Transcript The Right Solution Agilent 1200 Infinity

The Right Solution:
The Agilent 1200 Infinity
Series for LC Analysis
Laura Phillips
Application Scientist
Agilent Technologies
June 27th, 2013
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The Agilent 1200 Infinity Series
Where Do We Come From?
1100 Series
1200 Series
(1995 – 2006)
(2006 – 2010)
1100 Bin/Quat
HPLC
Bin (HPM)
Quat (LPM)
400 bar
5 mL/min
1200 RRLC
UHPLC
Bin (HPM)
600 bar
5 mL/min
Price: 1200 Bin+20%
1200 Bin/Quat
HPLC
Bin (HPM)
Quat (LPM)
400 bar
5 mL/min
1200 Infinity Series
1290 Infinity (2009)
UHPLC
Bin (HPM)
Quat (LPM - 2012)
1200 bar
5 mL/min
1260 Infinity Bin/Quat
UHPLC
Bin (HPM) – 5mL/min
Quat (LPM) – 10mL/min
600 bar
Price ~ 1200 Bin/Quat
1220 Infinity
UHPLC
Bin (LPM)
600 bar, 5 mL/min
Price < 1200 Quat
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The Agilent 1200 Infinity Series
Raising the Standard in HPLC – UHPLC for EVERY Lab
new
new
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June 27, 2013
The Agilent 1200 Infinity Series
Raising the Standard in HPLC – UHPLC for EVERY Lab
Method
Developement
Solution
Multi-method
Solution
High-Dynamic
Range DAD
2D-LC Solution
Online-SPE
Valve solutions
Bioinert
solutions
SFC solutions
GPC solutions
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1220 Infinity LC
A solution to all your needs
•
Dual gradient pump with integrated
degasser, 600 bar, up to 10 mL/ min, for
flexible and reliable analysis
•
Vial sampler with injector programming for
higher automation
•
80°C oven for one 25 cm column for better
results
•
Variable wavelength or diode array
detector with 80 Hz sampling rate for high
sensitivity
•
Easy: Small footprint, low height. One
cable.
Affordable with ChemStation/ EZChrom
VL software
Expandable: Any other module can be
added
•
•
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600 bar for HPLC and UHPLC
Same as with the 1260 Infinity LCs, existing conventional HPLC methods
from 1100/1200 Series LC can be run on the 1220 Infinity LC and giving the
same results as well as UHPLC methods on 3.0mm and 4.6mmID columns
can be run. Both with excellent performance!
Saving half of the time by transferring a HPLC
method to UHPLC, without loss of resolution.
Excellent retention and area RSDs even under
UHPLC conditions.
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Expandability
External tray for walk-up:
Together with Agilent‘s EasyAccess software the 1220 Infinity LC with external
tray is a perfect solution for walk-up environments!
Additional detectors:
Almost all 1260/1290 Infinity detectors and SQ-MS can be added!
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Innovation: 1220 Infinity Mobile LC Solution
Affordable, Flexible, Compatible – Everywhere!
Instrument Control Framework
• Boost Performance
Complete UHPLC system for
higher resolution and shorter
run times
• Save Time
Bring your laboratory to the
samples
• Reduce Costs
Most affordable system
giving you access to UHPLC
technology
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1260 Infinity LC
1260/1290 Valve Solutions
1260 Infinity Bio-Inert LC – Buffer Advisor
1260 Infinity SFC
1260 Infinity GPC
1260/1290 ELSD
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1200 Infinity Series valve solutions
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The largest and most flexible valve portfolio
Workflow- and Application-Automation Flexibility
Applications
Types
600 bar, 1200bar
Std, Bio-inert, µ-flow, prep
NEW Quick-Change Valve heads
for universal Valve Drive
2pos/4port duo
2pos/ 6port
2pos/10port
4pos/10port
6pos/14port
8pos/ 9port
12pos/13port
6pos/ 7port
2pos/ 8port
2pos/12port
Custom Valves
1290 Infinity TCC
1290 Infinity Flexible Cube
• Multi-method/Method Dev
• Column switching (2,4,6,8)
• Solvent selection (13 – 26)
• Matrix-stripping
• Sample Enrichment
• Online-SPE
• Detector selection
• Heart Cutting 2DLC
• Comprehensive 2DLC
• Alternating Column Regen.
• Process Control/ Sample
Sampling/ Fractionation
• Column Connection
1290 Infinity Valve Drive
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July 7, 2015
Automated Column Regeneration
2-position / 10-port valve head
• 1x 1290 Infinity
Thermostatted column
compartment (or 1290 Infinity
Valve Drive)
• 1x 2-pos/10-port valve head
• While the analysis is running
on column 1, column 2 is
regenerated using the flow
from a second pump
• After the analysis on column
1, the flow switches to
column 2 for analysis while
column 1 is regenerated.
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Sample Enrichment
2-position / 6-port valve head
• 1x 1290 Infinity
Thermostatted column
compartment (or 1290 Infinity
Valve Drive)
• 1x 2-pos/6-port valve head
• The analyte is retained and
concentrated on an
enrichment column while the
sample matrix flows to
waste.
• For analysis, the
concentrated sample is
flushed out of the
enrichment column to the
analytical column.
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Sample Cleanup
2-position / 6-port valve head
• 1x 1290 Infinity
Thermostatted column
compartment (or 1290 Infinity
Valve Drive)
• 1x 2-pos/6-port valve head
• Matrix components are
retained on a pre-column
while the analytes pass
through to the analytical
column for separation.
• After sample cleanup or
matrix stripping, the
precolumn is flushed in
reversed flow direction by
the second pump.
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Column Selection for Method Development
6-position / 14-port valve head
• 1x 1290 Infinity
Thermostatted column
compartment
• 1x 6-pos/14-port valve
head
• Up to 6 columns (4 columns
recommended because of
available space)
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Solvent Selection for Method Development
12-position selection valve head
Typically used with 1290 Infinity Valve Drive.
For Method Development systems:
1290 Infinity Valve Drive and new 12-position
selection valve head is fully supported
as pump-valve cluster and by Agilent
ChemStation Method Scouting Wizard
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1260 Infinity Bio-Inert LC
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Innovation: 1260 Infinity Bio-inert LC
Truly Bio-inert UHPLC System
1260 Infinity Bio-inert LC
• Boost Performance
Highly versatile (U)HPLC
system for small- and largemolecule analysis
• Save Time
Stainless steel system
Fast switching between bioand non-bio applications,
including cleaning
• Reduce Costs
Only one system to maintain
for bio- and non-bio analysis
1260 Infinity Bio-inert LC
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New Capillary Design Ensures Bio-inertness
Capillaries
Metal clad PEEK capillary
Newest fitting design: Hybrid technique
New capillary technolgy enables
600 bar
AND is completely metal free !!
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Buffer Advisor Software
Facilitates Workflows in Ion Exchange Chromatography
Manual preparation:
1. Tedious
2. Error-prone
3. Irreproducible (operator)
Buffer Advisor helps to:
1. Define stock solutions for salt and pH
gradients
2. Check if the buffer system is suitable
for your separation task
3. Create a corrected time table for a
salt- or pH gradient.
Part of Bio-inert pump,
G5611A
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Buffer Advisor User Interface
Area 1: Select Buffer and
Gradient Type
Area 2: Enter Gradient
Time Table
Area 3: Gradient Display
Section: Process
Gradient and Review
Optimized Gradient with
Compensation Steps
Area 4: Review
Composition of Stock
Solutions and Adjust
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1260 Infinity SFC
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Innovation: Hybrid SFC/UHPLC System
Double your Discovery
Hybrid SFC/UHPLC System
• Boost Performance
SFC with LC-like sensitivity,
unique 600 bar x 5 mL/min
power range
• Save Time
Efficient instrument utilization
and orthogonality through
unique switching capability
between LC and SFC
• Reduce Costs
Lower solvent expenses, less
waste, and only one system
to maintain. Upgrade from LC
to SFC
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Agilent 1260 Infinity Analytical SFC/MS System
1260 Infinity SFC and the G6100 SQ Mass Spectrometer
Analysis of leachables in
food packaging material by
SFC/MS
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1260 Infinity GPC
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High Performance GPC/SEC for Routine Work
High Performance GPC/SEC Solution
• Boost Performance
High performance pump & oven
for reproducible molecular
weights
• Save Time
RID warm up less than 1 hour,
“I have always been impressed by the
quality, robustness, reliability and easeof-use of their products, which have
served me well.”
- John P. O’Donnell,
Research Scientist, FUJIFILM Imaging Colorants
Ltd., Manchester, UK
complete system based on
industry-leading Agilent LC
systems for maximum uptime
• Reduce Costs
RID with built-in solvent recycle,
minimum training effort.
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1260 Infinity GPC/SEC Multi Detector Suite
Summary
Key Benefits
Comprehensive
Allows complex multi detector GPC
experiments to be performed routinely.
Versatile
The MDS can be controlled by software
or the keypad for maximum ease of
use, and can be used with any existing
modular or integrated HPLC system.
Convenient
Data from an existing detector can be
collected and processed through the
MDS.
High Quality
Results
The detector cells are heated for
optimum baseline stability and
minimum instrument downtime.
Adaptable
Can be expanded at any time to
include additional detectors.
Safe
Incorporates multiple solvent leak
detectors to prevent spillage of
potentially hazardous solvents.
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Agilent 1260 Infinity ELSD
& 1290 Infinity ELSD
Evaporative Light Scattering
Detection (ELSD)
Page 28
Introduction: ELSD General Benefits
• Universal detection
• More uniform response
• Gradient Compatible
• Complimentary to LC-MS
• Eliminates derivatization steps in analytical methods
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Agilent ELSD Portfolio
G4260B
G4261B
1290 Infinity ELSD
(Cooling deleted)
1260 Infinity ELSD
Light Source
Blue LED (480nm)
Temperature
range
Nebulizer: 25-90°C
Evaporator: ambient – 120°C
•
Features
•
Hydrocortisone limit of detection
30ng
RS232 Communication only
Blue Laser (405nm)
Nebulizer: 25-90°C
Evaporator: ambient – 120°C
•
•
•
•
•
•
•
•
•
1290 Infinity ELSD
(Standard, cooled)
Nebulizer: 25-90°C
Evaporator: 10°C – 80°C
Hydrocortisone limit of detection 4ng
5-9 x improvement in sensitivity over current model
Unique sub-ambient operation (greater response for volatile
compounds)
RS232 and LAN Communication
Voltage selection (110/240V) no longer required
Variable gas flow for uniformity of response
Fast data collection (up to 80Hz)
Controlled via ChemStation / EZChrom
Front Panel control of all parameters (Temperature, gas flow, data rate etc)
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Increased sensitivity, reduced baseline noise
The increased laser intensity
coupled with a high gain
photomultiplier and digital signal
processing increases signal and
reduces noise.
Signal/noise calculated using OpenLab Chemstation v1.04
Injection: 10µL (Hydrocortisone 50ng-on column)
Column: Zorbax Eclipse XDB-C18 (5µm, 4.6x150mm)
Mobile Phase: Water/ACN (80/20)
Flow Rate: 1.0 m/min
ELSD: Neb 40°C, Evap 60°C, Gas 1.05SLM
G4261B
S/N 158
G4260B
S/N = 5
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Triglycerides in Natural Oils
Sample: 2mg/ml Starflower Oil
Column: C18 5µm, 250x4.6mm
Eluent A: ACN
Eluent B: DCM
Gradient: 30-50% B in 40 mins; 50-90% in 2
mins, hold for 3mins
Flow Rate: 1.0ml/min
Inj Vol:
20µl
Detector: neb=40°C, evap=70°C, gas=1.4 SLM
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Environmental Application:
Characterizing Biodiesel
Column:
BDS Hypersil Cyano 3µm, 250x4.6 mm
Eluent A:
0.4% Acetic acid in Hexane
Eluent B:
0.4% Acetic acid in Methyl tert-Butyl Ether (MTBE)
Gradient: 100% A, hold 5 mins; 0-80% B in 10 mins,
hold 2 mins; 80-0%B in 3 mins
Flow Rate: 1.0 ml/min
Inj Vol:
20µl
Detector:
neb=40°C, evap=80°C, gas=0.8SLM
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1290 Infinity LC
1290 Infinity Quaternary Pump
1290 2D LC
1290 Infinity with ISET
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1290 Infinity Quaternary LC
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Innovation: 1290 Infinity Quaternary Pump
Quaternary Pump with Binary Pump-like Performance
1290 Infinity Quaternary Pump
• Boost Performance
Highest, binary-like accuracy
and precision for composition
and flow, with exceptionally low
delay volume
• Save Time
Accelerates method transfer
from R+D to QA and from
HPLC to UHPLC
• Reduce Costs
One pump for any applications:
Most universal – at HPLC-like
costs of ownership
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Agilent 1290 Infinity Quaternary Pump
Specifications & Benefits
Power Range
 1200bar up to 2mL/min
 800bar at 5mL/min
 For any kind of analysis
Flow Accuracy and Precision
< 0.07 % RSD or 0.01 min SD
± 1.0 % or 10 µL
 High RT precision in
isocratic runs
Composition Range
1-99 %
Composition Accuracy and Precision
 Wide analytical range
< 0.15 % RSD or 0.02 min SD
± 0.4 % (1-99 % Composition B)
 High RT precision in
gradient runs
Delay Volume
< 350 µL
 For fast quaternary gradients
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1290 Infinity 2D-LC Solution
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Innovation: 1290 Infinity 2D-LC Solution
Square your Separation
1290 Infinity 2D-LC Solution
• Boost Performance
New levels of separation power,
detection sensitivity and
precision in 2D-LC
• Save Time
A single ultra high peak
capacity run instead of many
conventional runs – with 1min
method setup!
• Reduce Costs
Special features to reduce
solvent and valve cost, upgrade
paths for existing LC-systems
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Data Analysis
For heart-cutting 2D-LC by Agilent‘s OpenLAB CDS ChemStation ed.
For comprehensive 2D-LC Agilent recommends:
GC Image LCxLC Edition Software from GC Image LLC.
Key features offered:
• Direct Agilent datafile import
• Peak identification, integration, annotation
• Comparative Analysis and Visualization
• Compound-libraries
• MS-data handling
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Hardware
2. Dimension
Module-flexibility
One or two
1290 Infinity
TCC
1. Dimension
1290 Infinity
Binary Pump
1260 Infinity
Capillary Pump
1260 Infinity Binary
or Quaternary Pump
1290 Infinity
Binary Pump
1290 Infinity
Autosampler or 1260
HiP Autosampler
1260 Infinity
Autosampler
Optional
1260/1290
Infinity
Detector
Optional
1260/1290
Infinity
Detector
For 1st
dimension
chromatogram
and peaktriggering
1260/1290
Infinity
detector
To monitor
waste-line
Almost any Agilent pump or autosampler in the 1st dimension!
Almost any detectors are supported!
A 1290 Infinity Binary Pump for the 2nd dimension is required.
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Applications examples
- components of beer samples
Analysis of two different commercially available beer samples with different
separation modes
1st dimension - Reverse Phase (C18) 2nd dimension - Reverse Phase (Phenyl)
Beer B
Separation by RP (Phenyl)
Beer A
Separation by RP (C18)
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1290 Infinity Binary LC w. ISET
Page 43
Innovation: ISET
Intelligent System Emulation Technology
Intelligent System Emulation Techn.
• Boost Performance
The same retention times
and peak resolution as the
target system
• Save Time
No method redevelopment or
system modification required
• Reduce Costs
Minimized investment and
service costs, with one
system for all methods
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What is Method Transfer?
Different Aspects
From particle size to particle size (HPLC to UHPLC)
 often associated with a change of instruments due to constraints in max. pressure, extracolumn band broadening etc.. Some fine-tuning of method might be required due to
frictional heating effects
 available tools : Agilent Method translator, Third Party Method development SW (ACD
Labs, ChromSword etc.)
From one eluent type or phase chemistry to an other
 Method development
 available tools: Method development SW (Chromsword, ACD Labs, etc.)
From column dimension to column dimension (e.g. 4.6 to 2.1 mm i.d., 50 mm to 100 mm)
 recalculate flow rates, recalculate gradient times, adjust connection capillaries and flow
cells, due to delay volume results may vary, method might need revalidation
 available tools: Agilent Method translator
From Instrument to Instrument
 with method/instrument change: Isocratic Hold / Pre-injection, Vol. Modification
 available tools w/o method or instrument change : none until ISET
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Publication number 5990-9546EN
Publication number 5990-9703EN
Publication number 5990-9703EN
Publication number 5990-9545EN
Publication number 5990-9703EN
Intelligent System Emulation Technology
Agilent Technical and Application Notes
Page 46
Instrument Control Framework
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June 27, 2013
Innovation: Instrument Control Framework
Integrate your Agilent LC with other CDS
Instrument Control Framework
• Boost Performance
Full functionality of all Agilent
LC Systems in non-Agilent
data systems
• Save Time
Fast plug-and-play updates
to latest Agilent LC features,
modules, and systems
• Reduce Costs
Access new Agilent features
in non-Agilent software,
without extra coding
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June 27, 2013