Transcript Pig Dissection
Fetal Pig Dissection!
• Work in Groups of 3-4 • Get out lab books and lab packets!
Fetal Pig Dissection – Day 1
Getting Started!
Obtain the following materials: 1 fetal pig per group 1 large dissection pan 2 pieces of string - ~25-30 inches long 2 good scalpels Scissors 2
BLUNT
Probes Forceps (tweezers)
Initial Prep
• Open up the plastic bag – –
KEEP
the
outer bag
AND the
rubber band
!
– Cut open the inner bag over the sink. Empty pig and contents into sink. THROW AWAY inner bag into the
GREEN
trash • Rinse off pig well. Towel dry and place in a dissection tray
External Observations
• Look for the umbilical cord – Try to find the umbilical arteries/veins that connected its blood supply to mom via the placenta •
External nares
what those are?) (remember •
Tongue
• Boy or Girl?
– Look under the tail. • If all you see it an anus (hole) then a SMOOTH swollen sac of skin – you have found the
SCROTUM
and you have a boy!
• If you see an anus (hole) plus an obvious folded area of skin. You have found the
GENITAL PAPILLA
and you have girl!
Genital Papilla GIRL!!!
Scrotum BOY!
Anus Anus
Securing your Pig to the tray
• Use the 2 pieces of string to secure your pig to the dissecting tray
Incision Time
1. Take a scalpel cord – make a SUPERFICIAL midsaggital incision from chin to umbilical 2. Then use scissors to cut along either side of the umbilical cord to create a “flap” 3. & 4. Use scissors to make 2 lateral cuts. Avoid the staples on one side of the pig – Also be careful not to demolish the
DIAPHRAGM
at the base of the rib cage!
Incision Time
3. & 4. Use scissors to make 2 lateral cuts. Avoid the staples on one side of the pig – Also be careful not to demolish the
DIAPHRAGM
at the base of the rib cage!
Diaphragm
Separate Skin from rib cage
• Using your scapel – separate the skin of the chest area off of the rib cage.
– You will have to cut through muscle and connective tissue to do this – It’s like skinning raw chicken!
• Using scissors – cut of each half of the rib cage. You should end up with 2 large chunks of ribs in your tray
Diaphragm
• Use scissors to separate the diaphragm from the floor of the rib cage.
Diaphragm
• You should be left with one big “flap” of Diaphragm that hangs over the liver and stomach (see d
page 409 of lab book
)
Diaphragm
Exposing Larynx and Trachea – pg. 409
• Using scissors – cut off any remaining pericardial sac that might be covering the heart.
– Place any pieces in your tray for later disposal • Using a SHARP scalpel – remove all muscle and tissue overlying the larynx and trachea
Larynx Trachea
Neck Blood Vessels – pg. 418
• Using a
BLUNT PROBE
– carefully probe on either side of the trachea until you expose –
1 PINK common carotid artery
on each side –
2 BLUE jugular veins
on each side • These are the
internal and external jugular veins Common Carotid Artery Internal Jugular Vein External Jugular Vein
Heart
• Using a combination of scalpel and scissors: – Cut off the
pericardial sac
that surrounds the heart – Be careful not to cut off any important blood vessels!
Superior Vena Cava
• Also not the texture and position of the
RIGHT AND LEFT LUNGS.
Right Lung Left Lung
Cardiovascular System: Superior Vena Cava, Pulmonary Trunk & Aortic Arch
Superior Vena Cava Aortic Arch Pulmonary Trunk Right Lung Left Lung
What now? Locate all Respiratory & Digestive System organs listed on
Page 13 of your lab packet!
• Use
pg. 404 & pg. 409
in your lab books for help • Note: do NOT find the urinary organs yet – we will do this later.
• When your group thinks it has found everything – call me over so I can review it with you!
Ready to clean up?
• Cut off strings – throw away in
GREEN
trash.
• Throw any
PIG TISSUE WASTE
in the
RED
trash • Rinse pig under water and clean both external and internal areas.
• Spray pig with cavicide (in white spray bottle) inside and out!
• Put pig in your saved plastic bag and secure with rubber band. • Write the pig’s name (or group name) on the bag in permanent marker.
– Place bagged pig in the tray.
• Spray down your table with cavicide or simple green!
Respiratory System
• Make sure you can locate: – External nares – Larynx – Trachea – Diaphragm – lungs
Digestive System
• Be sure you can locate: – Tongue – Esophagus – Stomach – Liver – Gallbladder – Small intestine – Large Intestine – Pancreas – Mesentery – Rectum
Digestive System
Note: the following are not shown, but you need to know where they are:
Tongue, esophagus, stomach, pancreas Liver Gallbladder Small intestine Large intestine Mesentery Rectum
Fetal Pig Dissection – Day 2
Urinary System
• Do NOT cut out the intestines to dissect the urinary system!
• Push the intestines to one side and use a scalpel or blunt probe to remove the connective tissue around each
kidney
and
ureter
• Note the
ALLANTOIC BLADDER Right Kidney Right Ureter Rectum Left Kidney Left Ureter Allantoic Bladder
Reproductive System - MALE
Allantoic Bladder Spermatic Cord Penis testes Spermatic Cord Epididymis testes Epididymis
Reproductive System - FEMALE
Uterine Horn Uterine Horn Ovary Ovary Uterine Body Allantoic Bladder
Cardiovascular System – Heart & Spleen
heart Spleen
Cardiovascular System – page 418 – –
1 PINK common carotid artery
on each side
2 BLUE jugular veins
on each side • These are the
internal and external jugular veins Common Carotid Artery Internal Jugular Vein External Jugular Vein
Cardiovascular System: Superior Vena Cava, Pulmonary Trunk & Aortic Arch
Superior Vena Cava Aortic Arch Pulmonary Trunk Right Lung Left Lung
Cardiovascular System: Inferior Vena Cava & Abdominal Aorta
Inferior Vena Cava Mesentery Small Intestine Abdominal Aorta Rectum
Ready to clean up?
• Cut off strings – throw away in
GREEN
trash.
• Throw any
PIG TISSUE WASTE
in the
RED
trash • Rinse pig under water and clean both external and internal areas.
• Spray pig with cavicide (in white spray bottle) inside and out!
• Put pig in your saved plastic bag and secure with rubber band. • Write the pig’s name (or group name) on the bag in permanent marker.
– Place bagged pig in the tray.
• Spray down your table with cavicide or simple green!