amp
Herculase II Fusion DNA
Polymerase
Herculase II Fusion DNA
Polymerase
Herculase II Fusion DNA
Polymerase
AB Specific
15uL (out of 15uL)
12 cycles
In SureSelect Kit (SOLiD PostCapture Primers)
Platinum PCR SuperMix High
Fidelity
Invitrogen p/n 12532-016
Quantify DNA
NanoDrop
BioA DNA1000
High Sensitivity DNA
BioA DNA1000
High Sensitivity DNA
PCR primer for pre-amp DNA
library
Pre-capture Amp
prepped library amount for
SureSelect capture
Blockers
Post-capture Amp
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BioA DNA1000
or Qubit Fluorometer, qPCR
Agilent SureSelect™ Platform
Enabling Products for the Next-Generation
Sequencing Workflow