Transcript See Power Point Presentation.

Objectives

To determine the properties and quality of
the horse joint supplements Legend and
Hyalovet by:
•Using the rheometers to measure the
viscosity and elasticity.
•Finding the intrinsic viscosity using capillary
viscometers.
Rheometers
Rate Control
Strain Control
Viscometer

Timing
Lines
Capillary
Glass tubes that can
be used to calculate
the intrinsic viscosity
of polymer solutions
by measuring the
rate at which a liquid
falls through the
capillary at different
dilutions.
Hyaluronic Acid
•Biopolyelectrolyte
•High molecular weight
•viscoelastic properties
•costs 100,000 per kilogram
Sources of HA
•rooster combs
•micro-organisms
•umbilical cords
Intrinsic Viscosities of HA Supplements in
PBS
2000.00
1800.00
1600.00
Intrinsic Viscosity = 1074 ml/g
(T-TS)/(TS*C)
1400.00
IV = 534
IV = 531
(ml/g)
Trial 1 Hyalovet
1200.00
Trial 2 Hyalovet
1000.00
Trial 1 Legend
IV = 991 ml/g
800.00
Trial 2 Legend
Linear (Trial
Hyalovet)
Linear (Trial
Hyalovet)
Linear (Trial
Legend)
Linear (Trial
Legend)
600.00
400.00
200.00
0.00
0.00E+00
2.00E-04
4.00E-04
6.00E-04
8.00E-04
Concentration HA (mg/ml)
1.00E-03
1.20E-03
2
1
2
1
Moduli of Legend and Hyalovet
10.00
Moduli (Pa)
1.00
Legend Elastic Modulus (Pa)
Legend Viscous Modulus (Pa)
Hyalovet Elastic Modulus (Pa)
0.10
Hyalovet Viscous Modulus (Pa)
0.01
0.00
0.10
1.00
Frequency (hz)
10.00
Viscosity of Legend and Hyalovet
Viscosity (Pas)
1.00
Legend Viscosity
0.10
0.01
0.10
Hyalovet Viscosity
1.00
10.00
Shear Rate (1/s)
100.00
1000.00
They Came to Tame the Lame
Conclusions



The experiments performed on the
rheometer showed similar readings for the
flow of Legend and Hyalovet
Legend and Hyalovet showed the same
viscous and elastic moduli.
The intrinsic viscosity of Hyalovet is twice
that of Legend which shows that Hyalovet
has a larger molecular weight.