Extraction of Nicotine
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Transcript Extraction of Nicotine
Extraction of Nicotine
By: DJ Hall and Brittany Hicks
Purpose
Determine if nicotine is extractable by means of
supercritical fluid
Run more than one sample type to determine if this
makes a significant difference
SFE
Supercritical Fluid Extraction
Uses high pressure CO2
Used to extract organics from different types of matrixes
Allows for easy separation
Problems
All soluble organics will be separated form matrix
GC-MS
Gas Chromatography Mass Spectroscopy
Separation of violate organics
Determine the analyte separated at specific retention
times using MS
Nicotine (162.12g/mol)
Poison (parasympathomimetic alkaloid)
Formed in nightshade family of plants
Antiherbivor chemical
Small doses
Stimulant
Relaxation
Large doses
Fatal
Extraction
2 Cigarettes Marlboro blend 27
2 Pieces Nicorette Gum 2mg
50 Drops of e-cigarette liquid 2.4% by weight
Extraction cont.
All extractions were obtained by bubbling into methanol
and using SFE methanol modifier pump
Cigarettes
Ground using mortar and pestle
Nicorette Gum
Cut into small pieces
E-cigarette liquid
Celite as inert matrix
Determination of Nicotine
All samples were diluted to 50ml of methanol
A standard was created by diluting 4mg of Nicotine in
50ml of methanol
Standards and Samples were run using the GC-MS
Reference Mass Spectra
Standard
Nicotine peak
Cigarettes
Nicotine peak
Cigarettes cont.
E-Cigarette Liquid
Nicotine peak
E-Cigarette Liquid Cont.
Nicorette Gum
Chromatogram Comparison
E-Cigarette Liquid
Nicorette Gum
Cigarette
Standard
Conclusion
It is possible to extract nicotine using the SFE
Both the E-Cigarette and Cigarette samples contained
nicotine
The Nicorette Gum showed undetectable amounts of
nicotine
Nicotine may have be contained in the gum residue that
was filtered before dilution
Future Project
Quantify the amounts of nicotine extracted
Determine the extraction efficiency
Work Cited
Fischer, M. and Jefferies, T. Optimization of Nicotine
Extraction from Tobacco Using Supercritical Fluid
Technology with Dynamic Extraction Modeling. J.Agric.
Food Chem. (1996), 44, 1258-1264.