Transcript Powerpoint

The most important biological compounds are

polymers

Polymers

(poly =

many

)  The polymers are:

proteins, carbohydrates, lipids

(fats), and

nucleic acids

(DNA/RNA).

 A polymer is made up of a chain of many

monomers

linked together

MONOMERS

(mono = one)  Monomers are:

amino acids, sugars, fatty acids,

and

nucleotides.

 These are made

(dehydration synthesis)

down

(hydrolysis)

or broken over and over in living cells.

Large polymers are also called

_______________

Macromolecules are formed by by reactions involving the loss of water =

joined together during dehydration synthesis. Chains of monomers

Note

: enzymes that speed up dehydration synthesis reactions are called

(i.e. done by

adding

water to polymer).

Note

: enzymes that speed up hydrolysis reactions are called

http://science.nhmccd.edu/biol/dehydrat/dehydrat.html

Monomers (sub units) Polymers

a) b) c) d)

Polymers

Polymers

a) Carbohydrates b) c) d)

Polymers

a) Carbohydrates b) c) d)

Hydrolysis

Polymers

a) Carbohydrates b) c) d)

Hydrolysis H 2 O & Energy

Polymers

a) Carbohydrates b) c) d) a) b) c) d)

Monomers

Hydrolysis H 2 O & Energy

Polymers

a) Carbohydrates b) c) d)

Monomers

a) Simple sugars b) c) d)

Hydrolysis H 2 O & Energy

Polymers

a) Carbohydrates b) c) d)

Monomers

a) Simple sugars b) c) d)

Hydrolysis H 2 O & Energy

Polymers

a) Carbohydrates b) c) d)

Dehydration Synthesis

Monomers

a) Simple sugars b) c) d)

Hydrolysis H 2 O & Energy

H 2 O & Energy

Polymers

a) Carbohydrates b) c) d)

Dehydration Synthesis

Monomers

a) Simple sugars b) c) d)

Hydrolysis H 2 O & Energy

H 2 O & Energy

Polymers

a) Carbohydrates b) Proteins c) d)

Dehydration Synthesis

Monomers

a) Simple sugars b) c) d)

Hydrolysis H 2 O & Energy

H 2 O & Energy

Polymers

a) Carbohydrates b) Proteins c) d)

Dehydration Synthesis

Monomers

a) Simple sugars b) Amino Acids c) d)

Hydrolysis H 2 O & Energy

H 2 O & Energy

Polymers

a) Carbohydrates b) Proteins c) Lipids (fats) d)

Dehydration Synthesis

Monomers

a) Simple sugars b) Amino Acids c) d)

Hydrolysis H 2 O & Energy

H 2 O & Energy

Polymers

a) Carbohydrates b) Proteins c) Lipids (fats) d)

Dehydration Synthesis

Monomers

a) Simple sugars b) Amino Acids c) Fatty Acids & Glycerol d)

Hydrolysis H 2 O & Energy

H 2 O & Energy

Polymers

a) Carbohydrates b) Proteins c) Lipids (fats) d) DNA/RNA (nucleic acids)

Dehydration Synthesis

Monomers

a) Simple sugars b) Amino Acids c) Fatty Acids & Glycerol d)

Hydrolysis H 2 O & Energy

H 2 O & Energy

Polymers

a) Carbohydrates b) Proteins c) Lipids (fats) d) DNA/RNA (nucleic acids)

Dehydration Synthesis

Monomers

a) Simple sugars b) Amino Acids c) Fatty Acids & Glycerol d) Nucleotides

Hydrolysis H 2 O & Energy

H 2 O & Energy Dehydration Synthesis

Polymers

a) Carbohydrates b) Proteins c) Lipids (fats) d) DNA/RNA (nucleic acids) These reactions require: 1.

Monomers

a) Simple sugars b) Amino Acids c) Fatty Acids & Glycerol d) Nucleotides

Hydrolysis H 2 O & Energy

H 2 O & Energy Dehydration Synthesis

Polymers

a) Carbohydrates b) Proteins c) Lipids (fats) d) DNA/RNA (nucleic acids) These reactions require: 1. ATP energy

Monomers

a) Simple sugars b) Amino Acids c) Fatty Acids & Glycerol d) Nucleotides

Hydrolysis H 2 O & Energy

H 2 O & Energy Dehydration Synthesis

Polymers

a) Carbohydrates b) Proteins c) Lipids (fats) d) DNA/RNA (nucleic acids) These reactions require: 1. ATP energy 2. Water

Monomers

a) Simple sugars b) Amino Acids c) Fatty Acids & Glycerol d) Nucleotides

Hydrolysis H 2 O & Energy

H 2 O & Energy

Polymers

a) Carbohydrates b) Proteins c) Lipids (fats) d) DNA/RNA (nucleic acids)

Dehydration Synthesis

These reactions require: 1. ATP energy 2. Water 3. Enzymes

Monomers

a) Simple sugars b) Amino Acids c) Fatty Acids & Glycerol d) Nucleotides

Hydrolysis H 2 O & Energy

Where does the name come from?

Hydrated Carbons:

(CH 2 0)n

Carbohydrates have the empirical formula of (CH 2 0)n where n = the # of times the chain is repeated.

The carbons, hydrogens and oxygens are found in the ratio of

1:2:1

and are made up of a repeating chain of sugars.

(CH 2 0) 3 =

C

3

H

6

0

3

(CH 2 0) 6 =

C

6

H

12

0

6

Sugars are also known as

saccarides

.

Carbohydrates usually end in ‘

ose

’ . Can you think of any examples?

The basic sugar molecule is

GLUCOSE

:

C

6

H

12

O

6 .

Glucose has a

ring

structure. Other monosaccharides include

fructose, ribose, deoxyribose

6 sided = HEXOSE C 6 H 12 O 6 5 sided = PENTOSE C 6 H 12 O 6

When two sugars bind together via

DEHYDRATION SYNTHESIS

a disaccharide is formed.

glucose + glucose forms the sugar

maltose

glucose + fructose forms the sugar

sucrose

galactose + glucose forms the sugar

lactose

• • • • When many sugars bind together via dehydration synthesis four types of polysaccharides may be formed:

Starch Glycogen Cellulose Chitin

• The

cell walls

of plants are made of cellulose • They are

long chains

of glucose molecules with

no side chains

.

• • The linkage between the Carbon atoms of the sugars is different than starch and glycogen No mammal can break this bond • 5. This is why we

cannot digest

cellulose =

FIBRE

.

Plants store their energy

as starch • Starch is made up of many glucose molecules linked together • Starch has

few side chains

Animals store their energy

(extra glucose) as glycogen • We store glycogen in our

liver and muscles

• Glycogen is made up of many glucose molecules linked together • Glycogen has

many side chains

• Made by

animals and fungi

• Long glucose chains linked with

covalent bonds

. • Very

strong

• Makes structures like

exo-skeletons

, fingernails,

claws

, and beaks

1.

Energy

: when the

bonds

between Carbon atoms

are broken

, the energy

released

can be used by cells. Carbohydrates are the primary energy molecules for all life.

2.

Structural

: Cellulose is the major structural compound in plants (is used in the

cell wall

).

Lipids are made up of the elements

C,H,O

but in no set ratio. Lipids are large molecules that are

insoluble in water

.

Synthesis of a FAT animation: http://www2.nl.edu/jste/lipids.htm

1. Composed of

3 fatty acids

bonded to

1 glycerol

. 2. Fatty acids contain a

long chain

of 16-18 Carbons with an acid end.

3. Glycerol is a

small 3 Carbon chain

with 3 alcohol (OH) groups 4. These two molecules bind together via dehydration synthesis

1. Saturated fats :

There are

no double bonds

fatty acids. in the carbon chains of the The carbons are filled with

hydrogens

.

Unhealthy

. They mostly come from

animals

.

Become

solid

at room temperature. Examples: lard,

butter

, animal fats…

2. Unsaturated fats :

There are one (

monounsaturated

) or more

double bonds

(

polyunsaturated

). Mostly come from

plants

. They are

liquid

at room temperature.

Healthy

Examples:

olive oil

, corn oil, palm oil…

Are used to make up the two layered

cell membrane

of all cells.

In phospholipids, the

third fatty acid

group of a triglyceride is

replaced by

an inorganic

phosphate group

(

PO 4 3-

).

This creates a

polar end

: The

phosphate

end is water soluble (

hydrophilic

) The

fatty acid

is not water soluble (

hydrophobic

)

hydrophobic hydrophilic

Steroids structurally look very different from lipids, but are also

water insoluble

. They are made up of

4 Carbon ring

molecules fused together. Examples:

testosterone

, estrogen,

cholesterol

, and vitamin

D

. Used as

sex hormones

1. Long term storage for energy

(more efficient spacewise than glycogen or starch).

2. Insulation

and

protection

in animals 3. Making some

hormones

(steroids) 4. Structure of

cell membranes

. Without lipids, we would have no cells.

• Found in fish and leafy vegetables • Other foods are now offering omega-3 ’ s (eggs, cereals, margarine…) • Help to reduce cancer • Helps with vision • Helps us think better

Scientific evidence has shown that dietary saturated and trans fats can increase your risk of developing heart disease.

1. Proteins are made up of the elements

C,H,O, and N

(but in no set ratio). 2. Proteins are chains of

Amino Acids

(usually

75 or more

) that bond together via dehydration synthesis.

3. 40%

of the average human body is made up of protein.

1. The building blocks of Proteins are amino acids.

2. There are

three parts

to an amino acids:

1. Amino Group

H + ) (NH 2 or NH 3 + ) acts as a base (accepts

2. Carboxyl Group

(donates H + ) (COOH or COO ) acts as an acid

3. R Group

: there are

20

different possible R groups

Amino acids bond together via dehydration synthesis.

The amino acids bind together with a

peptide bond

.

The PEPTIDE bond is formed between

C and N

and one water is lost (dehydration synthesis).

When the original two amino acids form the beginning of the chain (with one peptide bond) it is called a

DIPEPTIDE

.

Then the chain grows to become a

TRIPEPTIDE

.

Ultimately you end up with a

POLYPEPTIDE

(which can have anywhere between

30 and 30,000

amino acids). Another name for a polypeptide is

protein

.

Every protein is different because the

ORDER of amino acids

is different.

The chains come together differently due to the

order of the different R groups

and

how they bond

together. This structural difference also makes the polypeptides (proteins) functionally different.

This is the

first level

proteins are formed. of how It is simply the

order of amino acids

joined together with peptide bonds. It is the amino acid sequence that determines the nature and chemistry of the protein. If you change the order of amino acids, the protein may not be able to

do its job

.

This is the second step in the formation of a protein. When a peptide bond is formed, a double bonded oxygen is left over, which is partially

negative

(the carboxyl group:

COO -

). It is attracted to the

positive NH 3 +

acids in the chain. amino group from other amino This attraction forms a

HYDROGEN BOND

.

This causes the chain to twist into either a spiral called an

alpha helix

or a

beta pleated sheet

.

The next interactions take place

between the R groups

. Some R groups are

reactive

and will interact with other reactive R groups in the chain. These are the amino acids that are either

charged

or that have a

sulphur atom

.

The interactions (

+ and –

attractions and

S-S bridges

) will fold the molecule over into a highly

specific 3-dimensional shape

. It is the 3-D shape that will determine the protein ’ s the body.

job

or role in

The last level in protein formation is

not seen

in all proteins. However, some proteins are actually

2 or more

molecules joined to form a functional protein. They are held together with an

ionic bond

.

Two examples:

Insulin

has

2

subunits

Hemoglobin

has

4

subunits.

Peptide

Bonds Hydroge

n

Bonds Interactions between

R groups Ionic

Bonds

The final shape of a protein (its tertiary or quaternary structure) is very

specific

and enables it to do its job/function. Any

change

in a proteins ’

shape

will affect its

function

.

Denaturation is when a protein's tertiary structure is lost. This happens when the

bonds between the R groups are broken

. When a protein is denatured, the protein can ’ t do its job and becomes

useless

.

How can this happen? There are three common ways:

1. Temperature

:

High temperatures affect the weak Hydrogen bonds and can

distort or break

them, thus changing the structural shape. A slight increase in temperature an cause a

reversible

change (ie: fever). A high temperature increase can cause an

irreversible

(ie: cooking an egg). change

How can this happen? There are three common ways:

2.

Chemicals

:

Heavy metals such as

lead and mercury

are large atoms that are attracted the R groups of amino acids. They

bond to the R group

the protein ’ s shape.

and distort This is usually

irreversible

don ’ t want to ‘ let go ’ ).

(they usually

How can this happen? There are three common ways:

3.

pH

:

As some of the R groups are acids and some are bases, every protein (enzyme) has a

preferred pH

. Any change in pH causes a

change in the acid-base R group

interactions and this will change the

shape

of the protein.

1. Structural: proteins help make up all structures in living things Actin & Myosin:

muscle

proteins

Keratin

: nails, hair, horns, feathers

Collagen

: bones, teeth, cartilage, tendon, ligament, blood vessels, skin matrix

2. Functional:

other proteins help us to keep our bodies

functioning properly

and to

digest our food

.

Enzymes :

are proteins that are

catalysts

which speed up reactions and control all cell activities.

Hemoglobin

3. Food Source :

once we have used up all of our carbohydrates and fats, proteins will be used for energy.

Proteins are worth the

least amount of energy

per gram.

Anorexia and Bolimia

Nucleic acids are

acidic

molecules that are found in the

nucleus

of cells. There are two types, both of which are very

LARGE

. 1. DNA:

Deoxyribonucleic Acid

2. RNA:

Ribonucleic Acid

All nucleic acids are composed of units called

NUCLEOTIDES

, which are composed of three sub-molecules: 1.

Pentose Sugar

(ribose or deoxyribose) 2.

Phosphate

3.

Nitrogen Base

(purine or pyrimidine)

They are formed by joining their subunits together via dehydration synthesis (nucleotide + nucleotide … = nucleic acid).

This is quite a

complex

process to which we will devote an entire unit to.

Adenine and Guanine

Have

two

rings

Found in both DNA and RNA Memory Trick:

It ’ s Got 2 Be GAP

Uracil

Cytosine , Thymine , and Uracil

Have only

one

ring Cytosine is in

both

DNA and RNA Thymine is in

DNA only

Uracil is in

RNA only Memory Trick:

CUT the Pyramid

Structure of DNA:

DNA is composed of two complimentary strands of nucleotides. The two strands are joined by

hydrogen bonds

which form between

complimentary

nitrogen bases:

Adenine with Thymine (A-T or T-A)

They join with

2

hydrogen bonds

Cytosine with Guanine (C-G or G-C)

They join with

3

hydrogen bonds

When DNA is first made, it is just

two linear strands

of nucleotides joined together.

Due to internal bonding, the DNA molecule then forms into a

double helix

(twisted ladder).

a) Directs and controls all cell activities

by making all of the

proteins

and

enzymes

b) Contains all of the

genetic information

necessary to make one complete organism of very exact specifications

RNA is made by

DNA

.

It is not confined to the nucleus, it moves out of the nucleus

into the cytoplasm

of the cell.

It has

Ribose

sugar instead of Deoxyribose. It has no

thymines

, and uses

URACIL

s

instead. It is

single stranded

and therefore, no helix is formed. There are

3 types

of RNA.

The function of RNA is to

assist DNA in making proteins

.

DNA

Nitrogen bases: A,T,G,C Sugar: deoxyribose Double stranded 1 type Found in the nucleus only Forms a double helix DNA makes DNA Very big molecule

RNA

Nitrogen bases: A, U, G, C Sugar: ribose Single stranded 3 types: a) mRNA – messenger b) tRNA – transfer c) rRNA – ribosomal Found in the nucleus and the cytoplasm No helix DNA makes RNA Much smaller molecule

ATP is also thought of as a

nucleic acid

as it has the same

structure

as a nucleotide. The only difference is that it has

THREE phosphate groups

instead of one.

This is the

energy source

for the body.

Our

mitochondria

turn the energy of

glucose

into

ATP

.

Why is it a good molecule to store energy? It takes a lot of

energy

to put two phosphate molecules together (both – ’ ve). So when you

break that bond

, a lot of energy is

released

.

C6H12O6 + 6O2 -----> 6CO2 + 6H20 + energy (heat and ATP)