Transcript Ch07 Lecture-The Cell Cycle and Cell Division
7
The Cell Cycle and Cell Division
Chapter 7 The Cell Cycle and Cell Division
Key Concepts
• 7.1 Different Life Cycles Use Different Modes of Cell Reproduction • 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells • 7.3 Cell Reproduction Is Under Precise Control
Chapter 7 The Cell Cycle and Cell Division
Key Concepts
• 7.4 Meiosis Halves the Nuclear Chromosome Content and Generates Diversity • 7.5 Programmed Cell Death Is a Necessary Process in Living Organisms
Chapter 7 Opening Question How does infection with HPV result in uncontrolled cell reproduction?
Concept 7.1 Different Life Cycles Use Different Modes of Cell Reproduction The lifespan of an organism is linked to
cell reproduction
—
usually called cell division.
Organisms have two basic strategies for reproducing themselves: • Asexual reproduction • Sexual reproduction Cell division is also important in growth and repair of tissues.
Figure 7.1 The Importance of Cell Division (Part 1)
Figure 7.1 The Importance of Cell Division (Part 2)
Figure 7.1 The Importance of Cell Division (Part 3)
Concept 7.1 Different Life Cycles Use Different Modes of Cell Reproduction In
asexual reproduction
the offspring are
clones
—genetically identical to the parent.
Any genetic variations are due to
mutations
.
A unicellular prokaryote may reproduce itself by
binary fission.
Single-cell eukaryotes can reproduce by
mitosis.
Other eukaryotes are also able to reproduce through asexual or sexual means.
Figure 7.2 Asexual Reproduction on a Large Scale
Concept 7.1 Different Life Cycles Use Different Modes of Cell Reproduction
Sexual reproduction
requires
gametes
—two parents each contribute one gamete to an offspring. Gametes form by
meiosis
—a process of cell division.
Gametes —and offspring—differ genetically from each other and from the parents.
Concept 7.1 Different Life Cycles Use Different Modes of Cell Reproduction DNA in eukaryotic cells is organized into
chromosomes
.
A
chromosome
consists of a single molecule of DNA and proteins.
Somatic cells
—body cells not specialized for reproduction Each somatic cell contains
two sets of chromosomes (homologs)
homologous pairs.
that occur in
Concept 7.1 Different Life Cycles Use Different Modes of Cell Reproduction Gametes contain only
one
set of chromosomes —one homolog from each pair.
Haploid
cell —Number of chromosomes =
n
Fertilization
—Two haploid gametes (female egg and male sperm) fuse to form a
zygote
.
Chromosome number in zygote = 2
n
and cells are
diploid
.
Concept 7.1 Different Life Cycles Use Different Modes of Cell Reproduction All kinds of sexual life cycles involve meiosis:
Haplontic
life cycle —in protists, fungi, and some algae —zygote is only diploid stage After zygote forms it undergoes meiosis to form haploid
spores
, which germinate to form a new organism.
Organism is haploid, and produces gametes by mitosis —cells fuse to form diploid zygote.
Figure 7.3 All Sexual Life Cycles Involve Fertilization and Meiosis (Part 1)
Concept 7.1 Different Life Cycles Use Different Modes of Cell Reproduction
Alternation of generations
—most plants, some protists; meiosis gives rise to haploid spores Spores divide by mitosis to form the haploid generation (
gametophyte
).
Gametophyte forms gametes by mitosis. Gametes then fuse to form diploid zygote (
sporophyte
), which in turn produces haploid spores by meiosis.
Figure 7.3 All Sexual Life Cycles Involve Fertilization and Meiosis (Part 2)
Concept 7.1 Different Life Cycles Use Different Modes of Cell Reproduction
Diplontic
life cycle —animals and some plants; gametes are the only haploid stage A mature organism is diploid and produces gametes by meiosis.
Gametes fuse to form diploid zygote; zygote divides by mitosis to form mature organism.
Figure 7.3 All Sexual Life Cycles Involve Fertilization and Meiosis (Part 3)
Concept 7.1 Different Life Cycles Use Different Modes of Cell Reproduction The essence of sexual reproduction is that it allows the
random selection of half the diploid chromosome set.
This forms a haploid gamete that fuses with another to make a diploid cell.
Thus, no two individuals have exactly the same genetic makeup.
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells Four events must occur for cell division: •
Reproductive signal
—to initiate cell division •
Replication
of DNA •
Segregation
—distribution of the DNA into the two new cells •
Cytokinesis
—division of the cytoplasm and separation of the two new cells
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells In prokaryotes, cell division results in reproduction of the entire organism.
The cell: • Grows in size • Replicates its DNA • Separates the DNA and cytoplasm into two cells through
binary fission
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells Most prokaryotes have one chromosome, a single molecule of DNA —usually
circular
.
Two important regions in reproduction: •
ori
- where replication starts •
ter
- where replication ends
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells Replication occurs as the DNA is threaded through a “replication complex” of proteins in the center of the cell.
Replication begins at the
ori
towards the
ter
site.
site and moves
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells As replication proceeds, the
ori
complexes move to opposite ends of the cell.
DNA sequences adjacent to the
ori
region actively bind proteins for the segregation, hydrolyzing ATP for energy.
An actin-like protein provides a filament along which
ori
and other proteins move.
Figure 7.4 Prokaryotic Cell Division
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells Cytokinesis begins after chromosome segregation by a pinching in of the plasma membrane —protein fibers form a ring.
As the membrane pinches in, new cell wall materials are synthesized resulting in separation of the two cells.
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells Eukaryotic cells divide by mitosis followed by cytokinesis.
Replication
of
DNA
occurs as long strands are threaded through replication complexes.
DNA replication only occurs during a specific stage of the cell cycle.
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells In
segregation of DNA
after cell division, one copy of each chromosome ends up in each of the two new cells.
In eukaryotes, the chromosomes become highly condensed.
Mitosis
segregates them into two new nuclei — the cytoskeleton is involved in the process.
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells
Cytokinesis
follows mitosis.
The process in plant cells (which have cell walls) is different than in animal cells (which do not have cell walls).
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells The
cell cycle
—the period between cell divisions In eukaryotes it is divided into mitosis and cytokinesis —called the M phase—and a long interphase.
During
interphase
, the cell nucleus is visible and cell functions including replication occur Interphase begins after cytokinesis and ends when mitosis starts.
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells Interphase has three subphases: G1, S, and G2.
G1
(
Gap 1
) —variable, a cell may spend a long time in this phase carrying out its functions
S phase
(
Synthesis
) —DNA is replicated
G2
(
Gap 2
) —the cell prepares for mitosis, synthesizes microtubules for segregating chromosomes
Figure 7.5 The Phases of the Eukaryotic Cell Cycle (Part 1)
Figure 7.5 The Phases of the Eukaryotic Cell Cycle (Part 2)
Figure 7.5 The Phases of the Eukaryotic Cell Cycle (Part 3)
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells In mitosis,
one nucleus produces two daughter nuclei each containing the same number of chromosomes as the parent nucleus
. Mitosis is continuous, but can be can be divided into phases —prophase, prometaphase, metaphase, anaphase, and telophase.
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells During interphase, only the nuclear envelope and and the nucleolus are visible.
The
chromatin
(DNA) is not yet condensed.
Three structures appear in
prophase:
• The condensed chromosomes • Centrosome • Spindle
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells
Condensed chromosomes
appear during prophase.
Sister chromatids
—two DNA molecules on each chromosome after replication
Centromere
—region where chromatids are joined
Kinetochores
are protein structures on the centromeres, and are important for chromosome movement.
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells The
karyotype
of an organism reflects the number and sizes of its condensed chromosomes.
Karyotype analysis can be used to identify organisms, but DNA sequence is more commonly used.
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells Segregation is aided by other structures: The
centrosome
determines the orientation of the spindle apparatus.
Each centrosome can consist of two
centrioles
—hollow tubes formed by microtubules.
Centrosome is duplicated during S phase and each moves towards opposite sides of the nucleus.
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells Centrosomes serve as
mitotic centers
or
poles
; the
spindle
forms between the poles from two types of microtubules: •
Polar microtubules
in the center.
form a spindle and overlap •
Kinetochore microtubules
—attach to kinetochores on the chromatids. Sister chromatids attach to opposite halves of the spindle.
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells Chromosome separation and movement is highly organized.
During
prometaphase
, the nuclear envelope breaks down.
Chromosomes consisting of two chromatids attach to the kinetochore mictotubules.
Figure 7.6 The Phases of Mitosis (1)
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells Durin
metaphase
, chromosomes line up at the midline of the cell.
During
anaphase
, the separation of sister chromatids is controlled by M phase cyclin Cdk; cohesin is hydrolyzed by
separase.
After separation, they move to opposite ends of the spindle and are referred to as
daughter chromosomes
.
Figure 7.6 The Phases of Mitosis (2)
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells A protein at the kinetochores —
cytoplasmic dynein
—hydrolyzes ATP for energy to move chromosomes along the microtubules towards the poles.
Microtubules also shorten, drawing chromosomes toward poles.
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells
Telophase
separated: occurs after chromosomes have • Spindle breaks down • Chromosomes uncoil • Nuclear envelope and nucleoli appear • Two daughter nuclei are formed with identical genetic information
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells Cytokinesis: Division of the cytoplasm differs in plant and animals • In animal cells, plasma membrane pinches between the nuclei because of a
contractile ring
of microfilaments of actin and myosin.
Figure 7.7 Cytokinesis Differs in Animal and Plant Cells (Part 1)
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells Plant cells: Vesicles from the Golgi apparatus appear along the plane of cell division • These fuse to form a new plasma membrane.
• Contents of vesicles form the
cell plate
—the beginning of the new cell wall.
Figure 7.7 Cytokinesis Differs in Animal and Plant Cells (Part 2)
Concept 7.2 Both Binary Fission and Mitosis Produce Genetically Identical Cells After cytokinesis:
Each daughter cell contains all of the components of a complete cell
.
Chromosomes are precisely distributed.
The
orientation
of cell division is important to development, but organelles are not always evenly distributed.
Concept 7.3 Cell Reproduction Is Under Precise Control The reproductive rates of most prokaryotes respond to environmental conditions. In eukaryotes, cell division is related to the needs of the entire organism.
Cells divide in response to extracellular signals, like
growth factors
.
Concept 7.3 Cell Reproduction Is Under Precise Control The eukaryotic cell cycle has four stages: G1, S, G2, and M.
Progression is tightly regulated —the
G1-S
transition is called
R
, the
restriction point
.
Passing this point usually means the cell will proceed with the cell cycle and divide.
Figure 7.8 The Eukaryotic Cell Cycle
Concept 7.3 Cell Reproduction Is Under Precise Control Specific signals trigger the transition from one phase to another.
Evidence for substances as triggers came from
cell fusion
experiments.
Nuclei in cells at different stages, fused by polyethylene glycol, both entered the phase of DNA replication (S).
Figure 7.9 Regulation of the Cell Cycle (Part 1)
Concept 7.3 Cell Reproduction Is Under Precise Control Transitions also depend on activation of
cyclin dependent kinases
(
Cdk’s
). A
protein kinase
is an enzyme that catalyzes phosphorylation from ATP to a protein.
Phosphorylation changes the shape and function of a protein by changing its charges.
Concept 7.3 Cell Reproduction Is Under Precise Control Cdk is activated by binding to
cyclin
(by
allosteric regulation
); this alters its shape and exposes its active site.
The G1-S cyclin-Cdk complex acts as a protein kinase and triggers transition from G1 to S.
Other cyclin Cdk’s act at different stages of the cell cycle, called
cell cycle checkpoints
.
Figure 7.10 Cyclins Are Transient in the Cell Cycle
Concept 7.3 Cell Reproduction Is Under Precise Control Example of G1-S cyclin-Cdk regulation: Progress past the restriction point in G1 depends on
retinoblastoma protein (RB
).
RB normally inhibits the cell cycle, but when phosphorylated by G1-S cyclin-Cdk, RB becomes inactive and no longer blocks the cell cycle.
Concept 7.4 Meiosis Halves the Nuclear Chromosome Content and Generates Diversity
Meiosis
consists of
two
nuclear divisions but
DNA is replicated only once
. The function of meiosis is to: • Reduce the chromosome number from diploid to haploid • Ensure that each haploid has a complete set of chromosomes • Generate diversity among the products
Figure 7.11 Mitosis and Meiosis: A Comparison
Figure 7.12 Meiosis: Generating Haploid Cells (Part 1)
Figure 7.12 Meiosis: Generating Haploid Cells (Part 2)
Figure 7.12 Meiosis: Generating Haploid Cells (Part 3)
Figure 7.12 Meiosis: Generating Haploid Cells (Part 4)
Figure 7.12 Meiosis: Generating Haploid Cells (Part 5)
Concept 7.4 Meiosis Halves the Nuclear Chromosome Content and Generates Diversity Meiotic division reduces the chromosome number. Two unique features: • In
meiosis I
,
homologous pairs of chromosomes come together and line up
along their entire lengths.
• After metaphase I,
the homologous chromosome pairs separate
, but individual chromosomes made up of two sister chromatids remain together.
Concept 7.4 Meiosis Halves the Nuclear Chromosome Content and Generates Diversity Meiosis I is preceded by an S phase during which DNA is replicated.
Each chromosome then consists of two sister chromatids, held together by cohesin proteins.
At the end of meiosis I, two nuclei form, each with half the original chromosomes —still composed of sister chromatids.
Concept 7.4 Meiosis Halves the Nuclear Chromosome Content and Generates Diversity Sister chromatids separate during
meiosis II
, which is
not
proceeded by DNA replication.
The products of meiosis I and II are four cells with a haploid number of chromosomes.
These four cells are not genetically identical
.
Two processes may occur: Crossing over and independent assortment
Concept 7.4 Meiosis Halves the Nuclear Chromosome Content and Generates Diversity In prophase of meiosis I homologous chromosomes pair by
synapsis
.
The four chromatids of each pair of chromosomes form a
tetrad
,or
bivalent
.
The homologs seem to repel each other but are held together at
chiasmata.
Concept 7.4 Meiosis Halves the Nuclear Chromosome Content and Generates Diversity
Crossing over
is an
exchange of genetic material
that occurs at the chiasma.
Crossing over results in
recombinant
chromatids and increases genetic variability of the products.
In-Text Art, Ch. 7, p. 138
Figure 7.13 Crossing Over Forms Genetically Diverse Chromosomes
Concept 7.4 Meiosis Halves the Nuclear Chromosome Content and Generates Diversity Prophase I may last a long time.
• Human males: Prophase I lasts about 1 week, and 1 month for entire meiotic cycle • Human females: Prophase I begins before birth, and ends up to decades later during the monthly ovarian cycle
Concept 7.4 Meiosis Halves the Nuclear Chromosome Content and Generates Diversity
Independent assortment
during anaphase I also allows for chance combinations and genetic diversity.
After homologous pairs of chromosomes line up at metaphase I, it is a matter of chance which member of a pair goes to which daughter cell.
The more chromosomes involved, the more combinations possible.
Concept 7.4 Meiosis Halves the Nuclear Chromosome Content and Generates Diversity Meiotic errors:
Nondisjunction
—homologous pairs fail to separate at anaphase I —sister chromatids fail to separate, or homologous chromosomes may not remain together Either results in
aneuploidy
—chromosomes lacking or present in excess
Concept 7.4 Meiosis Halves the Nuclear Chromosome Content and Generates Diversity Organisms with triploid (3
n
), tetraploid (4
n
), and even higher levels are called
polyploid
.
This can occur through an extra round of DNA duplication before meiosis, or the lack of spindle formation in meiosis II.
• Polyploidy occurs naturally in some species, and can be desirable in plants.
Concept 7.4 Meiosis Halves the Nuclear Chromosome Content and Generates Diversity If crossing over happens between
non homologous chromosomes
, the result is a
translocation
.
A piece of chromosome may rejoin another chromosome, and its location can have profound effects on the expression of other genes.
Example: Leukemia
In-Text Art, Ch. 7, p. 140
Concept 7.5 Programmed Cell Death Is a Necessary Process in Living Organisms Cell death occurs in two ways: • In
necrosis
, the cell is damaged or starved for oxygen or nutrients. The cell swells and bursts.
Cell contents are released to the extracellular environment and can cause inflammation.
7.5 ProConcept 7.ammed Cell Death Is a Necessary Process in Living Organisms •
Apoptosis
is genetically programmed cell death. Two possible reasons:
The cell is no longer needed
, e.g., the connective tissue between the fingers of a fetus.
Old cells may be prone to genetic damage that can lead to cancer
—blood cells and epithelial cells die after days or weeks.
Concept 7.5 Programmed Cell Death Is a Necessary Process in Living Organisms Events of apoptosis: • Cell detaches from its neighbors • Cuts up its chromatin into nucleosome-sized pieces • Forms membranous lobes called “blebs” that break into fragments • Surrounding living cells ingest the remains of the dead cell
Figure 7.14 Apoptosis: Programmed Cell Death (Part 1)
Concept 7.5 Programmed Cell Death Is a Necessary Process in Living Organisms Cell death cycle is controlled by signals: • Lack of a mitotic signal (growth factor) • Recognition of damaged DNA External signals cause membrane proteins to change shape and activate enzymes called
caspases
—hydrolyze proteins of membranes.
Figure 7.14 Apoptosis: Programmed Cell Death (Part 2)
Answer to Opening Question Human papilloma virus (HPV) stimulates the cell cycle when it infects the cervix.
Two proteins regulate the cell cycle:
Oncogene
proteins are positive regulators of the cell cycle —in cancer cells they are overactive or present in excess
Tumor suppressors
are negative regulators of the cell cycle, but in cancer cells they are inactive —can be blocked by a virus such as HPV
Figure 7.15 Molecular Changes Regulate the Cell Cycle in Cancer Cells