Solid-state Fermentation

by using DFM(Direct-fed Microorganism)

for Feed & Feed-additives

Application of Solid-state Fermentation Foods: Functional-foods and Food-ingredients Livestock: Probiotics Agricultural Area: Bio-fertilizer Pharmaceuticals: Functional Bio-active Ingredients

Microbial Fermentation

Method Liquid-state Fermentation: Alcohol Production Solid-state Fermentation: Fermented Food Controlling Parameters Chemical Factors: Nutrients, Growth Factors, etc Physical Factors: Temp., pH, Oxygen, etc Biological Factors:

Solid-state Fermentation

Advantage Less contamination by low water content Oxygen-supply, CO 2 -removal and temp-control by aeration Disadvantage Difficulty in Mixing: Low in homogeneousness Difficulty in temp-control by low conductivity Difficulty in On-line monitoring Experience-dependent

DFM(Probiotics) in Livestock

Direct-fed Microorganism Probiotics Definition: Microbial cell preparations or components of microbial cells that have a beneficial effect on the health and well being of the host.

Design of Solid-state Fermentor

Exhaust Line & Evaporation Door for Raw Materials-Supply & Inspection Liquid-supply Spray Nozzle Paddle-type Mixing Device Recovery for Final Products Aeration Holes: Temp-controlled Air-Supply through chamber Forced air-supply by Blower With Temp-controlled Air

Overall Fermentation Processes

Preparatory Stage Main Fermentation Manufacturing Stage Raw Materials: Feedstuff or Fermentation media DFM(Probiotics) Water Content Functioanl Ingredients

Control

Temp, Aeration & Mixing Microbial Growth Water Content

Two-stage Fermentation

1 st 2 nd Stage: Aerobic Process Stage: Anaerobic Process

Control:

Water content Temp & Mixing

TMF Packaging

Solid-state Fermentation Process

Preparatory Stage Media as Raw Materials: Feedstuff DMF(Probiotics): High-density Microbial Cells Functional Ingredients: Growth factors 1 st Stage: Aerobic Fermentation Microbial Growth (Proliferation) & Enzyme Production Providing Aerobic Condition: Air supply through aeration holes at the bottom of fermentor Continuous agitation (mixing) for aeration and mixing 2 nd Stage: Anaerobic Fermentation Stabilization of grown-microorganisms Producing Bio-active Microbial Metabolites & Flavours Simultaneous providing anaerobic condition: Air supply through top of fermentor according to temp-control Continuous agitation (mixing) for aeration and mixing

DFM(Probiotics) for Fermentation

Effective Microorganisms used for Solid-state Fermentation Lactobacillus species Bacillus species Aspergillus species Yeast ( Saccharomyces ) species

TMF (Total Mixed Fermentation)

TMR (Total Mixed Ration) Feedstuffs for Dairy Cow Simple Mixing, None-Fermentation TMF: Advanced TMR Fermented Feedstuffs Fermented DMF(Probiotics) Composition: Live Probiotics: Effective Live-microorganisms Microbial components: Bio-active Microbial Products & Metabolites Microbial Enzymes: Various Enzymes

Function of TMF in Livestock

TMF In Vivo Normalization of Microflora Protection of inflammation and diarrhea Prevention of various diseases Enhancement in immunity and feed efficiency Minimizing undigested materials Providing various bio-active compounds Minimizing fiscal smell and water content Effective for Waste-treatment Providing Well-being Environment Reduction in insect plague In Vitro

Equipments for DFM(Probiotics)

Refrigerator for Stock and media

Clean bench

For aseptic work

Shaking Incubator

for seed culture

Lab-scale & Pilot-scale Fermentor

Incubator for microbial growth

Equipments for TMF

Supply-Device

For Raw Materials Screw Conveyor Dumping

Liquid-supply Devices

For DFM & Liquids FRP Tank & Feeding Pump

Solid-state Fermentor Transfer Devices

For TMF(Product) Screw Conveyor

Packing Device

For Manufacturing Packing & Wraping

Solid-state Fermentor (I)

Aeration Unit Ring blower with Line heater Dual way for aeration: Top & Bottom of Fermentor Controlled by PLC (Temp & Process) Agitation (mixing) Unit Paddle-type Impeller Mixing & Holding Controlled by PLC (Temp, Aeration & Process) Others Liquid-supply Unit: Special Screw Unit: Pushing-out final product

Solid-state Fermentor (II)

Control Unit PLC control with Programmed Software Interlocked with Aeration-Temp-Agitation-Process Time Data-storage and Ethernet Communication User-friendly Interface Consultation Support by Highly Experienced & Educated Expertise Experiences in Manufacturing & Process Control Consultation in Design, Process & Manufacturing, Products A/S & On-site Support