Transcript Slide 1

The D3 Technologies advantage
D3 Technologies – SERS products / solutions / services
SERS technology:
delivering rapid trace level detection for
Pharmaceutical Cleaning Verification
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The Company
Technology
Klarite ™
Applications
© 2008 D3 Technologies Ltd. All rights reserved.
Private and Confidential.
www.d3technologies.co.uk
Company
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Formed in July 2007
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Major investment by Renishaw plc, a worldwide leader in imaging
& detection systems.
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Secure finances, avoiding the Venture Capitalist route
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R&D led, based at advanced research facility, Glasgow, UK
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Strong IP portfolio in our core technology based on Surface
Enhanced Resonance Raman Spectroscopy: SERRS
© 2008 D3 Technologies Ltd. All rights reserved.
Private and Confidential.
www.d3technologies.co.uk
Facilities
•3000 sq metre facility
•Bio-labs for DNA analysis; Class100 Clean Room;
Wet Chemistry, Colloid Prep and Spectroscopy
suites
© 2008 D3 Technologies Ltd. All rights reserved.
Private and Confidential.
www.d3technologies.co.uk
Surface Enhanced Raman Spectroscopy (SERS)
What is it?
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SERS is an analytical technique for trace levels of
soluble samples, providing information on molecular
structure, identity and quantity
© 2008 D3 Technologies Ltd. All rights reserved.
Private and Confidential.
www.d3technologies.co.uk
So Why SERS
So why SERS?
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Capable of detecting analytes in parts per billion range – extreme
sensitivity
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Directly applicably to wide sample range – flexible applications
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Target analytes give characteristic spectra – high specificity
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Short data collection times – rapid analysis
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Compatible with wide range of instrumentation – adaptable technology
D3 Technologies has extensive experience of using SERS based
methods for a wide range of applications, for everything from
explosives detection to clinical diagnostics.
D3 regularly undertake funded research programs for process
development in a variety of fields.
© 2008 D3 Technologies Ltd. All rights reserved.
Private and Confidential.
www.d3technologies.co.uk
Klarite® : substrates and technology
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Gold patterned
surfaces
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~mm wells
Semiconductor
fabrication
Proprietary
engineering
Customization
possible
Readiness
Performance
Features
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High reproducibility
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– RSD ~5%
– batch-to-batch
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>10 yrs shelf life
Demonstrated for
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commercially
available off-the-shelf
Certification in
progress (ISO 9001)
QMS/GMP
– Small molecules
– Proteins
– Spores/viruses
© 2008 D3 Technologies Ltd. All rights reserved.
Private and Confidential.
www.d3technologies.co.uk
Experiments using Klarite
pipette
SERS active area
sample
KlariteTM slide
1. Deposition
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2. Evaporation
3. Measurement
1-2uL solution.
Dilute solution to avoid bulk Raman: LOD as low as
1 ppb.
Limit of detection is analyte dependent.
© 2008 D3 Technologies Ltd. All rights reserved.
Private and Confidential.
www.d3technologies.co.uk
Bio-Pharmaceutical application – example
Intensity (arb.)
Ibuprofen - specific molecular 'fingerprint'
1180 cm-1
1205 cm-1
700
900
1100
1300
1500
1700
Wavenumber (cm-1)
ANALYSIS OF MIXTURES
MOLECULAR FINGERPRINT
2000
Peak @ 1180 cm-1
1800
10-4 M
10-5 M
10-6 M
Intensity (a.u.)
10-3 M
Peak Height Intensity (arb.)
1600
1400
1200
saturation
1000
800
600
400
1100
1150
1200
1250
1300
1350
Raman shift (cm-1)
200
Ibuprofen
0
0.1
0.01
0.001
0.0001
0.00001 0.000001
Concentration (M)
QUANTIFICATION
© 2008 D3 Technologies Ltd. All rights reserved.
Private and Confidential.
www.d3technologies.co.uk
Bio-Pharmaceutical application – example
© 2008 D3 Technologies Ltd. All rights reserved.
Private and Confidential.
www.d3technologies.co.uk
Questions?
© 2008 D3 Technologies Ltd. All rights reserved.
Private and Confidential.
www.d3technologies.co.uk