THE BECKMAN COULTER logo, COULTER, DxH, TruColor, and UniCel are trademarks of Beckman Coulter, Inc; COULTER and UniCel are registered in the USPTO. 1 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

2 of 53 Objectives  Software Overview  System & Details Status  Daily Checks & Shutdown  System Maintenance  Set-Up  Sample Processing  Cleaning Procedures  Sample-Flow Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Status Area

Software Overview

Alert Status Icons Utility Icons Global Icons and Navigation/Status Buttons System Icons

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Status Bar Local Navigation Bar

Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

System Status 4 of 53

Active

= online and ready

Inactive

= offline and not ready

Disabled

= maker or stainer not available for use

Enabled

= maker and/or stainer available for use

LIS Status: Green

= LIS Interface ON

Gray

= LIS Interface OFF Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Alert Status Icon color meaning: Red

= error

Yellow

= warning

Neutral

= OK Displays if unit is Online, Offline, in Diagnostics, or Stopped

Printer Status:

Displays

Auto Print Disabled

if

Disable All Auto Printed Reports Print

 is selected from

Print Printer Setup

Details Status 5 of 53

DATE & TIME of last DAILY CHECK and SHUTDOWN Cycles TRANSPORT STATUS TRANSFERS

Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

MAKER & STAINER STATUS START BUTTON DEFAULT SLIDE ORDER CASSETTE PREPARATION SINGLE-TUBE PREPARATION

Daily Checks & Shutdown 6 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

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Slidemaker Supplies Tab Setup Supplies

Supplies

Diluent 1 & 2 Cleaner Broken Slides Status Printer Ribbon Status Slide Status Replace Ribbon Empty Broken Slides Configure Containers

Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Supplies

Slidestainer Supplies Tab Bio Waste Status Slidestainer Baths

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Stain Protocol Reagents Setup Supplies Slidestainer Supplies Details Config Low Level View Log

Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Empty Basket Status Drawer Spaces Methanol Waste Status

Supplies - Details 9 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Loading Slides 10 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

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Setting up the Slidemaker 12 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Basket Settings SMS Configuration>Basket Settings 13 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Set Pre-Stain Drying Type SMS Configuration>Pre-Stain Drying 14 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Configure the Slide Label SMS>Configuration>Slide Label 15 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Set up Basket Location Availability SMS Configuration>Basket Location Availability 16 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Set up Stain Only Priority SMS Configuration>Stain-Only Priority 17 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Set up Agitation SMS>Configuration> Agitation Settings 18 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Set up Heater Availability SMS Configuration>Heater Availability 19 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Set up Smear Settings SMS Configuration> Smear Settings 20 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

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Setting up the Slidestainer 22 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Set Active Staining Protocol SMS Configuration>Staining Protocols 23 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Add/Edit a Staining Protocol SMS Configuration>Staining Protocols 24 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Configure Baths (Mapping) to the Bath Location 25 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Configure Baths (Mapping) to the Bath Location 26 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Configure Baths (Mapping) to the Bath Location 27 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

28 of 53 Copying a Staining Protocol • • • • • SMS Configuration>Staining Protocol Select a protocol Select “Copy Protocol” Re-Name the copy Select “OK” Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

29 of 53 Delete a Protocol • • • • SMS Configuration>Staining Protocol Select a protocol Select “Delete Protocol” Select “OK” Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

30 of 53 Quick Rinse Follow the procedure, when needed, to reduce stain precipitate on the slide for the Wright Giemsa QR (quick rinse) default stain protocol. Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

31 of 53 Disable/Enable the Slidemaker • • • • Select System Status>Details Status Select Module>Disable or Enable Maker Select “OK” Select “YES” Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

32 of 53 Disable/Enable the Slidestainer • • • • Select System Status>Details Status Select Module>Disable or Enable Stainer Select “OK” Select “YES” Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

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Running Samples Cassette Presentation • • Load the samples into the cassettes Place cassettes into the input buffer 34 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Running Samples Single Tube Presentation 35 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

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Basket Management System Status>Details Status>Basket Management 37 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Basket Details System Status>Details Status>Basket Management>Basket Details 38 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Advance Basket System Status>Details Status>Basket Management>Advance Basket 39 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Basket to Stain Next System Status>Details status>Basket Management 40 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Cancel Staining System Status>Details status>Basket Management 41 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

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Loading/Removing Baskets Input/Output (I/O) Drawer

The indicator lights in the I/O drawer mean the following:

• • • •

Blinking Green

– when the basket is ready for you to remove it

Steady Green Blinking Red

– when the basket is ready to be used by the system – when the basket is in an error state

Blinking Blue

– when a basket is loaded with slides for staining only 43 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Staining Samples Manually Stain-Only Priority 44 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

45 of 53 Making Slides (No Staining) • • • Select “Make Slide” on the Default Test Order for the applicable cassette or single tube preparation (OR) Add or edit a test from the Worklist Pending tab (OR) Load the samples for the processing of downloaded Test Orders from the LIS Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

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47 of 53 Cleaning Procedures • • • • • •

Daily

Daily Checks Shutdown Cleaning Stainer Baths and Tray (Daily or as Needed)

Weekly

Cleaning Baskets Clean Stainer Fill Probes, Drain Probes and Level Sense Probes

As Needed Procedures

For additional information on

As Needed Procedures

refer to Instructions For Use (IFU) Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

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Specimen Transport Module (STM) 49 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

UniCel DxH SlideMaker 50 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

UniCel DxH SlideStainer 51 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

Sample Aspiration Flow 52 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

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Any Questions?

Move your lab forward with Beckman Coulter.

Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.