Transcript Slide 1

Phytochemistry II
(206)
2014-2015
1.Caffeine
O
H3C
N
N
2,6 dioxy-purine
(Xanthine) alkaloid
1-Mayer’s test
0.5 ml alk soln
+ 4 dp Mayer’s reagent
O
CH3
N
CH3
No ppt
N
2- Special test (Murexide test)
Evaporate on B.W.B. to dryness
Residue
1ml alkaloid
soln
cool
+2dp conc HCL
+ 2dp H2O2
Evaporate
Violet
color
Invert on
NH3 vapors
Crimson
red color
Yellow
color
2-Quinine
1-Mayer’s test
Quinoline
alkaloid
0.5 ml of alkloid solution
4 drops of Mayer's Reagent
precipitat
e
Special tests for Quinine
I-Fluorescence test
Test
Blank
H2O to test
tube end
H2O to test
tube end
1ml dil H2SO4
1ml dil H2SO4
1ml alkaloid
Mix well & see against light
Blue fluorescence
II-Thaloquine test
2dps NH4 OH
2dps Bromine water
1ml alkaloid
Emerald green
III- Rosequine test
2dps NH4 OH
2dps K ferrocyanide
Bromine water till
yellow color
1ml alkaloid
Red color
3-Brucine
1-Mayer’s test
0.5ml of alk
4 drops of Mayer's Reagent
Indole
alkaloid
precipitate
2- Conc HNO3 test
A-Evaporate on BWB
1ml alkaloid
till complete dryness
solution
B- Cooling
C- 3 dps conc HNO3
D- See color I
E- Evaporate on
BWB till
complete dryness
F-See color II
of the residue
G- CooL
Violet color
H- 3 dps Of SnCl2
4-Morphine
1-Mayer’s test
Phenanthrene
alkaloid
0.5ml of alk soln
4 drops of Mayer's Reagent
Gelatinous
ppt.
2- Conc HNO3 test
See color II of
the residue
See color I
CooL
3 dps Of SnCl2
No Violet color
Special test for Morphine (Nitrous acid test)
1ml NaOH
1ml dil HCl
Few spikes of NaNO2
1ml alkaloid solution
Orange red color
5.Pilocarpine
Imidazole
alkaloid
1-Mayer’s test
0.5 ml alk soln
+ 4 dp Mayer’s reagent
precipitate
2- Conc HNO3 test :
A-Evaporate on BWB
1ml alkaloid
till complete dryness
solution
B- Cooling
colorless
C- 3 dps conc HNO3
D- See color I
E- Evaporate on
BWB till
complete dryness
F-See color II of
the residue
G- CooL
H- 3 dps alc KOH
No Violet color
colorless
3- Special test (Helch’s test)
1ml alk soln
+ 2dps H2O2
Shake
+ 2dp 1/300 K2Cr2O7
+ 1ml CHCl3
Yellow color
in aqueous layer
Violet color
in CHCl3 layer
Thank you